Rabbit Anti-Human CDC25C Polyclonal: ATTO 594

  • Catalog number
    SPC-1114D-A594
  • Price
    Please ask
  • Size
    100 µl
  • Immunogen
    Synthetic peptide of human CDC25C (AA458-472)
  • Antibody s target
    Human CDC25C
  • Antibody s full description
    Rabbit Anti-Human CDC25C Polyclonal
  • Antibody s category
    Polyclonal Antibodies
  • Antibody s other name
    CDC 25 antibody, Cdc 25C antibody, CDC25 antibody, CDC25C antibody, Cell division cycle 25 homolog C antibody, Cell division cycle 25C antibody, Dual specificity phosphatase Cdc25C antibody, M phase inducer phosphatase 3 antibody, Mitosis inducer CDC25 antibody, MPIP3 antibody, MPIP3_HUMAN antibody, Phosphotyrosine phosphatase antibody, PPP1R60 antibody
  • Verified applications
    WB, AM
  • Raised in
    Rabbit
  • Antibody s reactivity
    Human, Mouse
  • Antibody s recommended dilutions for use
    WB (1:250); optimal dilutions for assays should be determined by the user.
  • Antibody s purified from
    Peptide Affinity Purified
  • Recommended buffer for storage
    PBS pH7.4, 50% glycerol, 0.025% Thimerosal
  • Antibody s concentration
    See included datasheet or contact our support service
  • Antibody s specificity
    Detects 53.365 kDa.
  • Storage recommendations
    -20°C
  • Shipping recommendations
    Blue Ice or 4°C
  • Certificate of analysis
    A 1:250 dilution of SPC-1114 was sufficient for detection of CDC25C in 10 µg of HeLa cell lysate by ECL immunoblot analysis using goat anti-rabbit IgG:HRP as the secondary antibody.
  • Antibody in cell
    Nucleus
  • Tissue specificity
    Expressed predominantly in G2 phase.
  • Scientific context
    The Cdc2/cyclin B enzyme, involved in regulation of mitosis in eukaryotic cells, is subject to multiple levels of control. Among these, the regulation of the catalytic subunit by Tyrosine phosphorylation is the best understood. Tyrosine phosphorylation inhibits the Cdc2/cyclin B complex, while Tyrosine dephosphorylation, which occurs at the onset of mitosis, directly activates the pre-MPH complex. The Cdc25 gene serves as a rate-limiting mitotic activator, apparently due to its action as the Cdc2 Tyrosine phosphatase. In the absence of Cdc25, Cdc2 accumulates in a Tyrosine phosphorylated state. In addition, Cdc25 proteins from a variety of species have been shown to share a low degree of sequence similarity with other Tyrosine phosphatases. The Cdc25 gene family consists of at least three members that share approximately 40% identity in their most conserved carboxy-terminal sequences.
  • Bibliography
    Refer to PubMed
  • Released date
    29/Aug/2016
  • NCBI number
    NP_001781
  • Gene number
    995
  • Protein number
    P30307
  • PubMed number
    Refer to PubMed
  • Tested applications
    To be tested
  • Tested reactivity
    To be tested
  • Antibody s datasheet
    Contact our support service to receive datasheet or other technical documentation.
  • Representative figure link
    No Data Available
  • Warning information
    Non-hazardous
  • Country of production
    Canada
  • Total weight kg
    1.4
  • Net weight g
    0.1
  • Stock availabilit
    In Stock
  • Description
    This antibody needs to be stored at + 4°C in a fridge short term in a concentrated dilution. Freeze thaw will destroy a percentage in every cycle and should be avoided. Antibody for research use.
  • Test
    StressMark antibodies supplies antibodies that are for research of human proteins.
  • Properties
    Human proteins, cDNA and human recombinants are used in human reactive ELISA kits and to produce anti-human mono and polyclonal antibodies. Modern humans (Homo sapiens, primarily ssp. Homo sapiens sapiens). Depending on the epitopes used human ELISA kits can be cross reactive to many other species. Mainly analyzed are human serum, plasma, urine, saliva, human cell culture supernatants and biological samples.
  • Group
    Polyclonals and antibodies
  • About
    Polyclonals can be used for Western blot, immunohistochemistry on frozen slices or parrafin fixed tissues. The advantage is that there are more epitopes available in a polyclonal antiserum to detect the proteins than in monoclonal sera. Rabbits are used for polyclonal antibody production by StressMark antibodies. Rabbit antibodies are very stable and can be stored for several days at room temperature. StressMark antibodies adds sodium azide and glycerol to enhance the stability of the rabbit polyclonal antibodies. Anti-human, anti mouse antibodies to highly immunogenic selected peptide sequences are" monoclonal like" since the epitope to which they are directed is less than 35 amino acids long.
  • Latin name
    Oryctolagus cuniculus
  • Gene target
    CDC25C   ATTO   594  
  • Gene symbol
    CDC25C
  • Short name
    Rabbit Anti- CDC25C Polyclonal: ATTO 594
  • Technique
    anti-Human, Polyclonal, Rabbit, anti-, anti, antibody to, antibodies, antibodies against human proteins, Polyclonal antibodies (pAbs) are mostly rabbit or goat antibodies that are secreted by different B cells, whereas monoclonal antibodies come from a single N cell lineage. Pabs are a collection of immunoglobulin molecules that react against a specific antigen, each identifying a different epitope.
  • Host
    Rabbit, Rabbits
  • Isotype
    Immunoglobulin
  • Label
    ATTO 594
  • Species
    Human, Humans
  • Alternative name
    production species: rabbit Antibody toHuman CDC25C polyclonal: ATTO 594
  • Alternative technique
    polyclonals, rabbit-anti, antibodies
Gene info
MeSH Data
  • Name
  • Concept
    Scope note: Identification of proteins or peptides that have been electrophoretically separated by blot transferring from the electrophoresis gel to strips of nitrocellulose paper, followed by labeling with antibody probes.
  • Tree numbers
    • E05.196.401.143
    • E05.301.300.096
    • E05.478.566.320.200
    • E05.601.262
    • E05.601.470.320.200
  • Qualifiers
    ethics, trends, veterinary, history, classification, economics, instrumentation, methods, standards, statistics & numerical data
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