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Immunogen
A phospho-specific peptide corresponding to residues surrounding Tyr156 and Thr157 of human ICK (AA152-158)
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Antibody s target
Human ICK (pTyr156 + pThr157)
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Antibody s full description
Rabbit Anti-Human ICK (pTyr156 + pThr157) Polyclonal
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Antibody s category
Polyclonal Antibodies
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Antibody s other name
ECO Antibody, Heart serine threonine protein kinase Antibody, hICK Antibody, Intestinal cell (MAK like) kinase Antibody, Intestinal cell kinase Antibody, Laryngeal cancer kinase 2 Antibody, LCK2 Antibody, MAK related kinase Antibody, MGC46090 Antibody, mICK Antibody, MRK Antibody, Serine/threonine protein kinase ICK Antibody
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Verified applications
WB, AM
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Raised in
Rabbit
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Antibody s reactivity
Human
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Antibody s recommended dilutions for use
WB (1:250); optimal dilutions for assays should be determined by the user.
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Antibody s purified from
Peptide Affinity Purified
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Recommended buffer for storage
PBS pH7.4, 50% glycerol, 0.025% Thimerosal
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Antibody s concentration
See included datasheet or contact our support service
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Antibody s specificity
Detects 71.427 kDa.
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Storage recommendations
-20°C
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Shipping recommendations
Blue Ice or 4°C
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Certificate of analysis
A 1:250 dilution of SPC-996 was sufficient for detection of ICK (pTyr156 + pThr157) in 10 µg of HeLa cell lysate by ECL immunoblot analysis using goat anti-rabbit IgG:HRP as the secondary antibody.
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Antibody in cell
Nucleus, Cytoplasm, Cytosol, Cytoskeleton
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Tissue specificity
Expressed in heart, brain, placenta, pancreas, thymus, prostate, testis, ovary, small intestine and colon, with highest levels in placenta and testis. Not detected in spleen. Also expressed in many cancer cell lines
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Scientific context
ICK1 encodes an intestinal serine/threonine kinase harboring a dual phosphorylation site found in mitogen-activating protein (MAP) kinases. The protein localizes to the intestinal crypt region and is thought to be important in intestinal epithelial cell proliferation and differentiation.
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Bibliography
Refer to PubMed
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Released date
29/Aug/2016
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NCBI number
NP_055735
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Gene number
22858
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Protein number
Q9UPZ9
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PubMed number
Refer to PubMed
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Tested applications
To be tested
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Tested reactivity
To be tested
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Antibody s datasheet
Contact our support service to receive datasheet or other technical documentation.
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Representative figure link
No Data Available
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Warning information
Non-hazardous
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Country of production
Canada
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Total weight kg
1.4
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Net weight g
0.1
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Stock availabilit
In Stock
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Description
This antibody needs to be stored at + 4°C in a fridge short term in a concentrated dilution. Freeze thaw will destroy a percentage in every cycle and should be avoided. Antibody for research use.
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Test
StressMark antibodies supplies antibodies that are for research of human proteins.
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Properties
Human proteins, cDNA and human recombinants are used in human reactive ELISA kits and to produce anti-human mono and polyclonal antibodies. Modern humans (Homo sapiens, primarily ssp. Homo sapiens sapiens). Depending on the epitopes used human ELISA kits can be cross reactive to many other species. Mainly analyzed are human serum, plasma, urine, saliva, human cell culture supernatants and biological samples.
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Group
Polyclonals and antibodies
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About
Polyclonals can be used for Western blot, immunohistochemistry on frozen slices or parrafin fixed tissues. The advantage is that there are more epitopes available in a polyclonal antiserum to detect the proteins than in monoclonal sera. Rabbits are used for polyclonal antibody production by StressMark antibodies. Rabbit antibodies are very stable and can be stored for several days at room temperature. StressMark antibodies adds sodium azide and glycerol to enhance the stability of the rabbit polyclonal antibodies. Anti-human, anti mouse antibodies to highly immunogenic selected peptide sequences are" monoclonal like" since the epitope to which they are directed is less than 35 amino acids long.
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Latin name
Oryctolagus cuniculus
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Gene target
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Gene symbol
CILK1
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Short name
Rabbit Anti- ICK (pTyr156 + pThr157) Polyclonal: PE/ATTO 594
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Technique
anti-Human, Polyclonal, Rabbit, anti-, anti, antibody to, antibodies, antibodies against human proteins, Polyclonal antibodies (pAbs) are mostly rabbit or goat antibodies that are secreted by different B cells, whereas monoclonal antibodies come from a single N cell lineage. Pabs are a collection of immunoglobulin molecules that react against a specific antigen, each identifying a different epitope.
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Host
Rabbit, Rabbits
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Isotype
Immunoglobulin
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Label
PE/ATTO 594
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Species
Human, Humans
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Alternative name
production species: rabbit Antibody toHuman intestinal cell (MAK-like) kinase (pTyr156 + pThr157) polyclonal: peroxidase/ATTO 594
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Alternative technique
polyclonals, rabbit-anti, antibodies
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Alternative to gene target
intestinal cell (MAK-like) kinase, ECO and LCK2 and MRK, ICK and IDBG-90915 and ENSG00000112144 and 22858, transferase activity, nuclei, Ick and IDBG-186294 and ENSMUSG00000009828 and 56542, ICK and IDBG-632721 and ENSBTAG00000015590 and 506286
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