-
Immunogen
A synthesized peptide derived from human NF-kappaB p105/p50
-
Antibody s target
Human NFKB1
-
Antibody s full description
Rabbit Anti-Human NFKB1 Polyclonal
-
Antibody s category
Polyclonal Antibodies
-
Antibody s other name
Embryonic stem cell specific homeobox protein (Nanog) Antibody, ENK Antibody, FLJ12581 Antibody, hNanog Antibody, Homeobox protein NANOG Antibody, Homeobox transcription factor Nanog Antibody, homeobox transcription factor Nanog-delta 48 Antibody, NANOG Antibody, Nanog homeobox Antibody, NANOG_HUMAN Antibody
-
Verified applications
WB, IHC, ICC/IF
-
Raised in
Rabbit
-
Antibody s reactivity
Human, Mouse
-
Antibody s recommended dilutions for use
WB (1:2000), IHC (1:200), ICC/IF (1:500); optimal dilutions for assays should be determined by the user.
-
Antibody s purified from
Peptide Affinity Purified
-
Recommended buffer for storage
PBS pH 7.4, 50% glycerol, 150mM NaCl, 0.02% sodium azide
-
Antibody s concentration
See included datasheet or contact our support service
-
Antibody s specificity
Detects ~50kDa, endogenous levels of total NF-kappaB p105/p50
-
Storage recommendations
-20°C
-
Shipping recommendations
Blue Ice or 4°C
-
Certificate of analysis
A 1:1000 dilution of SPC-1303 was sufficient for detection of NFKB1 in 10 µg of HeLa cell lysates by ECL immunoblot analysis using Goat Anti-Rabbit IgG:HRP as the secondary antibody.
-
Antibody in cell
Nucleus, Cytoplasm
-
Tissue specificity
See included datasheet or contact our support service.
-
Scientific context
Nuclear factor NF-kappa-B p105 subunit is a protein that in humans is encoded by the NFKB1 gene. Nuclear factor kappa beta (NFKB) is a transcription factor that is highly influenced by stress, free-radicals, UV light and hypoxia. Unsurprisingly, NFKB is essential for many human cancers, as it regulates the expression of many downstream proteins that regulate or modify cellular growth and development. NFKB effects on angiogenic pathways and reactive mechanisms to stress and damage are well established throughout literature. Atherosclerosis is partly attributed to the mis-regulation of NFKB in hypoxia. Arthritis is related to inflammation proteins regulated through NFKB. It also has been shown to be important in synapses in brain, leading to memory and cognitive ability or impairment. In all, NFKB is one of the fastest responding transcription factors in humans, and is likely to mediate many responsive events in diseases and injury recovery.
-
Bibliography
1. Meyer R. et al. (1991) Proc Natl Acad Sci U S A 88 (3): 966–70. 2. Gerdin AK et al. (2010) Acta Ophthalmologica 88: 925–7.
-
Released date
6/Sep/2016
-
NCBI number
NP_003989.2
-
Gene number
4790
-
Protein number
P19838
-
PubMed number
Refer to PubMed
-
Tested applications
To be tested
-
Tested reactivity
To be tested
-
Antibody s datasheet
Contact our support service to receive datasheet or other technical documentation.
-
-
Representative figure legend
Western blot analysis of Human HeLa cell lysates showing detection of ~105kDa NFKB1 protein using Rabbit Anti-NFKB1 Polyclonal Antibody (SPC-1303). Lane 1: TNF-a treated Human HeLa whole cell lysates treated with the immunizing peptide. Lane 2: TNF-a treated Human HeLa whole cell lysate. Primary Antibody: Rabbit Anti-NFKB1 Polyclonal Antibody (SPC-1303) at 1:1000. Predicted/Observed Size: ~105kDa. Rabbit Anti-NFKB1 Antibody used in Western blot (WB) on HeLa cell lysates (SPC-1303)
-
Warning information
Non-hazardous
-
Country of production
Canada
-
Total weight kg
1.4
-
Net weight g
0.1
-
Stock availabilit
In Stock
-
-
Description
This antibody needs to be stored at + 4°C in a fridge short term in a concentrated dilution. Freeze thaw will destroy a percentage in every cycle and should be avoided. Antibody for research use.
-
Test
StressMark antibodies supplies antibodies that are for research of human proteins.
-
Properties
Human proteins, cDNA and human recombinants are used in human reactive ELISA kits and to produce anti-human mono and polyclonal antibodies. Modern humans (Homo sapiens, primarily ssp. Homo sapiens sapiens). Depending on the epitopes used human ELISA kits can be cross reactive to many other species. Mainly analyzed are human serum, plasma, urine, saliva, human cell culture supernatants and biological samples.
-
Group
Polyclonals and antibodies
-
About
Polyclonals can be used for Western blot, immunohistochemistry on frozen slices or parrafin fixed tissues. The advantage is that there are more epitopes available in a polyclonal antiserum to detect the proteins than in monoclonal sera. Rabbits are used for polyclonal antibody production by StressMark antibodies. Rabbit antibodies are very stable and can be stored for several days at room temperature. StressMark antibodies adds sodium azide and glycerol to enhance the stability of the rabbit polyclonal antibodies. Anti-human, anti mouse antibodies to highly immunogenic selected peptide sequences are" monoclonal like" since the epitope to which they are directed is less than 35 amino acids long.
-
Latin name
Oryctolagus cuniculus
-
Gene target
-
Gene symbol
NFKB1
-
Short name
Rabbit Anti- NFKB1 Polyclonal: ATTO 633
-
Technique
anti-Human, Polyclonal, Rabbit, anti-, anti, antibody to, antibodies, antibodies against human proteins, Polyclonal antibodies (pAbs) are mostly rabbit or goat antibodies that are secreted by different B cells, whereas monoclonal antibodies come from a single N cell lineage. Pabs are a collection of immunoglobulin molecules that react against a specific antigen, each identifying a different epitope.
-
Host
Rabbit, Rabbits
-
Isotype
IgG
-
Label
ATTO 633
-
Species
Human, Humans
-
Alternative name
production species: rabbit Antibody toHuman nuclear factor on kappa light polypeptide gene enhancer in B-cells 1 polyclonal: ATTO 633
-
Alternative technique
polyclonals, rabbit-anti, antibodies
-
Alternative to gene target
nuclear factor of kappa light polypeptide gene enhancer in B-cells 1, EBP-1 and KBF1 and NF-kappa-B and NF-kappaB and NF-kB1 and NFkappaB and NFKB-p105 and NFKB-p50 and p105 and p50, NFKB1 and IDBG-31974 and ENSG00000109320 and 4790, transcription regulatory region DNA binding, nuclei, Nfkb1 and IDBG-195445 and ENSMUSG00000028163 and 18033, BT.60919 and IDBG-640840 and ENSBTAG00000020270 and 616115
-
