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Immunogen
A synthesized peptide derived from human FOXP3
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Antibody s target
Human FOXP3
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Antibody s full description
Rabbit Anti-Human FOXP3 Polyclonal
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Antibody s category
Polyclonal Antibodies
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Antibody s other name
AIID antibody, DIETER antibody, Forkhead box P3 antibody, Forkhead box protein P3 antibody, FOXP3 antibody, FOXP3_HUMAN antibody, FOXP3delta7 antibody, Immune dysregulation polyendocrinopathy enteropathy X linked antibody, Immunodeficiency polyendocrinopathy enteropathy X linked antibody, IPEX antibody, JM2 antibody, MGC141961 antibody, MGC141963 antibody, OTTHUMP00000025832 antibody, OTTHUMP00000025833 antibody, OTTHUMP00000226737 antibody, PIDX antibody, Scurfin antibody, XPID antibody
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Verified applications
WB, IHC, ICC/IF, IP
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Raised in
Rabbit
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Antibody s reactivity
Human
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Antibody s recommended dilutions for use
WB (1:2000), IHC (1:200), ICC/IF (1:500); optimal dilutions for assays should be determined by the user.
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Antibody s purified from
Peptide Affinity Purified
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Recommended buffer for storage
PBS pH 7.4, 50% glycerol, 150mM NaCl, 0.02% sodium azide
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Antibody s concentration
See included datasheet or contact our support service
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Antibody s specificity
Detects endogenous levels of total FOXP3
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Storage recommendations
-20°C
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Shipping recommendations
Blue Ice or 4°C
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Certificate of analysis
A 1:1000 dilution of SPC-1323 was sufficient for detection of FOXP3 in 10 µg of Jurkat cell lysates by ECL immunoblot analysis using Goat Anti-Rabbit IgG:HRP as the secondary antibody.
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Antibody in cell
Nucleus, Cytoplasm
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Tissue specificity
See included datasheet or contact our support service.
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Scientific context
FOXP3 (forkhead box P3), also known as scurfin, is a protein involved in immune system responses. A member of the FOX protein family, FOXP3 appears to function as a master regulator of the regulatory pathway in the development and function of regulatory T cells. FOXP3 is stably and constitutively expressed at a high level in CD25 + CD4 positive regulatory T cells, a low level in CD4-positive/CD25-negative cells, and is absent in CD4-negative/CD8-positive T cells. FOXP3 may be a master regulatory gene and a more specific marker of regulatory T cells than other T cells.
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Bibliography
1. Brunkow ME. et al. (2001) Nature Genetics 27 (1): 68–73. 2. Hori S. et al. (2003) Science 299 (5609): 1057–61. 3. Fontenot JD. et al. (2003) Nature Immunology 4 (4): 330–6. 4. Fontenot JD. et al. (2005) Immunity 22 (3): 329–41.
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Released date
6/Sep/2016
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NCBI number
NP_054728.2
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Gene number
50943
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Protein number
Q9BZS1
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PubMed number
Refer to PubMed
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Tested applications
To be tested
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Tested reactivity
To be tested
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Antibody s datasheet
Contact our support service to receive datasheet or other technical documentation.
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Representative figure link
No Data Available
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Warning information
Non-hazardous
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Country of production
Canada
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Total weight kg
1.4
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Net weight g
0.1
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Stock availabilit
In Stock
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Description
This antibody needs to be stored at + 4°C in a fridge short term in a concentrated dilution. Freeze thaw will destroy a percentage in every cycle and should be avoided. Antibody for research use.
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Test
StressMark antibodies supplies antibodies that are for research of human proteins.
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Gene
Forkhead Box Protein P3 scurfin FOXP3, is a protein involved in immune system responses. A member of the FOX protein family, FOXP3 appears to function as a master regulator of the regulatory pathway in the development and function of regulatory T cells. Foxp3 or forkhead box P3 or skurfin or scurfin or skurfina is also known as IPEX, AIID, PIDX, XPID and DIETER.
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Properties
HRP conjugates are often primary rabbit polyclonal antibodies couples to the enzyme horseradish peroxidase (HRP), found in the roots of horseradish. Also monoclonal HRP conjugates are often used for Western Blot. Human proteins, cDNA and human recombinants are used in human reactive ELISA kits and to produce anti-human mono and polyclonal antibodies. Modern humans (Homo sapiens, primarily ssp. Homo sapiens sapiens). Depending on the epitopes used human ELISA kits can be cross reactive to many other species. Mainly analyzed are human serum, plasma, urine, saliva, human cell culture supernatants and biological samples.
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Conjugation
HRP Conjugated
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Group
Polyclonals and antibodies
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About
Polyclonals can be used for Western blot, immunohistochemistry on frozen slices or parrafin fixed tissues. The advantage is that there are more epitopes available in a polyclonal antiserum to detect the proteins than in monoclonal sera. Rabbits are used for polyclonal antibody production by StressMark antibodies. Rabbit antibodies are very stable and can be stored for several days at room temperature. StressMark antibodies adds sodium azide and glycerol to enhance the stability of the rabbit polyclonal antibodies. Anti-human, anti mouse antibodies to highly immunogenic selected peptide sequences are" monoclonal like" since the epitope to which they are directed is less than 35 amino acids long.
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Latin name
Oryctolagus cuniculus
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Gene target
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Gene symbol
FOXP3
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Short name
Rabbit Anti- FOXP3 Polyclonal:
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Technique
anti-Human, Polyclonal, Rabbit, anti-, anti, antibody to, antibodies, antibodies against human proteins, Polyclonal antibodies (pAbs) are mostly rabbit or goat antibodies that are secreted by different B cells, whereas monoclonal antibodies come from a single N cell lineage. Pabs are a collection of immunoglobulin molecules that react against a specific antigen, each identifying a different epitope.
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Host
Rabbit, Rabbits
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Isotype
IgG
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Label
HRP
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Species
Human, Humans
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Alternative name
production species: rabbit Antibody toHuman forkhead box P3 polyclonal: horseradish peroxidase
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Alternative technique
polyclonals, rabbit-anti, antibodies
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Alternative to gene target
forkhead box P3, FOXP3 and IDBG-66215 and ENSG00000049768 and 50943, NFAT protein binding, nuclei, Foxp3 and IDBG-128986 and ENSMUSG00000039521 and 20371, FOXP3 and IDBG-637186 and ENSBTAG00000013279 and 506053
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