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Immunogen
Synthetic peptide from the N-terminal to the mid-protein of human Choline O-Acetyltrasferase
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Antibody s target
Human Choline Acetyltransferase
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Antibody s full description
Rabbit Anti-Human Choline Acetyltransferase Polyclonal
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Primary research fields
Neuroscience, Neurotransmitter Receptors, Cell Signaling, Neurodegeneration, Alzheimer's Disease
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Antibody s category
Polyclonal Antibodies
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Antibody s other name
CHAT_Human Antibody, Acetyl CoA choline O Acetyltransferase Antibody, Choline Acetylase Antibody, CLAT_Human Antibody, CMS1A Antibody
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Verified applications
WB, ICC/IF
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Raised in
Rabbit
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Antibody s reactivity
Human, Mouse
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Antibody s recommended dilutions for use
WB (1:1000); ICC/IF (1:100); optimal dilutions for assays should be determined by the user.
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Antibody s purified from
Peptide Affinity Purified
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Recommended buffer for storage
PBS, 50% glycerol, 0.09% sodium azide
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Antibody s concentration
1 mg/ml
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Antibody s specificity
Predicted molecular weight at ~82.5kDa. Observed molecular weights between 68-70kDa.
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Storage recommendations
-20°C
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Shipping recommendations
Blue Ice or 4°C
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Certificate of analysis
A 1:1000 dilution of SPC-706 was sufficient for detection of Choline Acetyltransferase on mouse brain lysates using Goat anti-rabbit IgG:HRP as the secondary antibody.
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Antibody in cell
Cytoplasm, Cytosol, Mitochondrion, Nucleus
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Tissue specificity
See included datasheet or contact our support service.
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Scientific context
Acetylcholine (ACh) is a common neurotransmitter for motoneurons, preganglionic autonomic neurons, postganglionic parasympathetic neurons, a variety of brain regions and some emerging neuron-like stem cells. The metabolism of Ach is relatively simple, involving only two enzymes: choline acetyltransferase (ChAT) for synthesis and acetylcholinesterase (AChE) for degradation. Further, acetylcholine has little function in neurons other than neurotransmission and seems to be neuron specific. It seems that only cholinergic neurons have significant amounts of ChAT making anti-choline acetyltransferase a useful specific marker. ChAT is a valuable marker for diseases associated with decreased cholinergic function such as Schizophrenia, Alzheimer disease and Down syndrome (1-3).
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Bibliography
1. Houser C.R., Crawford G.D., Barber R.P., Salvaterra P.M., Vaughn J.E. (1983) Brain Research. 266(1): 97-119. 2. Karson C.N., Casanova M.F., Kleinman J.E.m Griffin W.S. (1993) Am J Psychiatry. 150: 454-459. 3. Baskins D.S., et al. (1999) Arch Neurol. 56: 1121-1123.
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Released date
1/Oct/2015
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NCBI number
NP_001136401.1
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Gene number
1103
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Protein number
P28329
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PubMed number
Refer to PubMed
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Tested applications
To be tested
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Tested reactivity
To be tested
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Antibody s datasheet
Contact our support service to receive datasheet or other technical documentation.
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Representative figure link
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Representative figure legend
Immunocytochemistry/Immunofluorescence analysis using Rabbit Anti-Choline Acetyltransferase Polyclonal Antibody (SPC-706). Tissue: Neuroblastoma cell line (SK-N-BE). Species: Human. Fixation: 4% Formaldehyde for 15 min at RT. Primary Antibody: Rabbit Anti-Choline Acetyltransferase Polyclonal Antibody (SPC-706) at 1:100 for 60 min at RT. Secondary Antibody: Goat Anti-Rabbit ATTO 488 at 1:100 for 60 min at RT. Counterstain: Phalloidin Texas Red F-Actin stain; DAPI (blue) nuclear stain at 1:1000, 1:5000 for 60min RT, 5min RT. Localization: Nucleus. Magnification: 60X. (A) DAPI (blue) nuclear stain (B) Phalloidin Texas Red F-Actin stain (C) Choline Acetyltransferase Antibody (D) Composite. | Western blot analysis of Mouse Brain showing detection of ~82.5kDa Choline Acetyltransferase protein using Rabbit Anti-Choline Acetyltransferase Polyclonal Antibody (SPC-706). Lane 1: MW Ladder. Lane 2: Mouse Brain (20 µg). Load: 20 µg. Block: 5% milk + TBST for 1 hour at RT. Primary Antibody: Rabbit Anti-Choline Acetyltransferase Polyclonal Antibody (SPC-706) at 1:1000 for 1 hour at RT. Secondary Antibody: Goat Anti-Rabbit: HRP at 1:2000 for 1 hour at RT. Color Development: TMB solution for 12 min at RT. Predicted/Observed Size: ~82.5kDa. Rabbit Anti-Choline Acetyltransferase Antibody used in Immunocytochemistry/Immunofluorescence (ICC/IF) on Neuroblastoma cell line (SK-N-BE) (SPC-706) | Rabbit Anti-Choline Acetyltransferase Antibody used in Western blot (WB) on Brain (SPC-706)
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Warning information
Non-hazardous
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Country of production
Canada
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Total weight kg
1.4
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Net weight g
0.1
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Stock availabilit
In Stock
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Description
This antibody needs to be stored at + 4°C in a fridge short term in a concentrated dilution. Freeze thaw will destroy a percentage in every cycle and should be avoided. Antibody for research use.
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Test
StressMark antibodies supplies antibodies that are for research of human proteins.
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Properties
Human proteins, cDNA and human recombinants are used in human reactive ELISA kits and to produce anti-human mono and polyclonal antibodies. Modern humans (Homo sapiens, primarily ssp. Homo sapiens sapiens). Depending on the epitopes used human ELISA kits can be cross reactive to many other species. Mainly analyzed are human serum, plasma, urine, saliva, human cell culture supernatants and biological samples.
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Group
Polyclonals and antibodies
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About
Polyclonals can be used for Western blot, immunohistochemistry on frozen slices or parrafin fixed tissues. The advantage is that there are more epitopes available in a polyclonal antiserum to detect the proteins than in monoclonal sera. Rabbits are used for polyclonal antibody production by StressMark antibodies. Rabbit antibodies are very stable and can be stored for several days at room temperature. StressMark antibodies adds sodium azide and glycerol to enhance the stability of the rabbit polyclonal antibodies. Anti-human, anti mouse antibodies to highly immunogenic selected peptide sequences are" monoclonal like" since the epitope to which they are directed is less than 35 amino acids long.
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Latin name
Oryctolagus cuniculus
