Rabbit anti-human IgG Fab fragment polyclonal Antibody;FITC conjugated

  • Catalog number
    OACA00292
  • Price
    Please ask
  • Size
    one vial
  • Recognized antigen
    Rabbit human IgG Fab fragment polyclonal;FITC conjugated
  • Product type
    Antibody
  • Gene name
    Rabbit human IgG Fab fragment polyclonal;FITC conjugated
  • Long name
    Antibody against Rabbit human IgG Fab fragment polyclonal;FITC conjugated
  • Storage
    For shorter time periods the Rabbit human IgG Fab fragment polyclonal;FITC conjugated Antibodies may be kept refrigerated at +4 degrees Celsius. For extended periods of time keep frozen at -20 °C. Avoid freezing and thawing the lysate repeatedly as these cycles will cause denaturation and/or disruption of the protein chains and peptide bonds of the Rabbit human IgG Fab fragment polyclonal;FITC conjugated Antibodies which might cause change or loss of the desired properties.
  • Tips
    Small amounts of the Rabbit human IgG Fab fragment polyclonal;FITC conjugated antibody may get entrapped on the walls or lid of the vial as a result of shaking during transportation or handling. Prior to use, cetrifuge the vial to ensure that all of its content is collected on the bottom. In order to avoid cycles of freezing and thawing we recommend to plan and prepare aliquots according to the experiment demand and defrost only the amount which wil be needed for immediate use.
  • Description
    This antibody needs to be stored at + 4°C in a fridge short term in a concentrated dilution. Freeze thaw will destroy a percentage in every cycle and should be avoided. Antibody for research use.
  • Properties
    If you buy Antibodies supplied by aviva they should be stored frozen at - 24°C for long term storage and for short term at + 5°C. Human Fab Of Normal IgG Chromatographically purified Fab fragment of human IgG. Purified human Fab may be used as a reference antigen, calibrator, coating protein and blocking agent, This aviva Fluorescein isothiocyanate (FITC) antibody is currently after some BD antibodies the most commonly used fluorescent dye for FACS. When excited at 488 nanometers, FITC has a green emission that's usually collected at 530 nanometers, the FL1 detector of a FACSCalibur or FACScan. FITC has a high quantum yield (efficiency of energy transfer from absorption to emission fluorescence) and approximately half of the absorbed photons are emitted as fluorescent light. For fluorescent microscopy applications, the 1 FITC is seldom used as it photo bleaches rather quickly though in flow cytometry applications, its photo bleaching effects are not observed due to a very brief interaction at the laser intercept. aviva FITC is highly sensitive to pH extremes. Human proteins, cDNA and human recombinants are used in human reactive ELISA kits and to produce anti-human mono and polyclonal antibodies. Modern humans (Homo sapiens, primarily ssp. Homo sapiens sapiens). Depending on the epitopes used human ELISA kits can be cross reactive to many other species. Mainly analyzed are human serum, plasma, urine, saliva, human cell culture supernatants and biological samples.
  • Test
    aviva supplies antibodies that are for research of human proteins.
  • Conjugation
    Anti-FITC Antibody
  • About
    Immunoglobulin gamma, IgG, mouse monoclonal H&L chain clones or rabbit, goat polyclonal antibodies have 4 parts. There are 2 heavy chains, 2 light chains. The IgG antibody has 2 antigen binding sites. They represent 70% or more of serum antibodies. This antibody can be antigen purified or protein A or G purified. For storage sodium azide is added or you can call us to request azide free antibody preparations. These will need colder storage temperatures. Polyclonals can be used for Western blot, immunohistochemistry on frozen slices or parrafin fixed tissues. The advantage is that there are more epitopes available in a polyclonal antiserum to detect the proteins than in monoclonal sera. Rabbits are used for polyclonal antibody production by aviva. Rabbit antibodies are very stable and can be stored for several days at room temperature. aviva adds sodium azide and glycerol to enhance the stability of the rabbit polyclonal antibodies. Anti-human, anti mouse antibodies to highly immunogenic selected peptide sequences are" monoclonal like" since the epitope to which they are directed is less than 35 amino acids long.
  • Group
    Polyclonals and antibodies
  • Latin name
    Oryctolagus cuniculus
  • French translation
    anticorps
  • Gene target
  • Short name
    Anti-Rabbit human IgG Fab fragment polyclonal;FITC conjugated
  • Technique
    anti-Human, Polyclonal, Antibody, Rabbit, anti-, anti, FITC, Fab, IgG, antibody to, antibodies, antibodies against human proteins, antibodies for, antibodies against human proteins, antibody Conjugates, fabs, Fluorescein, IgGs, Polyclonal antibodies (pAbs) are mostly rabbit or goat antibodies that are secreted by different B cells, whereas monoclonal antibodies come from a single N cell lineage. Pabs are a collection of immunoglobulin molecules that react against a specific antigen, each identifying a different epitope.
  • Host
    Rabbit, Rabbits
  • Isotype
    IgG,  Fab fragment, Antibody fragments and scFv fragments, single chain variable fragments can be E. coli expressed. Fragment peptides for polyclonals give monoclonal like antibodies as the epitope is very small. IgG Fc and(ab)2 fragments are small and stable subunits of the antibody obtained by enzymatic digestion. Complement fragments, bp DNA fragments are common. IgG
  • Label
    FITC
  • Species
    Human, Humans
  • Alternative name
    Rabbit human IgG Fab fragment polyclonal;FITC conjugated Antibodies
  • Alternative technique
    polyclonals, antibodies, rabbit-anti, fluorescine, seconairy, immunoglobulins
MeSH Data
  • Name
  • Concept
    Scope note: Identification of proteins or peptides that have been electrophoretically separated by blot transferring from the electrophoresis gel to strips of nitrocellulose paper, followed by labeling with antibody probes.
  • Tree numbers
    • E05.196.401.143
    • E05.301.300.096
    • E05.478.566.320.200
    • E05.601.262
    • E05.601.470.320.200
  • Qualifiers
    ethics, trends, veterinary, history, classification, economics, instrumentation, methods, standards, statistics & numerical data
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