Rabbit Anti-Human EML4 (pTyr226) Polyclonal: ATTO 565

  • Catalog number
    SPC-964D-A565
  • Price
    Please ask
  • Size
    100 µl
  • Immunogen
    A phospho-specific peptide corresponding to residues surrounding Tyr226 of human EML4 (AA223-229)
  • Antibody s target
    Human EML4 (pTyr226)
  • Antibody s full description
    Rabbit Anti-Human EML4 (pTyr226) Polyclonal
  • Antibody s category
    Polyclonal Antibodies
  • Antibody s other name
    C2orf2 Antibody, Echinoderm microtubule associated protein like 4 Antibody, ELP 120 Antibody, EMAL4_HUMAN Antibody, EMAP4 Antibody, Restrictedly overexpressed proliferation associated protein Antibody, ROPP120 Antibody
  • Verified applications
    WB, AM
  • Raised in
    Rabbit
  • Antibody s reactivity
    Human
  • Antibody s recommended dilutions for use
    WB (1:250); optimal dilutions for assays should be determined by the user.
  • Antibody s purified from
    Peptide Affinity Purified
  • Recommended buffer for storage
    PBS pH7.4, 50% glycerol, 0.025% Thimerosal
  • Antibody s concentration
    See included datasheet or contact our support service
  • Antibody s specificity
    Detects 108.903 kDa.
  • Storage recommendations
    -20°C
  • Shipping recommendations
    Blue Ice or 4°C
  • Certificate of analysis
    A 1:250 dilution of SPC-964 was sufficient for detection of EML4 (pTyr226) in 10 µg of HeLa cell lysate by ECL immunoblot analysis using goat anti-rabbit IgG:HRP as the secondary antibody.
  • Antibody in cell
    Cytoskeleton
  • Tissue specificity
    See included datasheet or contact our support service.
  • Scientific context
    Echinoderm microtubule-associated (EML) proteins function to modify the assembly dynamics of microtubules. EML4 (Echinoderm microtubule-associated protein-like 4), also known as EMAPL4, ELP120, C2orf2 or ROPP120, is a 981 amino acid cytoplasmic protein that contains nine WD repeats. Expressed at high levels during mitosis, EML4 is thought to modify the assembly dynamics of microtubules, specifically altering microtubules to become longer and more flexible. Due to a chromosomal inversion with chromosome 2p, EML4 may exist as a fusion protein with ALK (anaplastic lymphoma receptor tyrosine kinase), producing an EML4-ALK fusion complex that plays a role in the pathogenesis of lung cancer.
  • Bibliography
    Refer to PubMed
  • Released date
    29/Aug/2016
  • NCBI number
    NP_001138548
  • Gene number
    27436
  • Protein number
    Q9HC35
  • PubMed number
    Refer to PubMed
  • Tested applications
    To be tested
  • Tested reactivity
    To be tested
  • Antibody s datasheet
    Contact our support service to receive datasheet or other technical documentation.
  • Representative figure link
    No Data Available
  • Warning information
    Non-hazardous
  • Country of production
    Canada
  • Total weight kg
    1.4
  • Net weight g
    0.1
  • Stock availabilit
    In Stock
  • Description
    This antibody needs to be stored at + 4°C in a fridge short term in a concentrated dilution. Freeze thaw will destroy a percentage in every cycle and should be avoided. Antibody for research use.
  • Test
    StressMark antibodies supplies antibodies that are for research of human proteins.
  • Properties
    Human proteins, cDNA and human recombinants are used in human reactive ELISA kits and to produce anti-human mono and polyclonal antibodies. Modern humans (Homo sapiens, primarily ssp. Homo sapiens sapiens). Depending on the epitopes used human ELISA kits can be cross reactive to many other species. Mainly analyzed are human serum, plasma, urine, saliva, human cell culture supernatants and biological samples.
  • Group
    Polyclonals and antibodies
  • About
    Polyclonals can be used for Western blot, immunohistochemistry on frozen slices or parrafin fixed tissues. The advantage is that there are more epitopes available in a polyclonal antiserum to detect the proteins than in monoclonal sera. Rabbits are used for polyclonal antibody production by StressMark antibodies. Rabbit antibodies are very stable and can be stored for several days at room temperature. StressMark antibodies adds sodium azide and glycerol to enhance the stability of the rabbit polyclonal antibodies. Anti-human, anti mouse antibodies to highly immunogenic selected peptide sequences are" monoclonal like" since the epitope to which they are directed is less than 35 amino acids long.
  • Latin name
    Oryctolagus cuniculus
  • Gene target
    EML4   pTyr226   ATTO   565  
  • Gene symbol
    EML4-AS1, EML4
  • Short name
    Rabbit Anti- EML4 (pTyr226) Polyclonal: ATTO 565
  • Technique
    anti-Human, Polyclonal, Rabbit, anti-, anti, antibody to, antibodies, antibodies against human proteins, Polyclonal antibodies (pAbs) are mostly rabbit or goat antibodies that are secreted by different B cells, whereas monoclonal antibodies come from a single N cell lineage. Pabs are a collection of immunoglobulin molecules that react against a specific antigen, each identifying a different epitope.
  • Host
    Rabbit, Rabbits
  • Isotype
    Immunoglobulin
  • Label
    ATTO 565
  • Species
    Human, Humans
  • Alternative name
    production species: rabbit Antibody toHuman EML4 (pTyr226) polyclonal: ATTO 565
  • Alternative technique
    polyclonals, rabbit-anti, antibodies
Gene info
Gene info
MeSH Data
  • Name
  • Concept
    Scope note: Identification of proteins or peptides that have been electrophoretically separated by blot transferring from the electrophoresis gel to strips of nitrocellulose paper, followed by labeling with antibody probes.
  • Tree numbers
    • E05.196.401.143
    • E05.301.300.096
    • E05.478.566.320.200
    • E05.601.262
    • E05.601.470.320.200
  • Qualifiers
    ethics, trends, veterinary, history, classification, economics, instrumentation, methods, standards, statistics & numerical data
Product images
Similar products
Filters
Contact
Chat with gentaur.com employee