Rabbit Anti-Human ATG5 Polyclonal: HRP

  • Catalog number
    SPC-612D-HRP
  • Price
    Please ask
  • Size
    100 µg
  • Immunogen
    Synthetic peptide from the N-terminal of human ATG5
  • Antibody s target
    Human ATG5
  • Antibody s full description
    Rabbit Anti-Human ATG5 Polyclonal
  • Primary research fields
    Cancer, Apoptosis, Cancer, Autophagy, Cardiovascular System, Heart, Cell Signaling, Metabolism, Metabolism processes
  • Antibody s category
    Polyclonal Antibodies
  • Antibody s other name
    APG5-like Antibody, ASP Antibody, hAPG5 Antibody
  • Verified applications
    WB, ICC/IF
  • Raised in
    Rabbit
  • Antibody s reactivity
    Human
  • Antibody s recommended dilutions for use
    WB (1:1000); ICC/IF (1:100); optimal dilutions for assays should be determined by the user.
  • Antibody s purified from
    Peptide Affinity Purified
  • Recommended buffer for storage
    PBS, 50% glycerol, 0.09% sodium azide
  • Antibody s concentration
    1 mg/ml
  • Antibody s specificity
    Predicted molecular weight at ~32.4kDa. Observed molecular weights at ~48-60kDa based on ATG12-ATG5 heterodimer.
  • Storage recommendations
    -20°C
  • Shipping recommendations
    Blue Ice or 4°C
  • Certificate of analysis
    A 1:1000 dilution of SPC-612 was sufficient for detection of ATG5 on HeLa cell lysates using Goat anti-rabbit IgG:HRP as the secondary antibody.
  • Antibody in cell
    Cytoplasm
  • Tissue specificity
    See included datasheet or contact our support service.
  • Scientific context
    ATG5 is required for autophagy. It conjugates to ATG12 and associates with isolation membrane to form autophagosomes. The conjugate detaches from the membrane immediately before or after autophagosome formation is completed (1). Conjugation to ATG12 is essential for autophagy, but is not required for association with isolation membrane. ATG5 also plays an important role in the apoptotic process. Its expression is a relatively late event in the apoptotic process, occurring downstream of caspase activity. ATG5 is activated by ATG7 and forms a complex with ATG12 and ATG16L1. This complex is necessary for LC3-1 conjugation to PE to form LC3-II (2).
  • Bibliography
    1. Pyo J.O., et al. (2005) J Biol Chem. 280(21): 20722-20729. 2. Mizushima N., et al. (2003) Mol Biol Cell. 15(3): 1101-1111.
  • Released date
    1/Oct/2015
  • NCBI number
    NP_001273035.1
  • Gene number
    9474
  • Protein number
     Q9H1Y0
  • PubMed number
    Refer to PubMed
  • Tested applications
    To be tested
  • Tested reactivity
    To be tested
  • Antibody s datasheet
    Contact our support service to receive datasheet or other technical documentation.
  • Representative figure link
  • Representative figure legend
    Immunocytochemistry/Immunofluorescence analysis using Rabbit Anti-ATG5 Polyclonal Antibody (SPC-612). Tissue: Cervical Cancer cell line (HeLa). Species: Human. Fixation: 4% Formaldehyde for 15 min at RT. Primary Antibody: Rabbit Anti-ATG5 Polyclonal Antibody (SPC-612) at 1:100 for 60 min at RT. Secondary Antibody: Goat Anti-Rabbit ATTO 488 at 1:100 for 60 min at RT. Counterstain: DAPI (blue) nuclear stain at 1:5000 for 5 min RT. Localization: Nucleus. Magnification: 40X. (A) DAPI (blue) nuclear stain (B) Phalloidin Texas Red F-Actin stain (C) ATG5 Antibody (D) Composite. | Western blot analysis of Human HeLa cell lysates showing detection of ~32.4kDa ATG5 protein using Rabbit Anti-ATG5 Polyclonal Antibody (SPC-612). Lane 1: MW Ladder. Lane 2: Human HeLa (20 µg). Load: 20 µg. Block: 5% milk + TBST for 1 hour at RT. Primary Antibody: Rabbit Anti-ATG5 Polyclonal Antibody (SPC-612) at 1:1000 for 1 hour at RT. Secondary Antibody: Goat Anti-Rabbit: HRP at 1:2000 for 1 hour at RT. Color Development: TMB solution for 12 min at RT. Predicted/Observed Size: ~32.4kDa. Other Band(s): ~105kDa. Rabbit Anti-ATG5 Antibody used in Immunocytochemistry/Immunofluorescence (ICC/IF) on Cervical Cancer cell line (HeLa) (SPC-612) | Rabbit Anti-ATG5 Antibody used in Western blot (WB) on HeLa cell lysates (SPC-612)
  • Warning information
    Non-hazardous
  • Country of production
    Canada
  • Total weight kg
    1.4
  • Net weight g
    0.1
  • Stock availabilit
    In Stock
  • Description
    This antibody needs to be stored at + 4°C in a fridge short term in a concentrated dilution. Freeze thaw will destroy a percentage in every cycle and should be avoided. Antibody for research use.
  • Test
    StressMark antibodies supplies antibodies that are for research of human proteins.
  • Properties
    HRP conjugates are often primary rabbit polyclonal antibodies couples to the enzyme horseradish peroxidase (HRP), found in the roots of horseradish. Also monoclonal HRP conjugates are often used for Western Blot. Human proteins, cDNA and human recombinants are used in human reactive ELISA kits and to produce anti-human mono and polyclonal antibodies. Modern humans (Homo sapiens, primarily ssp. Homo sapiens sapiens). Depending on the epitopes used human ELISA kits can be cross reactive to many other species. Mainly analyzed are human serum, plasma, urine, saliva, human cell culture supernatants and biological samples.
  • Conjugation
    HRP Conjugated
  • Group
    Polyclonals and antibodies
  • About
    Polyclonals can be used for Western blot, immunohistochemistry on frozen slices or parrafin fixed tissues. The advantage is that there are more epitopes available in a polyclonal antiserum to detect the proteins than in monoclonal sera. Rabbits are used for polyclonal antibody production by StressMark antibodies. Rabbit antibodies are very stable and can be stored for several days at room temperature. StressMark antibodies adds sodium azide and glycerol to enhance the stability of the rabbit polyclonal antibodies. Anti-human, anti mouse antibodies to highly immunogenic selected peptide sequences are" monoclonal like" since the epitope to which they are directed is less than 35 amino acids long.
  • Latin name
    Oryctolagus cuniculus
  • Gene target
    ATG5  
  • Gene symbol
    ATG5
  • Short name
    Rabbit Anti- ATG5 Polyclonal:
  • Technique
    anti-Human, Polyclonal, Rabbit, anti-, anti, antibody to, antibodies, antibodies against human proteins, Polyclonal antibodies (pAbs) are mostly rabbit or goat antibodies that are secreted by different B cells, whereas monoclonal antibodies come from a single N cell lineage. Pabs are a collection of immunoglobulin molecules that react against a specific antigen, each identifying a different epitope.
  • Host
    Rabbit, Rabbits
  • Isotype
    Immunoglobulin
  • Label
    HRP
  • Species
    Human, Humans
  • Alternative name
    production species: rabbit Antibody toHuman autophagy related 5 polyclonal: horseradish peroxidase
  • Alternative technique
    polyclonals, rabbit-anti, antibodies
  • Alternative to gene target
    autophagy related 5, APG5 and APG5-LIKE and APG5L and ASP and hAPG5, ATG5 and IDBG-94856 and ENSG00000057663 and 9474, protein binding, Plasma membranes, Atg5 and IDBG-148568 and ENSMUSG00000038160 and 11793, ATG5 and IDBG-631895 and ENSBTAG00000005400 and 532686
Gene info
  • Identity
  • Gene
  • Long gene name
    autophagy related 5
  • Synonyms gene
  • Synonyms gene name
    • APG5 (autophagy 5, S. cerevisiae)-like
    • APG5 autophagy 5-like (S. cerevisiae)
    • ATG5 autophagy related 5 homolog (S. cerevisiae)
  • Synonyms
  • GenBank acession
  • Locus
  • Discovery year
    1999-09-16
  • Entrez gene record
  • Pubmed identfication
  • RefSeq identity
  • Classification
    • Autophagy related
  • VEGA ID
MeSH Data
  • Name
  • Concept
    Scope note: Identification of proteins or peptides that have been electrophoretically separated by blot transferring from the electrophoresis gel to strips of nitrocellulose paper, followed by labeling with antibody probes.
  • Tree numbers
    • E05.196.401.143
    • E05.301.300.096
    • E05.478.566.320.200
    • E05.601.262
    • E05.601.470.320.200
  • Qualifiers
    ethics, trends, veterinary, history, classification, economics, instrumentation, methods, standards, statistics & numerical data
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