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Immunogen
Synthesized peptide derived from human Cox2.
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Antibody s target
Human Cox 2
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Antibody s full description
Rabbit Anti-Human Cox 2 Polyclonal
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Antibody s category
Polyclonal Antibodies
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Antibody s other name
COX 2 antibody, COX3 antibody, Cyclooxygenase 1 antibody, PCOX 1 antibody, PGG/HS antibody, PGH synthase 2 antibody, PGHS 1 antibody, PHS II antibody, Prostaglandin endoperoxide synthase 1 antibody, Prostaglandin endoperoxide synthase 2 antibody, PTGS2 antibody, TIS10 antibody
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Verified applications
WB, IHC, ICC/IF, ELISA
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Raised in
Rabbit
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Antibody s reactivity
Human, Mouse, Rat
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Antibody s recommended dilutions for use
WB (1:1000), IHC (1:100), ICC/IF (1:500), ELISA (1:20000); optimal dilutions for assays should be determined by the user.
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Antibody s purified from
Peptide Affinity Purified
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Recommended buffer for storage
PBS pH 7.4, 50% glycerol, 150mM NaCl, 0.02% sodium azide
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Antibody s concentration
See included datasheet or contact our support service
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Antibody s specificity
Detects endogenous levels of total Cox2 protein.
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Storage recommendations
-20°C
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Shipping recommendations
Blue Ice or 4°C
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Certificate of analysis
A 1:1000 dilution of SPC-1321 was sufficient for detection of Cox2 in 10 µg of A549 cell lysates by ECL immunoblot analysis using Goat Anti-Rabbit IgG:HRP as the secondary antibody.
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Antibody in cell
Microsome membrane, Endoplasmic reticulum membrane
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Tissue specificity
See included datasheet or contact our support service.
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Scientific context
Cyclooxygenase (COX), also known as Prostaglandin H2 synthase and Prostaglandin endoperoxide synthase, is a key enzyme in the of conversion arachidonic acid to Prostaglandin H2. Prostaglandin H2 is converted by other enzymes into inflammatory mediators prostaglandin (PG) D2, PGE2, PGF2a, PGI2 and Thromboxane A2. Thus, COX is a key enzyme in the production of inflammatory agents and is the target of intense research and drug discovery activities. There are two enzymes of COX, COX 1 and COX 2. COX 2 is found in elevated levels in synoviocytes from rheumatoid arthritis patients. The discomforts of inflammation such as pain and swelling are largely due to the action of prostaglandins produced by COX-2. COX2 (Cyclooxygenase 2) is an inducible enzyme. It is involved in the response of cells to growth factors, tumor promoters, and cytokines that induce its expression. Given its role in synthesizing prostaglandins, COX2 is therefore of interest in studying immune response regulation. COX2 is induced by a wide variety of stimuli and was initially identified as immediate early growth response gene. In addition, COX2 expression markedly increased in 85-90% of human colorectal adenocarcinoma whereas COX1 levels remain unchanged.
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Bibliography
1. Jones D.A. et al. (1993) J. Biol. Chem. 268:9049-9054. 2. Kosaka T. et al. (1994) Eur. J. Biochem. 221:889-897. 3. Appleby S.B. et al. (1994) Biochem. J. 302:723-727. 4. Barnett J. et al. (1994) Biochim. Biophys. Acta 1209:130-139.
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Released date
6/Sep/2016
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NCBI number
NP_000954.1
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Gene number
5743
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Protein number
P35354
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PubMed number
Refer to PubMed
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Tested applications
To be tested
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Tested reactivity
To be tested
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Antibody s datasheet
Contact our support service to receive datasheet or other technical documentation.
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Representative figure link
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Representative figure legend
Immunohistochemistry analysis using Rabbit Anti-Cox2 Polyclonal Antibody (SPC-1321). Tissue: Breast Carcinoma Tissue. Species: Human. Fixation: Formalin fixed paraffin-embedded. Primary Antibody: Rabbit Anti-Cox2 Polyclonal Antibody (SPC-1321) at 1:100. The image on the right is treated with the synthesized peptide. | Immunocytochemistry/Immunofluorescence analysis using Rabbit Anti-Cox2 Polyclonal Antibody (SPC-1321). Tissue: A549. Species: Human. Primary Antibody: Rabbit Anti-Cox2 Polyclonal Antibody (SPC-1321) at 1:100. The image on the right is treated with the synthesized peptide. | Western blot analysis of Human A549 cell lysates showing detection of ~74kDa Cox2 protein using Rabbit Anti-Cox2 Polyclonal Antibody (SPC-1321). Lane 1: Human A549. Lane 2: Human A549 treated with the immunizing peptide. Primary Antibody: Rabbit Anti-Cox2 Polyclonal Antibody (SPC-1321) at 1:1000. Predicted/Observed Size: ~74kDa. Rabbit Anti-Cox2 Antibody used in Immunohistochemistry (IHC) on Breast Carcinoma Tissue (SPC-1321) | Rabbit Anti-Cox2 Antibody used in Immunocytochemistry/Immunofluorescence (ICC/IF) on A549 (SPC-1321) | Rabbit Anti-Cox2 Antibody used in Western blot (WB) on A549 cell lysates (SPC-1321)
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Warning information
Non-hazardous
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Country of production
Canada
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Total weight kg
1.4
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Net weight g
0.1
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Stock availabilit
In Stock
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Description
This antibody needs to be stored at + 4°C in a fridge short term in a concentrated dilution. Freeze thaw will destroy a percentage in every cycle and should be avoided. Antibody for research use.
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Test
StressMark antibodies supplies antibodies that are for research of human proteins.
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Properties
Human proteins, cDNA and human recombinants are used in human reactive ELISA kits and to produce anti-human mono and polyclonal antibodies. Modern humans (Homo sapiens, primarily ssp. Homo sapiens sapiens). Depending on the epitopes used human ELISA kits can be cross reactive to many other species. Mainly analyzed are human serum, plasma, urine, saliva, human cell culture supernatants and biological samples.
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Group
Polyclonals and antibodies
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About
Polyclonals can be used for Western blot, immunohistochemistry on frozen slices or parrafin fixed tissues. The advantage is that there are more epitopes available in a polyclonal antiserum to detect the proteins than in monoclonal sera. Rabbits are used for polyclonal antibody production by StressMark antibodies. Rabbit antibodies are very stable and can be stored for several days at room temperature. StressMark antibodies adds sodium azide and glycerol to enhance the stability of the rabbit polyclonal antibodies. Anti-human, anti mouse antibodies to highly immunogenic selected peptide sequences are" monoclonal like" since the epitope to which they are directed is less than 35 amino acids long.
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Latin name
Oryctolagus cuniculus
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Conjugation
not conjugated
