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Immunogen
A phospho-specific peptide corresponding to residues surrounding Thr185 and Tyr187 of human ERK2 (AA 182-188)
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Antibody s target
Human ERK2 (pThr185 + pTyr187)
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Antibody s full description
Rabbit Anti-Human ERK2 (pThr185 + pTyr187) Polyclonal
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Antibody s category
Polyclonal Antibodies
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Antibody s other name
ERK Antibody, ERT1 Antibody, ERK2 Antibody, Extracellular Signal Regulated Kinase 2 Antibody, MAP kinase 1 Antibody, MAP kinase 2 Antibody, MAP kinase isoform p42 Antibody, MAPK1 Antibody, MAPK2 Antibody, Mitogen-activated protein kinase 1 Antibody, Mitogen-activated protein kinase 2 Antibody, MK01_HUMAN Antibody, P38 Antibody, P40 Antibody, P41 Antibody, P42MAPK Antibody, PRKM1 Antibody, PRKM2 Antibody protein kinase, protein tyrosine kinase ERK2 Antibody
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Verified applications
WB, AM
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Raised in
Rabbit
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Antibody s reactivity
Human
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Antibody s recommended dilutions for use
WB (1:250); optimal dilutions for assays should be determined by the user.
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Antibody s purified from
Peptide Affinity Purified
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Recommended buffer for storage
PBS pH7.4, 50% glycerol, 0.025% Thimerosal
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Antibody s concentration
See included datasheet or contact our support service
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Antibody s specificity
Detects 41.39 kDa.
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Storage recommendations
-20°C
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Shipping recommendations
Blue Ice or 4°C
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Certificate of analysis
A 1:250 dilution of SPC-975 was sufficient for detection of ERK2 (pThr185 + pTyr187) in 10 µg of HeLa cell lysate by ECL immunoblot analysis using goat anti-rabbit IgG:HRP as the secondary antibody.
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Antibody in cell
Cytoplasm, Cytoskeleton, Spindle, Nucleus
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Tissue specificity
See included datasheet or contact our support service.
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Scientific context
ERK2 or MAPK1 is a protein-serine/threonine kinase. Phosphorylates many diffrent transcription factors, such as ELK1. Acts as transcriptional repressor by binding directly to DNA. Essential for cyclin D1 induction. MAPK1 phosphoryltaes BCL2, which contributes to cell survival, the suppression of the apoptotic effect of BAD and up-regulation of the antiapoptotic protein MCL-1. Regulates accumulation of p53 during DNA damage response. Constitutively active in many human tumours, supposedly due to altered RAS, RAF, EGFR or other upstream elements. ERK/MAPK pathway was shown to promote cell motilty and tumour cell migration.
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Bibliography
Refer to PubMed
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Released date
29/Aug/2016
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NCBI number
NP_002736.3
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Gene number
5594
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Protein number
P28482
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PubMed number
Refer to PubMed
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Tested applications
To be tested
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Tested reactivity
To be tested
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Antibody s datasheet
Contact our support service to receive datasheet or other technical documentation.
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Representative figure link
No Data Available
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Warning information
Non-hazardous
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Country of production
Canada
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Total weight kg
1.4
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Net weight g
0.1
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Stock availabilit
In Stock
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Description
This antibody needs to be stored at + 4°C in a fridge short term in a concentrated dilution. Freeze thaw will destroy a percentage in every cycle and should be avoided. Antibody for research use.
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Test
StressMark antibodies supplies antibodies that are for research of human proteins.
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Properties
This StressMark antibodies Fluorescein isothiocyanate (FITC) antibody is currently after some BD antibodies the most commonly used fluorescent dye for FACS. When excited at 488 nanometers, FITC has a green emission that's usually collected at 530 nanometers, the FL1 detector of a FACSCalibur or FACScan. FITC has a high quantum yield (efficiency of energy transfer from absorption to emission fluorescence) and approximately half of the absorbed photons are emitted as fluorescent light. For fluorescent microscopy applications, the 1 FITC is seldom used as it photo bleaches rather quickly though in flow cytometry applications, its photo bleaching effects are not observed due to a very brief interaction at the laser intercept. StressMark antibodies FITC is highly sensitive to pH extremes. Human proteins, cDNA and human recombinants are used in human reactive ELISA kits and to produce anti-human mono and polyclonal antibodies. Modern humans (Homo sapiens, primarily ssp. Homo sapiens sapiens). Depending on the epitopes used human ELISA kits can be cross reactive to many other species. Mainly analyzed are human serum, plasma, urine, saliva, human cell culture supernatants and biological samples.
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Conjugation
Anti-FITC Antibody
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Group
Polyclonals and antibodies
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About
Polyclonals can be used for Western blot, immunohistochemistry on frozen slices or parrafin fixed tissues. The advantage is that there are more epitopes available in a polyclonal antiserum to detect the proteins than in monoclonal sera. Rabbits are used for polyclonal antibody production by StressMark antibodies. Rabbit antibodies are very stable and can be stored for several days at room temperature. StressMark antibodies adds sodium azide and glycerol to enhance the stability of the rabbit polyclonal antibodies. Anti-human, anti mouse antibodies to highly immunogenic selected peptide sequences are" monoclonal like" since the epitope to which they are directed is less than 35 amino acids long.
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Latin name
Oryctolagus cuniculus
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Gene target
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Gene symbol
MAPK1
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Short name
Rabbit Anti- ERK2 (pThr185 + pTyr187) Polyclonal: FITC
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Technique
anti-Human, Polyclonal, Rabbit, anti-, anti, FITC, antibody to, antibodies, antibodies against human proteins, Fluorescein, Polyclonal antibodies (pAbs) are mostly rabbit or goat antibodies that are secreted by different B cells, whereas monoclonal antibodies come from a single N cell lineage. Pabs are a collection of immunoglobulin molecules that react against a specific antigen, each identifying a different epitope.
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Host
Rabbit, Rabbits
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Isotype
Immunoglobulin
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Label
FITC
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Species
Human, Humans
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Alternative name
production species: rabbit Antibody toHuman ERK2 (pThr185 + pTyr187) polyclonal: fluorecein
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Alternative technique
polyclonals, rabbit-anti, antibodies, fluorescine
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