Rabbit Anti-Human GABARAP Polyclonal: Unconjugated

  • Catalog number
    SPC-620S
  • Price
    Please ask
  • Size
    12 µg
  • Immunogen
    Synthetic peptide from the N-terminal of human GABARAP
  • Antibody s target
    Human GABARAP
  • Antibody s full description
    Rabbit Anti-Human GABARAP Polyclonal
  • Primary research fields
    Neuroscience, Neurotransmitter Receptors, GABA Receptors
  • Antibody s category
    Polyclonal Antibodies
  • Antibody s other name
    ATG8A Antibody, GABARAP A Antibody, GABA(A) Receptor Associated Protein Antibody, MM46 Antibody
  • Verified applications
    WB, ICC/IF
  • Raised in
    Rabbit
  • Antibody s reactivity
    Human, Mouse
  • Antibody s recommended dilutions for use
    WB (1:1000); ICC/IF (1:100); optimal dilutions for assays should be determined by the user.
  • Antibody s purified from
    Peptide Affinity Purified
  • Recommended buffer for storage
    PBS, 50% glycerol, 0.09% sodium azide
  • Antibody s concentration
    1 mg/ml
  • Antibody s specificity
    Detects ~14-16kDa.
  • Storage recommendations
    -20°C
  • Shipping recommendations
    Blue Ice or 4°C
  • Certificate of analysis
    A 1:1000 dilution of SPC-620 was sufficient for detection of GABARAP on mouse kidney lysates using Goat anti-rabbit IgG:HRP as the secondary antibody.
  • Antibody in cell
    Endomembrane System, Cytoplasm, Cytoskeleton, Golgi Apparatus, Golgi Apparatus Membrane, Cytoplasmic Vesicle
  • Tissue specificity
    See included datasheet or contact our support service.
  • Scientific context
    Gamma-aminobutyric acid receptor-associated protein, or GABARAP, are lighand gated chloride channels that mediate inhibitory neurotransmission. It clusters neurotransmitter receptors by mediating its interaction with the cytoskeleton (1).
  • Bibliography
    1. "Entrez Gene: GABARAP GABA(A) receptor-associated protein".
  • Released date
    1/Oct/2015
  • NCBI number
    NP_009209.1
  • Gene number
    11337
  • Protein number
    O95166
  • PubMed number
    Refer to PubMed
  • Tested applications
    To be tested
  • Tested reactivity
    To be tested
  • Antibody s datasheet
    Contact our support service to receive datasheet or other technical documentation.
  • Representative figure link
  • Representative figure legend
    Immunocytochemistry/Immunofluorescence analysis using Rabbit Anti-GABARAP Polyclonal Antibody (SPC-620). Tissue: Neuroblastoma cell line (SK-N-BE). Species: Human. Fixation: 4% Formaldehyde for 15 min at RT. Primary Antibody: Rabbit Anti-GABARAP Polyclonal Antibody (SPC-620) at 1:100 for 60 min at RT. Secondary Antibody: Goat Anti-Rabbit ATTO 488 at 1:100 for 60 min at RT. Counterstain: Phalloidin Texas Red F-Actin stain; DAPI (blue) nuclear stain at 1:1000, 1:5000 for 60min RT, 5min RT. Localization: Endomembrane System, Cytoplasm, Golgi Apparatus Membrane, Cytoplasmic Vesicle. Magnification: 60X. (A) DAPI (blue) nuclear stain (B) Phalloidin Texas Red F-Actin stain (C) GABARAP Antibody (D) Composite. | Western blot analysis of Human 293T showing detection of ~13.9kDa GABARAP protein using Rabbit Anti-GABARAP Polyclonal Antibody (SPC-620). Lane 1: MW Ladder. Lane 2: Human 293T (20 µg). Load: 20 µg. Block: 5% milk + TBST for 1 hour at RT. Primary Antibody: Rabbit Anti-GABARAP Polyclonal Antibody (SPC-620) at 1:1000 for 1 hour at RT. Secondary Antibody: Goat Anti-Rabbit: HRP at 1:2000 for 1 hour at RT. Color Development: TMB solution for 12 min at RT. Predicted/Observed Size: ~13.9kDa. Other Band(s): ~40kDa (Complex). | Western blot analysis of Mouse Kidney showing detection of ~13.9kDa GABARAP protein using Rabbit Anti-GABARAP Polyclonal Antibody (SPC-620). Lane 1: MW Ladder. Lane 2: Mouse Kidney (20 µg). Load: 20 µg. Block: 5% milk + TBST for 1 hour at RT. Primary Antibody: Rabbit Anti-GABARAP Polyclonal Antibody (SPC-620) at 1:1000 for 1 hour at RT. Secondary Antibody: Goat Anti-Rabbit: HRP at 1:2000 for 1 hour at RT. Color Development: TMB solution for 12 min at RT. Predicted/Observed Size: ~13.9kDa. Other Band(s): ~40kDa (Complex). Rabbit Anti-GABARAP Antibody used in Immunocytochemistry/Immunofluorescence (ICC/IF) on Neuroblastoma cell line (SK-N-BE) (SPC-620) | Rabbit Anti-GABARAP Antibody used in Western blot (WB) on 293T (SPC-620) | Rabbit Anti-GABARAP Antibody used in Western blot (WB) on Kidney (SPC-620)
  • Warning information
    Non-hazardous
  • Country of production
    Canada
  • Total weight kg
    1.4
  • Net weight g
    0.012
  • Stock availabilit
    In Stock
  • Description
    This antibody needs to be stored at + 4°C in a fridge short term in a concentrated dilution. Freeze thaw will destroy a percentage in every cycle and should be avoided. Antibody for research use.
  • Test
    StressMark antibodies supplies antibodies that are for research of human proteins.
  • Properties
    Human proteins, cDNA and human recombinants are used in human reactive ELISA kits and to produce anti-human mono and polyclonal antibodies. Modern humans (Homo sapiens, primarily ssp. Homo sapiens sapiens). Depending on the epitopes used human ELISA kits can be cross reactive to many other species. Mainly analyzed are human serum, plasma, urine, saliva, human cell culture supernatants and biological samples.
  • Group
    Polyclonals and antibodies
  • About
    Polyclonals can be used for Western blot, immunohistochemistry on frozen slices or parrafin fixed tissues. The advantage is that there are more epitopes available in a polyclonal antiserum to detect the proteins than in monoclonal sera. Rabbits are used for polyclonal antibody production by StressMark antibodies. Rabbit antibodies are very stable and can be stored for several days at room temperature. StressMark antibodies adds sodium azide and glycerol to enhance the stability of the rabbit polyclonal antibodies. Anti-human, anti mouse antibodies to highly immunogenic selected peptide sequences are" monoclonal like" since the epitope to which they are directed is less than 35 amino acids long.
  • Latin name
    Oryctolagus cuniculus
  • Conjugation
    not conjugated 
  • Gene target
  • Gene symbol
    GABARAP
  • Short name
    Rabbit Anti- GABARAP Polyclonal: Unconjugated
  • Technique
    anti-Human, Polyclonal, Rabbit, anti-, anti, antibody to, antibodies, antibodies against human proteins, Polyclonal antibodies (pAbs) are mostly rabbit or goat antibodies that are secreted by different B cells, whereas monoclonal antibodies come from a single N cell lineage. Pabs are a collection of immunoglobulin molecules that react against a specific antigen, each identifying a different epitope.
  • Host
    Rabbit, Rabbits
  • Isotype
    Immunoglobulin
  • Label
    Unconjugated
  • Species
    Human, Humans
  • Alternative name
    production species: rabbit Antibody toHuman GABARAP polyclonal: nonconjugated
  • Alternative technique
    polyclonals, rabbit-anti, antibodies
Gene info
MeSH Data
  • Name
  • Concept
    Scope note: Identification of proteins or peptides that have been electrophoretically separated by blot transferring from the electrophoresis gel to strips of nitrocellulose paper, followed by labeling with antibody probes.
  • Tree numbers
    • E05.196.401.143
    • E05.301.300.096
    • E05.478.566.320.200
    • E05.601.262
    • E05.601.470.320.200
  • Qualifiers
    ethics, trends, veterinary, history, classification, economics, instrumentation, methods, standards, statistics & numerical data
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