Rabbit Anti-Human Aurora A (pThr287 + pThr288) Polyclonal: Streptavidin

  • Catalog number
    SPC-919D-STR
  • Price
    Please ask
  • Size
    100 µl
  • Immunogen
    A phospho-specific peptide corresponding to residues surrounding Thr287 and Thr288 of human Aurora A (AA285-291)
  • Antibody s target
    Human Aurora A (pThr287 + pThr288)
  • Antibody s full description
    Rabbit Anti-Human Aurora A (pThr287 + pThr288) Polyclonal
  • Antibody s category
    Polyclonal Antibodies
  • Antibody s other name
    Aurora 2 Antibody, AIK Antibody, ARK1 Antibody, BTAK Antibody
  • Verified applications
    WB, AM
  • Raised in
    Rabbit
  • Antibody s reactivity
    Human
  • Antibody s recommended dilutions for use
    WB (1:250); optimal dilutions for assays should be determined by the user.
  • Antibody s purified from
    Peptide Affinity Purified
  • Recommended buffer for storage
    PBS pH7.4, 50% glycerol, 0.025% Thimerosal
  • Antibody s concentration
    See included datasheet or contact our support service
  • Antibody s specificity
    Detects ~48kDa.
  • Storage recommendations
    -20°C
  • Shipping recommendations
    Blue Ice or 4°C
  • Certificate of analysis
    A 1:250 dilution of SPC-919 was sufficient for detection of Aurora A (pThr287 + pThr288) in 10 µg of HeLa cell lysate by ECL immunoblot analysis using goat anti-rabbit IgG:HRP as the secondary antibody.
  • Antibody in cell
    Cytoplasm, Cytoskeleton
  • Tissue specificity
    Highly expressed in testis and weakly in skeletal muscle, thymus and spleen. Also highly expressed in colon, ovarian, prostate, neuroblastoma, breast and cervical cancer cell lines.
  • Scientific context
    Aurora A is part of the larger AURA family of serine/threonine kinases and is a key regulator of mitotic cell division. It is involved in regulating microtubule formation and the assembly of the mitotic spindle responsible for chromosome segragation during mitosis. AURA activity is cell cycle dependent with the greatest activity occuring during mitosis. The overexpression of AURA has been well documented in many malignant cancers including breast, ovarian, colon, prostate and neuroblastomas. Overexpression of AURA appears to override checkpoints in cell cycle progression leading to increased cell division and proliferation.
  • Bibliography
    Refer to PubMed
  • Released date
    29/Aug/2016
  • NCBI number
    NP_003591
  • Gene number
    6790
  • Protein number
    O14965
  • PubMed number
    Refer to PubMed
  • Tested applications
    To be tested
  • Tested reactivity
    To be tested
  • Antibody s datasheet
    Contact our support service to receive datasheet or other technical documentation.
  • Representative figure link
    No Data Available
  • Warning information
    Non-hazardous
  • Country of production
    Canada
  • Total weight kg
    1.4
  • Net weight g
    0.1
  • Stock availabilit
    In Stock
  • Description
    This antibody needs to be stored at + 4°C in a fridge short term in a concentrated dilution. Freeze thaw will destroy a percentage in every cycle and should be avoided. Antibody for research use.
  • Test
    StressMark antibodies supplies antibodies that are for research of human proteins.
  • Properties
    Human proteins, cDNA and human recombinants are used in human reactive ELISA kits and to produce anti-human mono and polyclonal antibodies. Modern humans (Homo sapiens, primarily ssp. Homo sapiens sapiens). Depending on the epitopes used human ELISA kits can be cross reactive to many other species. Mainly analyzed are human serum, plasma, urine, saliva, human cell culture supernatants and biological samples.
  • Group
    Polyclonals and antibodies
  • About
    Polyclonals can be used for Western blot, immunohistochemistry on frozen slices or parrafin fixed tissues. The advantage is that there are more epitopes available in a polyclonal antiserum to detect the proteins than in monoclonal sera. Rabbits are used for polyclonal antibody production by StressMark antibodies. Rabbit antibodies are very stable and can be stored for several days at room temperature. StressMark antibodies adds sodium azide and glycerol to enhance the stability of the rabbit polyclonal antibodies. Anti-human, anti mouse antibodies to highly immunogenic selected peptide sequences are" monoclonal like" since the epitope to which they are directed is less than 35 amino acids long.
  • Latin name
    Oryctolagus cuniculus
  • Gene target
  • Short name
    Rabbit Anti- Aurora A (pThr287 + pThr288) Polyclonal: Streptavidin
  • Technique
    anti-Human, Polyclonal, Rabbit, anti-, anti, antibody to, antibodies, antibodies against human proteins, Polyclonal antibodies (pAbs) are mostly rabbit or goat antibodies that are secreted by different B cells, whereas monoclonal antibodies come from a single N cell lineage. Pabs are a collection of immunoglobulin molecules that react against a specific antigen, each identifying a different epitope.
  • Host
    Rabbit, Rabbits
  • Isotype
    Immunoglobulin
  • Label
    Streptavidin
  • Species
    Human, Humans
  • Alternative name
    production species: rabbit Antibody toHuman Aurora A (pThr287 + pThr288) polyclonal: biotinilated-binding streptavidin
  • Alternative technique
    polyclonals, rabbit-anti, antibodies
MeSH Data
  • Name
  • Concept
    Scope note: Identification of proteins or peptides that have been electrophoretically separated by blot transferring from the electrophoresis gel to strips of nitrocellulose paper, followed by labeling with antibody probes.
  • Tree numbers
    • E05.196.401.143
    • E05.301.300.096
    • E05.478.566.320.200
    • E05.601.262
    • E05.601.470.320.200
  • Qualifiers
    ethics, trends, veterinary, history, classification, economics, instrumentation, methods, standards, statistics & numerical data
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