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Immunogen
A phospho-specific peptide corresponding to residues surrounding Ser383 and Ser385 of human CrkRS (AA380-388)
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Antibody s target
Human CrkRS (pSer383 + pSer385)
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Antibody s full description
Rabbit Anti-Human CrkRS (pSer383 + pSer385) Polyclonal
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Antibody s category
Polyclonal Antibodies
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Antibody s other name
arginine/serine-rich Antibody, Cdc2 related kinase arginine/serine rich Antibody, CDC2 related protein kinase 7 Antibody, Cdk12 Antibody, Cell division cycle 2 related protein kinase 7 Antibody, Cell division protein kinase 12 Antibody, CRK7 Antibody, Pksc Antibody
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Verified applications
WB, AM
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Raised in
Rabbit
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Antibody s reactivity
Human
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Antibody s recommended dilutions for use
WB (1:250); optimal dilutions for assays should be determined by the user.
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Antibody s purified from
Peptide Affinity Purified
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Recommended buffer for storage
PBS pH7.4, 50% glycerol, 0.025% Thimerosal
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Antibody s concentration
See included datasheet or contact our support service
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Antibody s specificity
Detects 164.155 kDa.
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Storage recommendations
-20°C
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Shipping recommendations
Blue Ice or 4°C
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Certificate of analysis
A 1:250 dilution of SPC-941 was sufficient for detection of CrkRS (pSer383 + pSer385) in 10 µg of 293T cell lysate by ECL immunoblot analysis using goat anti-rabbit IgG:HRP as the secondary antibody.
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Antibody in cell
Nucleus
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Tissue specificity
See included datasheet or contact our support service.
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Scientific context
CrkRS (Cdc2-related kinase, arginine/serine-rich, also designated CRK7 and CRKR) is an ubiquitous protein that appears to localize to the nucleus and link transcription and splicing machinery. CrkRS belongs to the serine/threonine protein kinase family and Cdc2/Cdkx subfamily. CrkRS has extensive proline-rich regions that resemble SH3 and WW domain binding sites, and an RS domain that is characteristic of splicing factors. The protein kinase domain of CrkRS is 89% identical to the CHED protein kinase, also designated CDC2L5 and cell division cycle 2-like 5 (cholinesterase-related cell division controller), however outside the kinase domains the two proteins are unique. Cell cycle control kinases can phosphorylate proteins important for differentiation and apoptosis and provide connections between proliferation, differentiation, apoptosis, and neurocytoskeleton dynamics.
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Bibliography
Refer to PubMed
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Released date
29/Aug/2016
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NCBI number
NP_057591
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Gene number
51755
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Protein number
Q9NYV4
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PubMed number
Refer to PubMed
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Tested applications
To be tested
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Tested reactivity
To be tested
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Antibody s datasheet
Contact our support service to receive datasheet or other technical documentation.
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Representative figure link
No Data Available
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Warning information
Non-hazardous
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Country of production
Canada
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Total weight kg
1.4
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Net weight g
0.1
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Stock availabilit
In Stock
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Description
This antibody needs to be stored at + 4°C in a fridge short term in a concentrated dilution. Freeze thaw will destroy a percentage in every cycle and should be avoided. Antibody for research use.
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Test
StressMark antibodies supplies antibodies that are for research of human proteins.
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Properties
Human proteins, cDNA and human recombinants are used in human reactive ELISA kits and to produce anti-human mono and polyclonal antibodies. Modern humans (Homo sapiens, primarily ssp. Homo sapiens sapiens). Depending on the epitopes used human ELISA kits can be cross reactive to many other species. Mainly analyzed are human serum, plasma, urine, saliva, human cell culture supernatants and biological samples.
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Group
Polyclonals and antibodies
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About
Polyclonals can be used for Western blot, immunohistochemistry on frozen slices or parrafin fixed tissues. The advantage is that there are more epitopes available in a polyclonal antiserum to detect the proteins than in monoclonal sera. Rabbits are used for polyclonal antibody production by StressMark antibodies. Rabbit antibodies are very stable and can be stored for several days at room temperature. StressMark antibodies adds sodium azide and glycerol to enhance the stability of the rabbit polyclonal antibodies. Anti-human, anti mouse antibodies to highly immunogenic selected peptide sequences are" monoclonal like" since the epitope to which they are directed is less than 35 amino acids long.
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Latin name
Oryctolagus cuniculus
