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Immunogen
Synthesized peptide derived from human iNOS.
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Antibody s target
Human iNOS
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Antibody s full description
Rabbit Anti-Human iNOS Polyclonal
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Antibody s category
Polyclonal Antibodies
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Antibody s other name
HEP-NOS antibody, Hepatocyte NOS antibody, HEPNOS antibody, inducible antibody, Inducible nitric oxide synthase antibody, Inducible NO synthase antibody, Inducible NOS antibody, iNOS antibody, MAC NOS antibody, Macrophage NOS antibody, Nitric oxide synthase 2 inducible antibody, Nitric oxide synthase 2 inducible macrophage antibody, nitric oxide synthase 2A (inducible, hepatocytes) antibody, Nitric oxide synthase antibody, Nitric oxide synthase inducible antibody, nitric oxide synthase, macrophage antibody, NOS 2 antibody, NOS antibody, Nos II antibody, NOS type II antibody, nos2 antibody, NOS2_HUMAN antibody, NOS2A antibody, NOS2A, Inducible, Hepatocyte antibody, Peptidyl-cysteine S-nitrosylase NOS2 antibody
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Verified applications
WB, IHC, ICC/IF, ELISA
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Raised in
Rabbit
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Antibody s reactivity
Human
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Antibody s recommended dilutions for use
WB (1:1000), IHC (1:100), ICC/IF (1:500), ELISA (1:20000); optimal dilutions for assays should be determined by the user.
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Antibody s purified from
Peptide Affinity Purified
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Recommended buffer for storage
PBS pH 7.4, 50% glycerol, 150mM NaCl, 0.02% sodium azide
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Antibody s concentration
See included datasheet or contact our support service
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Antibody s specificity
Detects endogenous levels of total iNOS protein.
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Storage recommendations
-20°C
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Shipping recommendations
Blue Ice or 4°C
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Certificate of analysis
A 1:1000 dilution of SPC-1325 was sufficient for detection of iNOS in 10 µg of NIH/3T3 cell lysates by ECL immunoblot analysis using Goat Anti-Rabbit IgG:HRP as the secondary antibody.
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Antibody in cell
Cytoplasm, Nucleus, Peroxisome
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Tissue specificity
See included datasheet or contact our support service.
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Scientific context
Nitric oxide (NO) is an inorganic, gaseous free radical that carries a variety of messages between cells. Vasorelaxation, neurotransmission and cytotoxicity can all be potentiated through cellular response to NO. NO production is mediated by members of the nitric oxide synthase (NOS) family. NOS catalyzes the oxidization of L-arginine to produce L-citrulline and NO. Two constitutive isoforms, brain or neuronal NOS (b or nNOS, type I) & endothelial cell NOS (eNOS, type III), and one inducible isoform (iNOS, type II), have been cloned.
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Bibliography
1. Knowles RG and Moncada S. (1994) Biochem. J. 298 (2): 249–58. 2. Burger DE. et al. (2009) Circulation 120 (14): 1345–54. 3. Taylor BS. (1997) Arch Surg 132 (11): 1177–83.
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Released date
6/Sep/2016
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NCBI number
NP_000616.3
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Gene number
4843
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Protein number
P35228
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PubMed number
Refer to PubMed
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Tested applications
To be tested
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Tested reactivity
To be tested
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Antibody s datasheet
Contact our support service to receive datasheet or other technical documentation.
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Representative figure link
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Representative figure legend
Immunohistochemistry analysis using Rabbit Anti-iNOS Polyclonal Antibody (SPC-1325). Tissue: Lung Carcinoma Tissue. Species: Human. Fixation: Formalin fixed paraffin-embedded. Primary Antibody: Rabbit Anti-iNOS Polyclonal Antibody (SPC-1325) at 1:100. The image on the right is treated with the synthesized peptide. | Immunocytochemistry/Immunofluorescence analysis using Rabbit Anti-iNOS Polyclonal Antibody (SPC-1325). Tissue: HepG2 cells. Species: Human. Primary Antibody: Rabbit Anti-iNOS Polyclonal Antibody (SPC-1325) at 1:100. The image on the right is treated with the synthesized peptide. | Western blot analysis of Mouse NIH/3T3 cell lysates showing detection of ~131kDa iNOS protein using Rabbit Anti-iNOS Polyclonal Antibody (SPC-1325). Lane 1: Mouse NIH/3T3. Lane 2: Mouse NIH/3T3 treated with the immunizing peptide. Primary Antibody: Rabbit Anti-iNOS Polyclonal Antibody (SPC-1325) at 1:1000. Predicted/Observed Size: ~131kDa. Rabbit Anti-iNOS Antibody used in Immunohistochemistry (IHC) on Lung Carcinoma Tissue (SPC-1325) | Rabbit Anti-iNOS Antibody used in Immunocytochemistry/Immunofluorescence (ICC/IF) on HepG2 cells (SPC-1325) | Rabbit Anti-iNOS Antibody used in Western blot (WB) on NIH/3T3 cell lysates (SPC-1325)
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Warning information
Non-hazardous
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Country of production
Canada
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Total weight kg
1.4
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Net weight g
0.1
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Stock availabilit
In Stock
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Description
This antibody needs to be stored at + 4°C in a fridge short term in a concentrated dilution. Freeze thaw will destroy a percentage in every cycle and should be avoided. Antibody for research use.
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Test
StressMark antibodies supplies antibodies that are for research of human proteins.
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Properties
Human proteins, cDNA and human recombinants are used in human reactive ELISA kits and to produce anti-human mono and polyclonal antibodies. Modern humans (Homo sapiens, primarily ssp. Homo sapiens sapiens). Depending on the epitopes used human ELISA kits can be cross reactive to many other species. Mainly analyzed are human serum, plasma, urine, saliva, human cell culture supernatants and biological samples.
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Group
Polyclonals and antibodies
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About
Polyclonals can be used for Western blot, immunohistochemistry on frozen slices or parrafin fixed tissues. The advantage is that there are more epitopes available in a polyclonal antiserum to detect the proteins than in monoclonal sera. Rabbits are used for polyclonal antibody production by StressMark antibodies. Rabbit antibodies are very stable and can be stored for several days at room temperature. StressMark antibodies adds sodium azide and glycerol to enhance the stability of the rabbit polyclonal antibodies. Anti-human, anti mouse antibodies to highly immunogenic selected peptide sequences are" monoclonal like" since the epitope to which they are directed is less than 35 amino acids long.
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Latin name
Oryctolagus cuniculus
