-
Immunogen
Synthesized peptide derived from N-terminal of human Collagen I.
-
Antibody s target
Human Collagen I
-
Antibody s full description
Rabbit Anti-Human Collagen I Polyclonal
-
Primary research fields
Cell Signaling, Extracellular Matrix (ECM), Collagen, Stem Cells, Mesenchymal Stem Cells, Osteogenesis
-
Antibody s category
Polyclonal Antibodies
-
Antibody s other name
Alpha 1 type I collagen Antibody, Alpha 2 type I collagen Antibody, CO1A1_HUMAN Antibody, collagen alpha 1 chain type I Antibody, EDSC Antibody, Type I procollagen antibody
-
Verified applications
IHC, ICC/IF, ELISA
-
Raised in
Rabbit
-
Antibody s reactivity
Human, Mouse, Rat
-
Antibody s recommended dilutions for use
IHC (1:100), ICC/IF (1:500), ELISA (1:5000); optimal dilutions for assays should be determined by the user.
-
Antibody s purified from
Peptide Affinity Purified
-
Recommended buffer for storage
PBS pH 7.4, 50% glycerol, 150mM NaCl, 0.02% sodium azide
-
Antibody s concentration
See included datasheet or contact our support service
-
Antibody s specificity
Detects ~139kDa, endogenous levels of total Collagen I protein. Collage type I is a triple helix consisting of one alpha-2 and 2 alpha-1 chains, so dimeric (~270kDa) and trimeric (~400kDa) forms may be expected.
-
Storage recommendations
-20°C
-
Shipping recommendations
Blue Ice or 4°C
-
Certificate of analysis
A 1:100 dilution of SPC-1273 was sufficient for detection of Collagen I in NIH/3T3 cells using Goat Anti-Rabbit IgG as the secondary antibody.
-
Antibody in cell
Extracellular matrix
-
Tissue specificity
Forms the fibrils of tendon, ligaments and bones. In bones the fibrils are mineralized with calcium hydroxyapatite.
-
Scientific context
Type-I collagen is the predominant form of collagen in the human body and plays a role in scar tissue formation. Collagen type 1 is also an important component of tendons and found in most connective tissues including cartilage. Collagen 1 is associated with several diseases including: Ehlers Danlos syndrome (arthrochalasia), Ehlers-Danlos syndrome (classical type), Osteogenesis imperfecta type 1-4 and osteoporosis.
-
Bibliography
1. Stuart J.M., et al. (2005) Arthritis Rheum. 26(7): 832-840.
-
Released date
6/Sep/2016
-
NCBI number
NP_000080.2
-
Gene number
1278
-
Protein number
P08123
-
PubMed number
Refer to PubMed
-
Tested applications
To be tested
-
Tested reactivity
To be tested
-
Antibody s datasheet
Contact our support service to receive datasheet or other technical documentation.
-
Representative figure link
-
Representative figure legend
Immunohistochemistry analysis using Rabbit Anti-Collagen I Polyclonal Antibody (SPC-1273). Tissue: Breast Carcinoma Tissue. Species: Human. Fixation: Formalin fixed paraffin-embedded. Primary Antibody: Rabbit Anti-Collagen I Polyclonal Antibody (SPC-1273) at 1:100. The image on the right is treated with the synthesized peptide. | Immunocytochemistry/Immunofluorescence analysis using Rabbit Anti-Collagen I Polyclonal Antibody (SPC-1273). Tissue: NIH/3T3. Species: Mouse. Primary Antibody: Rabbit Anti-Collagen I Polyclonal Antibody (SPC-1273) at 1:100. The image on the right is treated with the synthesized peptide. Rabbit Anti-Collagen I Antibody used in Immunohistochemistry (IHC) on Breast Carcinoma Tissue (SPC-1273) | Rabbit Anti-Collagen I Antibody used in Immunocytochemistry/Immunofluorescence (ICC/IF) on NIH/3T3 (SPC-1273)
-
Warning information
Non-hazardous
-
Country of production
Canada
-
Total weight kg
1.4
-
Net weight g
0.1
-
Stock availabilit
In Stock
-
-
Description
This antibody needs to be stored at + 4°C in a fridge short term in a concentrated dilution. Freeze thaw will destroy a percentage in every cycle and should be avoided. Antibody for research use.
-
Test
StressMark antibodies supplies antibodies that are for research of human proteins.
-
Gene
Collagen Types in bones and muscles can be hydrolyzed and used in human grafts. ( Type II )
-
Properties
Human proteins, cDNA and human recombinants are used in human reactive ELISA kits and to produce anti-human mono and polyclonal antibodies. Modern humans (Homo sapiens, primarily ssp. Homo sapiens sapiens). Depending on the epitopes used human ELISA kits can be cross reactive to many other species. Mainly analyzed are human serum, plasma, urine, saliva, human cell culture supernatants and biological samples.
-
Group
Polyclonals and antibodies
-
About
Polyclonals can be used for Western blot, immunohistochemistry on frozen slices or parrafin fixed tissues. The advantage is that there are more epitopes available in a polyclonal antiserum to detect the proteins than in monoclonal sera. Rabbits are used for polyclonal antibody production by StressMark antibodies. Rabbit antibodies are very stable and can be stored for several days at room temperature. StressMark antibodies adds sodium azide and glycerol to enhance the stability of the rabbit polyclonal antibodies. Anti-human, anti mouse antibodies to highly immunogenic selected peptide sequences are" monoclonal like" since the epitope to which they are directed is less than 35 amino acids long.
-
Latin name
Oryctolagus cuniculus
