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Immunogen
Peptide sequence around aa.291~295(G-G-R-D-S
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Antibody s target
Human MBP
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Antibody s full description
Rabbit Anti-Human MBP Polyclonal
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Antibody s category
Polyclonal Antibodies
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Antibody s other name
GDB Antibody, Golli MBP Antibody, Golli MBP; myelin basic protein Antibody, Hemopoietic MBP Antibody, HMBPR Antibody, HUGO Antibody, MBP Antibody, MBP_HUMAN Antibody, MGC99675 Antibody, MLD Antibody, Myelin A1 protein Antibody, Myelin basic protein Antibody, Myelin Deficient Antibody, Myelin membrane encephalitogenic protein Antibody, OTTHUMP00000163776 Antibody, OTTHUMP00000174387 Antibody, OTTHUMP00000174388 Antibody, SHI Antibody, Shiverer Antibody, SP Antibody
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Verified applications
WB
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Raised in
Rabbit
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Antibody s reactivity
Human, Mouse, Rat
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Antibody s recommended dilutions for use
WB (1:1000); optimal dilutions for assays should be determined by the user.
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Antibody s purified from
Peptide Affinity Purified
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Recommended buffer for storage
PBS pH 7.4, 50% glycerol, 150mM NaCl, 0.02% sodium azide
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Antibody s concentration
See included datasheet or contact our support service
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Antibody s specificity
Detects ~19kDa and ~26kDa, endogenous level of total MBP(myelin basic protein) protein.
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Storage recommendations
-20°C
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Shipping recommendations
Blue Ice or 4°C
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Certificate of analysis
A 1:1000 dilution of SPC-1301 was sufficient for detection of MBP in 10 µg of Brain cell lysates by ECL immunoblot analysis using Goat Anti-Rabbit IgG:HRP as the secondary antibody.
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Antibody in cell
Membrane
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Tissue specificity
MBP isoforms are found in both the central and the peripheral nervous system, whereas Golli-MBP isoforms are expressed in fetal thymus, spleen and spinal cord, as well as in cell lines derived from the immune system.
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Scientific context
Myelin basic protein (MBP) is a protein believed to be important in the process of myelination of nerves in the nervous system. The protein encoded by the classic MBP gene is a major constituent of the myelin sheath of oligodendrocytes and Schwann cells in the nervous system. However, MBP-related transcripts are also present in the bone marrow and the immune system. The classic group of MBP isoforms (isoform 4-isoform 14) are with PLP the most abundant protein components of the myelin membrane in the CNS. They have a role in both its formation and stabilization. The smaller isoforms might have an important role in remyelination of denuded axons in multiple sclerosis. The non-classic group of MBP isoforms (isoform 1-isoform 3/Golli-MBPs) may preferentially have a role in the early developing brain long before myelination, maybe as components of transcriptional complexes, and may also be involved in signaling pathways in T-cells and neural cells.
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Bibliography
1. Sakamoto Y. et al. (1987) J. Biol. Chem. 262 (9): 4208–14. 2. Deber CM and Reynolds SJ. (1991) Clin. Biochem. 24 (2): 113–34. 3. Inouye H and Kirschner DA. (1991) J. Neurosci. Res. 28 (1): 1–17. 4. Eylar EH et al. (1971) J. Biol. Chem. 246 (18): 5770–84. 5. Berger T et al. (2003) N. Engl. J. Med. 349 (2): 139–45.
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Released date
6/Sep/2016
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NCBI number
NP_002376.1
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Gene number
4155
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Protein number
P02686
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PubMed number
Refer to PubMed
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Tested applications
To be tested
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Tested reactivity
To be tested
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Antibody s datasheet
Contact our support service to receive datasheet or other technical documentation.
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Representative figure legend
Western blot analysis of Rat, Mouse Brain cell lysates showing detection of ~14-33kDa MBP protein using Rabbit Anti-MBP Polyclonal Antibody (SPC-1301). Lane 1: Rat Brain. Lane 2: Mouse Brain. Lane 3: Rat Hippocampus. Primary Antibody: Rabbit Anti-MBP Polyclonal Antibody (SPC-1301) at 1:1000. Predicted/Observed Size: ~14-33kDa. Rabbit Anti-MBP Antibody used in Western blot (WB) on Brain cell lysates (SPC-1301)
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Warning information
Non-hazardous
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Country of production
Canada
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Total weight kg
1.4
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Net weight g
0.1
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Stock availabilit
In Stock
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Description
This antibody needs to be stored at + 4°C in a fridge short term in a concentrated dilution. Freeze thaw will destroy a percentage in every cycle and should be avoided. Antibody for research use.
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Test
StressMark antibodies supplies antibodies that are for research of human proteins.
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Properties
Human proteins, cDNA and human recombinants are used in human reactive ELISA kits and to produce anti-human mono and polyclonal antibodies. Modern humans (Homo sapiens, primarily ssp. Homo sapiens sapiens). Depending on the epitopes used human ELISA kits can be cross reactive to many other species. Mainly analyzed are human serum, plasma, urine, saliva, human cell culture supernatants and biological samples.
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Group
Polyclonals and antibodies
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About
Polyclonals can be used for Western blot, immunohistochemistry on frozen slices or parrafin fixed tissues. The advantage is that there are more epitopes available in a polyclonal antiserum to detect the proteins than in monoclonal sera. Rabbits are used for polyclonal antibody production by StressMark antibodies. Rabbit antibodies are very stable and can be stored for several days at room temperature. StressMark antibodies adds sodium azide and glycerol to enhance the stability of the rabbit polyclonal antibodies. Anti-human, anti mouse antibodies to highly immunogenic selected peptide sequences are" monoclonal like" since the epitope to which they are directed is less than 35 amino acids long.
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Latin name
Oryctolagus cuniculus
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Gene target
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Gene symbol
MBP, PRG2, ENO1, HIVEP2, HIVEP1
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Short name
Rabbit Anti- MBP Polyclonal: ATTO 565
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Technique
anti-Human, Polyclonal, Rabbit, anti-, anti, antibody to, antibodies, antibodies against human proteins, Polyclonal antibodies (pAbs) are mostly rabbit or goat antibodies that are secreted by different B cells, whereas monoclonal antibodies come from a single N cell lineage. Pabs are a collection of immunoglobulin molecules that react against a specific antigen, each identifying a different epitope.
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Host
Rabbit, Rabbits
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Isotype
IgG
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Label
ATTO 565
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Species
Human, Humans
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Alternative name
production species: rabbit Antibody toHuman MBP polyclonal: ATTO 565
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Alternative technique
polyclonals, rabbit-anti, antibodies
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