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Immunogen
Peptide sequence around aa.273~277(T-I-L-V-E)derived from Human DDX4.
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Antibody s target
Human DDX4
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Antibody s full description
Rabbit Anti-Human DDX4 Polyclonal
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Antibody s category
Polyclonal Antibodies
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Antibody s other name
DDX 4 antibody, Ddx4 antibody, DDX4_HUMAN antibody, DEAD (Asp Glu Ala Asp) box polypeptide 4 antibody, DEAD box protein 4 antibody, DEAD/H (Asp-Glu-Ala-Asp/His) box polypeptide 4 antibody, MVH antibody, Probable ATP dependent RNA helicase DDX4 antibody, Probable ATP-dependent RNA helicase DDX4 antibody, VASA antibody, Vasa homolog antibody
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Verified applications
WB
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Raised in
Rabbit
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Antibody s reactivity
Human, Mouse, Rat
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Antibody s recommended dilutions for use
WB (1:1000); optimal dilutions for assays should be determined by the user.
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Antibody s purified from
Peptide Affinity Purified
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Recommended buffer for storage
PBS pH 7.4, 50% glycerol, 150mM NaCl, 0.02% sodium azide
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Antibody s concentration
See included datasheet or contact our support service
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Antibody s specificity
Detects endogenous level of total DDX4 protein.
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Storage recommendations
-20°C
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Shipping recommendations
Blue Ice or 4°C
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Certificate of analysis
A 1:1000 dilution of SPC-1322 was sufficient for detection of DDX4 in 10 µg of HeLa, K562, 3T3 cell lysates by ECL immunoblot analysis using Goat Anti-Rabbit IgG:HRP as the secondary antibody.
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Antibody in cell
Cytoplasm, Perinuclear Region
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Tissue specificity
See included datasheet or contact our support service.
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Scientific context
DEAD box proteins, characterized by the conserved motif Asp-Glu-Ala-Asp (DEAD), are putative RNA helicases. They are implicated in a number of cellular processes involving alteration of RNA secondary structure such as translation initiation, nuclear and mitochondrial splicing, and ribosome and spliceosome assembly. Based on their distribution patterns, some members of this family are believed to be involved in embryogenesis, spermatogenesis, and cellular growth and division. This gene encodes a DEAD box protein, which is a homolog of VASA proteins in Drosophila and several other species. The gene is specifically expressed in the germ cell lineage in both sexes and functions in germ cell development.
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Bibliography
1. Castrillon D.H. et al. (2000) Proc. Natl. Acad. Sci. U.S.A. 97:9585-9590. 2. Schmutz J. et al. (2004) Nature 431:268-274.
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Released date
6/Sep/2016
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NCBI number
NP_077726.1
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Gene number
54514
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Protein number
Q9NQI0
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PubMed number
Refer to PubMed
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Tested applications
To be tested
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Tested reactivity
To be tested
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Antibody s datasheet
Contact our support service to receive datasheet or other technical documentation.
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Representative figure legend
Western blot analysis of Human, Mouse HeLa, K562, 3T3 cell lysates showing detection of ~80kDa DDX4 protein using Rabbit Anti-DDX4 Polyclonal Antibody (SPC-1322). Lane 1: Human HeLa. Lane 2: Human K562. Lane 3: Mouse 3T3. Primary Antibody: Rabbit Anti-DDX4 Polyclonal Antibody (SPC-1322) at 1:1000. Predicted/Observed Size: ~80kDa. Rabbit Anti-DDX4 Antibody used in Western blot (WB) on HeLa, K562, 3T3 cell lysates (SPC-1322)
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Warning information
Non-hazardous
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Country of production
Canada
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Total weight kg
1.4
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Net weight g
0.1
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Stock availabilit
In Stock
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Description
This antibody needs to be stored at + 4°C in a fridge short term in a concentrated dilution. Freeze thaw will destroy a percentage in every cycle and should be avoided. Antibody for research use.
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Test
StressMark antibodies supplies antibodies that are for research of human proteins.
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Properties
Human proteins, cDNA and human recombinants are used in human reactive ELISA kits and to produce anti-human mono and polyclonal antibodies. Modern humans (Homo sapiens, primarily ssp. Homo sapiens sapiens). Depending on the epitopes used human ELISA kits can be cross reactive to many other species. Mainly analyzed are human serum, plasma, urine, saliva, human cell culture supernatants and biological samples.
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Group
Polyclonals and antibodies
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About
Polyclonals can be used for Western blot, immunohistochemistry on frozen slices or parrafin fixed tissues. The advantage is that there are more epitopes available in a polyclonal antiserum to detect the proteins than in monoclonal sera. Rabbits are used for polyclonal antibody production by StressMark antibodies. Rabbit antibodies are very stable and can be stored for several days at room temperature. StressMark antibodies adds sodium azide and glycerol to enhance the stability of the rabbit polyclonal antibodies. Anti-human, anti mouse antibodies to highly immunogenic selected peptide sequences are" monoclonal like" since the epitope to which they are directed is less than 35 amino acids long.
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Latin name
Oryctolagus cuniculus
