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Immunogen
Synthesized peptide derived from human MCL1.
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Antibody s target
Human MCL1
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Antibody s full description
Rabbit Anti-Human MCL1 Polyclonal
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Antibody s category
Polyclonal Antibodies
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Antibody s other name
Bcl 2 related protein EAT/mcl1 antibody, Bcl-2-like protein 3 antibody, Bcl-2-related protein EAT/mcl1 antibody, BCL2 related antibody, Bcl2-L-3 antibody, BCL2L3 antibody, EAT antibody, Induced myeloid leukemia cell differentiation protein Mcl 1 antibody, Induced myeloid leukemia cell differentiation protein Mcl-1 antibody, MCL 1 antibody, MCL1 antibody, MCL1-ES antibody, mcl1/EAT antibody, MCL1_HUMAN antibody, MCL1L antibody, MCL1S antibody, MGC104264 antibody, MGC1839 antibody, Myeloid Cell Leukemia 1 antibody, Myeloid cell leukemia ES antibody, Myeloid cell leukemia sequence 1 antibody, Myeloid cell leukemia sequence 1 BCL2 related antibody, Myeloid cell leukemia sequence 1 isoform 1 antibody, OTTHUMP00000032794 antibody, OTTHUMP00000032795 antibody, TM antibody
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Verified applications
WB, IHC, ELISA
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Raised in
Rabbit
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Antibody s reactivity
Human, Mouse, Rat
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Antibody s recommended dilutions for use
WB (1:1000), IHC (1:100), ELISA (1:40000); optimal dilutions for assays should be determined by the user.
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Antibody s purified from
Peptide Affinity Purified
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Recommended buffer for storage
PBS pH 7.4, 50% glycerol, 150mM NaCl, 0.02% sodium azide
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Antibody s concentration
See included datasheet or contact our support service
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Antibody s specificity
Detects endogenous levels of total MCL1 protein.
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Storage recommendations
-20°C
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Shipping recommendations
Blue Ice or 4°C
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Certificate of analysis
A 1:1000 dilution of SPC-1328 was sufficient for detection of MCL1 in 10 µg of HuvEc cell lysates by ECL immunoblot analysis using Goat Anti-Rabbit IgG:HRP as the secondary antibody.
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Antibody in cell
Membrane, Cytoplasm, Mitochondrion, Nucleus
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Tissue specificity
See included datasheet or contact our support service.
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Scientific context
Induced myeloid leukemia cell differentiation protein Mcl-1 is a protein that in humans is encoded by the MCL1 gene. It is involved in the regulation of apoptosis versus cell survival, and in the maintenance of viability but not of proliferation. Mediates its effects by interactions with a number of other regulators of apoptosis. Isoform 1 inhibits apoptosis. Isoform 2 promotes apoptosis.
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Bibliography
1. Kozopas KM. et al. (1993) Proc Natl Acad Sci U S A 90 (8): 3516–20. 2. Craig RW. et al. (1995) Genomics 23 (2): 457–63.
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Released date
6/Sep/2016
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NCBI number
NP_068779.1
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Gene number
4170
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Protein number
Q07820
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PubMed number
Refer to PubMed
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Tested applications
To be tested
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Tested reactivity
To be tested
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Antibody s datasheet
Contact our support service to receive datasheet or other technical documentation.
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Representative figure link
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Representative figure legend
Immunohistochemistry analysis using Rabbit Anti-MCL1 Polyclonal Antibody (SPC-1328). Tissue: Breast Carcinoma Tissue. Species: Human. Fixation: Formalin fixed paraffin-embedded. Primary Antibody: Rabbit Anti-MCL1 Polyclonal Antibody (SPC-1328) at 1:100. The image on the right is treated with the synthesized peptide. | Western blot analysis of Human HuvEc cell lysates showing detection of ~40kDa MCL1 protein using Rabbit Anti-MCL1 Polyclonal Antibody (SPC-1328). Lane 1: Human HuvEc cells. Lane 2: Human HuvEc cells treated with the immunizing peptide. Primary Antibody: Rabbit Anti-MCL1 Polyclonal Antibody (SPC-1328) at 1:1000. Predicted/Observed Size: ~40kDa. Rabbit Anti-MCL1 Antibody used in Immunohistochemistry (IHC) on Breast Carcinoma Tissue (SPC-1328) | Rabbit Anti-MCL1 Antibody used in Western blot (WB) on HuvEc cell lysates (SPC-1328)
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Warning information
Non-hazardous
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Country of production
Canada
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Total weight kg
1.4
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Net weight g
0.1
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Stock availabilit
In Stock
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Description
This antibody needs to be stored at + 4°C in a fridge short term in a concentrated dilution. Freeze thaw will destroy a percentage in every cycle and should be avoided. Antibody for research use.
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Test
StressMark antibodies supplies antibodies that are for research of human proteins.
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Properties
Human proteins, cDNA and human recombinants are used in human reactive ELISA kits and to produce anti-human mono and polyclonal antibodies. Modern humans (Homo sapiens, primarily ssp. Homo sapiens sapiens). Depending on the epitopes used human ELISA kits can be cross reactive to many other species. Mainly analyzed are human serum, plasma, urine, saliva, human cell culture supernatants and biological samples.
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Conjugate
Peridinin chlorophyll (PerCP)
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Group
Polyclonals and antibodies
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About
Polyclonals can be used for Western blot, immunohistochemistry on frozen slices or parrafin fixed tissues. The advantage is that there are more epitopes available in a polyclonal antiserum to detect the proteins than in monoclonal sera. Rabbits are used for polyclonal antibody production by StressMark antibodies. Rabbit antibodies are very stable and can be stored for several days at room temperature. StressMark antibodies adds sodium azide and glycerol to enhance the stability of the rabbit polyclonal antibodies. Anti-human, anti mouse antibodies to highly immunogenic selected peptide sequences are" monoclonal like" since the epitope to which they are directed is less than 35 amino acids long.
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Latin name
Oryctolagus cuniculus
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Gene target
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Gene symbol
MCL1
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Short name
Rabbit Anti- MCL1 Polyclonal: PerCP
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Technique
anti-Human, Polyclonal, Rabbit, anti-, Percp, anti, antibody to, antibodies, antibodies against human proteins, Peridinin chlorophyll protein (PerCP) has a 677 nanometer maximum emission, red, when excited at 488 nanometers and the Rabbit Anti- MCL1 Polyclonal: PerCP is detected on the FL3 detector of a FACSCalibur or FACScan. A PerCP dye is also available (PerCP-Cy5.5, also written Cy5.5-PerCP). PerCP is not suited for the high-powered lasing (>150mW) applications, such as on a MoFlo, due to it photobleaching (features/ qualities/ traits). PerCP conjugates can only be received/be gotten from the Becton Dickinson/Pharmingen group of companies or from StressMark antibodies. Polyclonal antibodies (pAbs) are mostly rabbit or goat antibodies that are secreted by different B cells, whereas monoclonal antibodies come from a single N cell lineage. Pabs are a collection of immunoglobulin molecules that react against a specific antigen, each identifying a different epitope.
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Host
Rabbit, Rabbits
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Isotype
IgG
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Label
PerCP
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Species
Human, Humans
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Alternative name
production species: rabbit Antibody toHuman myeloid cell leukemia sequence 1 (BCL2-related) polyclonal: PerCP
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Alternative technique
polyclonals, rabbit-anti, antibodies-percp, antibodies
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Alternative to gene target
myeloid cell leukemia sequence 1 (BCL2-related), bcl2-L-3 and BCL2L3 and EAT and Mcl-1 and MCL1-ES and mcl1/EAT and MCL1L and MCL1S and TM, MCL1 and IDBG-102145 and ENSG00000143384 and 4170, BH3 domain binding, nuclei, Mcl1 and IDBG-173221 and ENSMUSG00000038612 and 17210
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