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Immunogen
A phospho-specific peptide corresponding to residues surrounding Tyr263 and Tyr264 of human Lck (AA257-268)
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Antibody s target
Human Lck (pTyr263+pTyr264)
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Antibody s full description
Rabbit Anti-Human Lck (pTyr263+pTyr264) Polyclonal
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Antibody s category
Polyclonal Antibodies
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Antibody s other name
IMD22 Antibody, LCK Antibody, Lck p56 Antibody, LCK proto-oncogene, Src family tyrosine kinase Antibody, Leukocyte C-terminal Src kinase Antibody, LSK Antibody, Lymphocyte cell specific protein tyrosine kinase Antibody, Membrane associated protein tyrosine kinase Antibody, Oncogene lck Antibody, P56 LCK Antibody, p56lck Antibody, pp58 lck Antibody, Protein YT16 Antibody, Proto oncogene tyrosine protein kinase LCK Antibody, Proto-oncogene Lck Antibody, T cell specific protein tyrosine kinase Antibody, T lymphocyte specific protein tyrosine kinase p56lck Antibody, Tyrosine-protein kinase Lck Antibody, YT 16 Antibody
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Verified applications
WB, AM
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Raised in
Rabbit
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Antibody s reactivity
Human
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Antibody s recommended dilutions for use
WB (1:250); optimal dilutions for assays should be determined by the user.
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Antibody s purified from
Peptide Affinity Purified
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Recommended buffer for storage
PBS pH7.4, 50% glycerol, 0.025% Thimerosal
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Antibody s concentration
See included datasheet or contact our support service
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Antibody s specificity
Detects 57.869 kDa.
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Storage recommendations
-20°C
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Shipping recommendations
Blue Ice or 4°C
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Certificate of analysis
A 1:250 dilution of SPC-1006 was sufficient for detection of Lck (pTyr263+pTyr264) in 10 µg of Jurkat cell lysate by ECL immunoblot analysis using goat anti-rabbit IgG:HRP as the secondary antibody.
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Antibody in cell
Cytoplasm, Cell Membrane
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Tissue specificity
Expressed specifically in lymphoid cells.
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Scientific context
LCK is a protein-tyrosine kinase involved in T-cell development and T-cell activation.
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Bibliography
Refer to PubMed
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Released date
29/Aug/2016
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NCBI number
NP_005347
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Gene number
3932
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Protein number
P06239
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PubMed number
Refer to PubMed
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Tested applications
To be tested
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Tested reactivity
To be tested
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Antibody s datasheet
Contact our support service to receive datasheet or other technical documentation.
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Representative figure link
No Data Available
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Warning information
Non-hazardous
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Country of production
Canada
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Total weight kg
1.4
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Net weight g
0.1
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Stock availabilit
In Stock
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Description
This antibody needs to be stored at + 4°C in a fridge short term in a concentrated dilution. Freeze thaw will destroy a percentage in every cycle and should be avoided. Antibody for research use.
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Test
StressMark antibodies supplies antibodies that are for research of human proteins.
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Properties
Human proteins, cDNA and human recombinants are used in human reactive ELISA kits and to produce anti-human mono and polyclonal antibodies. Modern humans (Homo sapiens, primarily ssp. Homo sapiens sapiens). Depending on the epitopes used human ELISA kits can be cross reactive to many other species. Mainly analyzed are human serum, plasma, urine, saliva, human cell culture supernatants and biological samples.
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Group
Polyclonals and antibodies
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About
Polyclonals can be used for Western blot, immunohistochemistry on frozen slices or parrafin fixed tissues. The advantage is that there are more epitopes available in a polyclonal antiserum to detect the proteins than in monoclonal sera. Rabbits are used for polyclonal antibody production by StressMark antibodies. Rabbit antibodies are very stable and can be stored for several days at room temperature. StressMark antibodies adds sodium azide and glycerol to enhance the stability of the rabbit polyclonal antibodies. Anti-human, anti mouse antibodies to highly immunogenic selected peptide sequences are" monoclonal like" since the epitope to which they are directed is less than 35 amino acids long.
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Latin name
Oryctolagus cuniculus
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Conjugation
not conjugated
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Gene target
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Short name
Rabbit Anti- Lck (pTyr263+pTyr264) Polyclonal: Unconjugated
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Technique
anti-Human, Polyclonal, Rabbit, anti-, anti, antibody to, antibodies, antibodies against human proteins, Polyclonal antibodies (pAbs) are mostly rabbit or goat antibodies that are secreted by different B cells, whereas monoclonal antibodies come from a single N cell lineage. Pabs are a collection of immunoglobulin molecules that react against a specific antigen, each identifying a different epitope.
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Host
Rabbit, Rabbits
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Isotype
Immunoglobulin
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Label
Unconjugated
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Species
Human, Humans
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Alternative name
production species: rabbit Antibody toHuman lymphocyte-specific protein tyrosine kinase (pTyr263andpTyr264) polyclonal: nonconjugated
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Alternative technique
polyclonals, rabbit-anti, antibodies
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Alternative to gene target
lymphocyte-specific protein tyrosine kinase, IMD22 and LSK and p56lck and pp58lck and YT16, LCK and IDBG-95667 and ENSG00000182866 and 3932, transferase activity, Cell surfaces, Lck and IDBG-191117 and ENSMUSG00000000409 and 16818, LCK and IDBG-644592 and ENSBTAG00000012695 and 508890
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