Rabbit Anti-Human Ki67 Polyclonal: FITC

  • Catalog number
    SPC-1327D-FITC
  • Price
    Please ask
  • Size
    100 µl
  • Immunogen
    Synthesized peptide derived from C-terminal of human ki67.
  • Antibody s target
    Human Ki67
  • Antibody s full description
    Rabbit Anti-Human Ki67 Polyclonal
  • Antibody s category
    Polyclonal Antibodies
  • Antibody s other name
    Antigen identified by monoclonal antibody Ki 67 antibody, Antigen KI-67 antibody, Antigen KI67 antibody, KI67_HUMAN antibody, KIA antibody, Marker of proliferation Ki-67 antibody, MIB 1 antibody, MIB antibody, MKI67 antibody, PPP1R105 antibody, Proliferation related Ki 67 antigen antibody, Protein phosphatase 1 regulatory subunit 105 antibody, RP11-380J17.2 antibody
  • Verified applications
    IHC, ICC/IF, ELISA
  • Raised in
    Rabbit
  • Antibody s reactivity
    Human
  • Antibody s recommended dilutions for use
    IHC (1:100), ICC/IF (1:500), ELISA (1:20000); optimal dilutions for assays should be determined by the user.
  • Antibody s purified from
    Peptide Affinity Purified
  • Recommended buffer for storage
    PBS pH 7.4, 50% glycerol, 150mM NaCl, 0.02% sodium azide
  • Antibody s concentration
    See included datasheet or contact our support service
  • Antibody s specificity
    Detects endogenous levels of total ki67protein.
  • Storage recommendations
    -20°C
  • Shipping recommendations
    Blue Ice or 4°C
  • Certificate of analysis
    A 1:100 dilution of SPC-1327 was sufficient for detection of Ki67 in human lung carcinoa tissue using Goat Anti-Rabbit IgG as the secondary antibody.
  • Antibody in cell
    Nucleus, Chromosome
  • Tissue specificity
    See included datasheet or contact our support service.
  • Scientific context
    Antigen KI-67 also known as Ki-67 or MKI67 is a protein that in humans is encoded by the MKI67 gene. Antigen KI-67 is a nuclear protein that is associated with and may be necessary for cellular proliferation. Furthermore, it is associated with ribosomal RNA transcription. Inactivation of antigen KI-67 leads to inhibition of ribosomal RNA synthesis. Due to its association with cell divison process, Ki-67 is routinely used as cellular proliferation marker of solid tumors as well as certain hematological malignancies, and a correlation has been demonstrated between Ki-67 index and the histopathological grade of cancers.
  • Bibliography
    1. Schonk DM. et al. (1989) Hum. Genet. 83 (3): 297–9. 2. Bullwinkel J. et al. (2006) J. Cell. Physiol. 206 (3): 624–35. 3. Rahmanzadeh R. et al. (2007) Cell Prolif. 40 (3): 422–30. 4. Scholzen T and Gerdes J (2000) J. Cell. Physiol. 182 (3): 311–22.
  • Released date
    6/Sep/2016
  • NCBI number
    NP_002408.3
  • Gene number
    4288
  • Protein number
    P46013
  • PubMed number
    Refer to PubMed
  • Tested applications
    To be tested
  • Tested reactivity
    To be tested
  • Antibody s datasheet
    Contact our support service to receive datasheet or other technical documentation.
  • Representative figure link
  • Representative figure legend
    Immunohistochemistry analysis using Rabbit Anti-Ki67 Polyclonal Antibody (SPC-1327). Tissue: Lung Carcinoma Tissue. Species: Human. Fixation: Formalin fixed paraffin-embedded. Primary Antibody: Rabbit Anti-Ki67 Polyclonal Antibody (SPC-1327) at 1:100. The image on the right is treated with the synthesized peptide. | Immunocytochemistry/Immunofluorescence analysis using Rabbit Anti-Ki67 Polyclonal Antibody (SPC-1327). Tissue: Cos7. Species: Monkey. Primary Antibody: Rabbit Anti-Ki67 Polyclonal Antibody (SPC-1327) at 1:100. The image on the right is treated with the synthesized peptide. Rabbit Anti-Ki67 Antibody used in Immunohistochemistry (IHC) on Lung Carcinoma Tissue (SPC-1327) | Rabbit Anti-Ki67 Antibody used in Immunocytochemistry/Immunofluorescence (ICC/IF) on Cos7 (SPC-1327)
  • Warning information
    Non-hazardous
  • Country of production
    Canada
  • Total weight kg
    1.4
  • Net weight g
    0.1
  • Stock availabilit
    In Stock
  • Description
    This antibody needs to be stored at + 4°C in a fridge short term in a concentrated dilution. Freeze thaw will destroy a percentage in every cycle and should be avoided. Antibody for research use.
  • Test
    StressMark antibodies supplies antibodies that are for research of human proteins.
  • Properties
    This StressMark antibodies Fluorescein isothiocyanate (FITC) antibody is currently after some BD antibodies the most commonly used fluorescent dye for FACS. When excited at 488 nanometers, FITC has a green emission that's usually collected at 530 nanometers, the FL1 detector of a FACSCalibur or FACScan. FITC has a high quantum yield (efficiency of energy transfer from absorption to emission fluorescence) and approximately half of the absorbed photons are emitted as fluorescent light. For fluorescent microscopy applications, the 1 FITC is seldom used as it photo bleaches rather quickly though in flow cytometry applications, its photo bleaching effects are not observed due to a very brief interaction at the laser intercept. StressMark antibodies FITC is highly sensitive to pH extremes. Human proteins, cDNA and human recombinants are used in human reactive ELISA kits and to produce anti-human mono and polyclonal antibodies. Modern humans (Homo sapiens, primarily ssp. Homo sapiens sapiens). Depending on the epitopes used human ELISA kits can be cross reactive to many other species. Mainly analyzed are human serum, plasma, urine, saliva, human cell culture supernatants and biological samples.
  • Conjugation
    Anti-FITC Antibody
  • Group
    Polyclonals and antibodies
  • About
    Polyclonals can be used for Western blot, immunohistochemistry on frozen slices or parrafin fixed tissues. The advantage is that there are more epitopes available in a polyclonal antiserum to detect the proteins than in monoclonal sera. Rabbits are used for polyclonal antibody production by StressMark antibodies. Rabbit antibodies are very stable and can be stored for several days at room temperature. StressMark antibodies adds sodium azide and glycerol to enhance the stability of the rabbit polyclonal antibodies. Anti-human, anti mouse antibodies to highly immunogenic selected peptide sequences are" monoclonal like" since the epitope to which they are directed is less than 35 amino acids long.
  • Latin name
    Oryctolagus cuniculus
  • Gene target
    Ki67  
  • Short name
    Rabbit Anti- Ki67 Polyclonal: FITC
  • Technique
    anti-Human, Polyclonal, Rabbit, anti-, anti, FITC, antibody to, antibodies, antibodies against human proteins, Fluorescein, Polyclonal antibodies (pAbs) are mostly rabbit or goat antibodies that are secreted by different B cells, whereas monoclonal antibodies come from a single N cell lineage. Pabs are a collection of immunoglobulin molecules that react against a specific antigen, each identifying a different epitope.
  • Host
    Rabbit, Rabbits
  • Isotype
    IgG
  • Label
    FITC
  • Species
    Human, Humans
  • Alternative name
    production species: rabbit Antibody toHuman Ki67 polyclonal: fluorecein
  • Alternative technique
    polyclonals, rabbit-anti, antibodies, fluorescine
MeSH Data
  • Name
  • Concept
    Scope note: A method for the study of certain organic compounds within cells, in situ, by measuring the light intensities of the selectively stained areas of cytoplasm. The compounds studied and their locations in the cells are made to fluoresce and are observed under a microscope.
  • Tree numbers
    • E01.370.225.500.386
    • E05.196.712.516.600.240
    • E05.200.500.386
    • E05.242.386
  • Qualifiers
    ethics, trends, veterinary, history, classification, economics, instrumentation, methods, standards, statistics & numerical data
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