Rabbit Anti-Human GABARAPL1 Polyclonal: HRP

  • Catalog number
    SPC-622D-HRP
  • Price
    Please ask
  • Size
    100 µg
  • Immunogen
    Synthetic peptide from the N-terminal of Human GABARAPL1 (aa. 5-16)
  • Antibody s target
    Human GABARAPL1
  • Antibody s full description
    Rabbit Anti-Human GABARAPL1 Polyclonal
  • Primary research fields
    Neuroscience, Neurotransmitter Receptors, GABA Receptors
  • Antibody s category
    Polyclonal Antibodies
  • Antibody s other name
    APG8 like Antibody, GEC-1 Antibody, ATG8 Antibody, Glandular epithelial cell protein 1 Antibody, ATG8B Antibody, GEC1 Antibody, GBRL1_HUMAN Antibody, Gamma-aminobutyric acid receptor-associated protein-like 1 Antibody, GABARAPL1 a Antibody, ATG8L Antibody, APG8L Antibody, GABA(A) receptor-associated protein-like 1 Antibody, Early estrogen regulated protein Antibody, Early estrogen-regulated protein Antibody, GABA(A) receptor associated protein like 1 Antibody, Gamma aminobutyric acid receptor associated protein like 1 Antibody, GEC 1 Antibody, GABARAPL1 Antibody, GABA Antibody,
  • Verified applications
    WB, ICC/IF
  • Raised in
    Rabbit
  • Antibody s reactivity
    Human, Mouse
  • Antibody s recommended dilutions for use
    WB (1:1000); ICC/IF (1:100); optimal dilutions for assays should be determined by the user.
  • Antibody s purified from
    Peptide Affinity Purified
  • Recommended buffer for storage
    PBS, 50% glycerol, 0.09% sodium azide
  • Antibody s concentration
    1 mg/ml
  • Antibody s specificity
    Detects ~18 kDa.
  • Storage recommendations
    -20°C
  • Shipping recommendations
    Blue Ice or 4°C
  • Certificate of analysis
    A 1:1000 dilution of SPC-622 was sufficient for detection of GABARAPL1 in 15 µg of Human HeLa Cell Lysates by ECL immunoblot analysis using goat anti-rabbit IgG:HRP as the secondary antibody.
  • Antibody in cell
    Cytoplasm, Cytoskeleton, Cytoplasmic Vesicle Membrane, Lipid-Anchor, Endoplasmic Reticulum, Golgi Apparatus, Cytoplasmic Vesicle, Autophagosome
  • Tissue specificity
    Ubiquitous. Expressed at very high levels in the brain, heart, peripheral blood leukocytes, liver, kidney, placenta and skeletal muscle. Expressed at very low levels in thymus and small intestine. In the brain, expression is particularly intense in motoneurons in the embryo and in neurons involved in somatomotor and neuroendocrine functions in the adult, particularly in the substantia nigra pars compacta.
  • Scientific context
    See included datasheet or contact our support service.
  • Bibliography
    Refer to PubMed
  • Released date
    29/Aug/2016
  • NCBI number
    NP_113600.1
  • Gene number
    23710
  • Protein number
    Q9H0R8
  • PubMed number
    Refer to PubMed
  • Tested applications
    To be tested
  • Tested reactivity
    To be tested
  • Antibody s datasheet
    Contact our support service to receive datasheet or other technical documentation.
  • Representative figure link
  • Representative figure legend
    Immunocytochemistry/Immunofluorescence analysis using Rabbit Anti-GABARAPL1 Polyclonal Antibody (SPC-622). Tissue: SK-N-BE Cells (Human Neuroblastoma cells). Species: Human. Fixation: 4% Formaldehyde for 15 min at RT. Primary Antibody: Rabbit Anti-GABARAPL1 Polyclonal Antibody (SPC-622) at 1:100 for 60 min at RT. Secondary Antibody: Goat Anti-Mouse ATTO 488 at 1:200 for 60 min at RT. Counterstain: Phalloidin Texas Red F-Actin stain; DAPI (blue) nuclear stain at 1:1000, 1:5000 for 60 min at RT, 5 min at RT. Localization: Cytoplasmic Vesicle, Autophagosome, Cytoplasm, Nucleus. Magnification: 60X. (A) DAPI (blue) nuclear stain (B) Phalloidin Texas Red F-Actin stain (C) GABARAPL1 Antibody (D) Composite. | Western blot analysis of Human HeLa and 293Trap cell lysates showing detection of 14 kDa GABARAPL1 protein using Rabbit Anti-GABARAPL1 Polyclonal Antibody (SPC-622). Lane 1: Molecular Weight Ladder (MW). Lane 2: Human HeLa and 293Trap cell lysates. Load: 15 µg . Block: 5% Skim Milk in 1X TBST. Primary Antibody: Rabbit Anti-GABARAPL1 Polyclonal Antibody (SPC-622) at 1:1000 for 1 hour at RT. Secondary Antibody: Goat Anti-Rabbit HRP at 1:2000 for 60 min at RT. Color Development: ECL solution for 6 min in RT. Predicted/Observed Size: 14 kDa. Rabbit Anti-GABARAPL1 Antibody used in Immunocytochemistry/Immunofluorescence (ICC/IF) on Human SK-N-BE Cells (Human Neuroblastoma cells) (SPC-622) | Rabbit Anti-GABARAPL1 Antibody used in Western blot (WB) on HeLa and 293Trap cell lysates (SPC-622)
  • Warning information
    Non-hazardous
  • Country of production
    Canada
  • Total weight kg
    1.4
  • Net weight g
    0.1
  • Stock availabilit
    In Stock
  • Description
    This antibody needs to be stored at + 4°C in a fridge short term in a concentrated dilution. Freeze thaw will destroy a percentage in every cycle and should be avoided. Antibody for research use.
  • Test
    StressMark antibodies supplies antibodies that are for research of human proteins.
  • Properties
    HRP conjugates are often primary rabbit polyclonal antibodies couples to the enzyme horseradish peroxidase (HRP), found in the roots of horseradish. Also monoclonal HRP conjugates are often used for Western Blot. Human proteins, cDNA and human recombinants are used in human reactive ELISA kits and to produce anti-human mono and polyclonal antibodies. Modern humans (Homo sapiens, primarily ssp. Homo sapiens sapiens). Depending on the epitopes used human ELISA kits can be cross reactive to many other species. Mainly analyzed are human serum, plasma, urine, saliva, human cell culture supernatants and biological samples.
  • Conjugation
    HRP Conjugated
  • Group
    Polyclonals and antibodies
  • About
    Polyclonals can be used for Western blot, immunohistochemistry on frozen slices or parrafin fixed tissues. The advantage is that there are more epitopes available in a polyclonal antiserum to detect the proteins than in monoclonal sera. Rabbits are used for polyclonal antibody production by StressMark antibodies. Rabbit antibodies are very stable and can be stored for several days at room temperature. StressMark antibodies adds sodium azide and glycerol to enhance the stability of the rabbit polyclonal antibodies. Anti-human, anti mouse antibodies to highly immunogenic selected peptide sequences are" monoclonal like" since the epitope to which they are directed is less than 35 amino acids long.
  • Latin name
    Oryctolagus cuniculus
  • Gene target
  • Gene symbol
    GABARAPL1-AS1, GABARAPL1
  • Short name
    Rabbit Anti- GABARAPL1 Polyclonal:
  • Technique
    anti-Human, Polyclonal, Rabbit, anti-, anti, antibody to, antibodies, antibodies against human proteins, Polyclonal antibodies (pAbs) are mostly rabbit or goat antibodies that are secreted by different B cells, whereas monoclonal antibodies come from a single N cell lineage. Pabs are a collection of immunoglobulin molecules that react against a specific antigen, each identifying a different epitope.
  • Host
    Rabbit, Rabbits
  • Isotype
    Immunoglobulin
  • Label
    HRP
  • Species
    Human, Humans
  • Alternative name
    production species: rabbit Antibody toHuman GABARAPL1 polyclonal: horseradish peroxidase
  • Alternative technique
    polyclonals, rabbit-anti, antibodies
Gene info
Gene info
MeSH Data
  • Name
  • Concept
    Scope note: Identification of proteins or peptides that have been electrophoretically separated by blot transferring from the electrophoresis gel to strips of nitrocellulose paper, followed by labeling with antibody probes.
  • Tree numbers
    • E05.196.401.143
    • E05.301.300.096
    • E05.478.566.320.200
    • E05.601.262
    • E05.601.470.320.200
  • Qualifiers
    ethics, trends, veterinary, history, classification, economics, instrumentation, methods, standards, statistics & numerical data
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