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Immunogen
Synthetic peptide from the N-terminal of human ATG5
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Antibody s target
Human ATG5
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Antibody s full description
Rabbit Anti-Human ATG5 Polyclonal
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Primary research fields
Cancer, Apoptosis, Cancer, Autophagy, Cardiovascular System, Heart, Cell Signaling, Metabolism, Metabolism processes
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Antibody s category
Polyclonal Antibodies
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Antibody s other name
APG5-like Antibody, ASP Antibody, hAPG5 Antibody
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Verified applications
WB, ICC/IF
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Raised in
Rabbit
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Antibody s reactivity
Human
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Antibody s recommended dilutions for use
WB (1:1000); ICC/IF (1:100); optimal dilutions for assays should be determined by the user.
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Antibody s purified from
Peptide Affinity Purified
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Recommended buffer for storage
PBS, 50% glycerol, 0.09% sodium azide
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Antibody s concentration
1 mg/ml
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Antibody s specificity
Predicted molecular weight at ~32.4kDa. Observed molecular weights at ~48-60kDa based on ATG12-ATG5 heterodimer.
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Storage recommendations
-20°C
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Shipping recommendations
Blue Ice or 4°C
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Certificate of analysis
A 1:1000 dilution of SPC-612 was sufficient for detection of ATG5 on HeLa cell lysates using Goat anti-rabbit IgG:HRP as the secondary antibody.
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Antibody in cell
Cytoplasm
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Tissue specificity
See included datasheet or contact our support service.
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Scientific context
ATG5 is required for autophagy. It conjugates to ATG12 and associates with isolation membrane to form autophagosomes. The conjugate detaches from the membrane immediately before or after autophagosome formation is completed (1). Conjugation to ATG12 is essential for autophagy, but is not required for association with isolation membrane. ATG5 also plays an important role in the apoptotic process. Its expression is a relatively late event in the apoptotic process, occurring downstream of caspase activity. ATG5 is activated by ATG7 and forms a complex with ATG12 and ATG16L1. This complex is necessary for LC3-1 conjugation to PE to form LC3-II (2).
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Bibliography
1. Pyo J.O., et al. (2005) J Biol Chem. 280(21): 20722-20729. 2. Mizushima N., et al. (2003) Mol Biol Cell. 15(3): 1101-1111.
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Released date
1/Oct/2015
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NCBI number
NP_001273035.1
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Gene number
9474
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Protein number
Q9H1Y0
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PubMed number
Refer to PubMed
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Tested applications
To be tested
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Tested reactivity
To be tested
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Antibody s datasheet
Contact our support service to receive datasheet or other technical documentation.
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Representative figure link
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Representative figure legend
Immunocytochemistry/Immunofluorescence analysis using Rabbit Anti-ATG5 Polyclonal Antibody (SPC-612). Tissue: Cervical Cancer cell line (HeLa). Species: Human. Fixation: 4% Formaldehyde for 15 min at RT. Primary Antibody: Rabbit Anti-ATG5 Polyclonal Antibody (SPC-612) at 1:100 for 60 min at RT. Secondary Antibody: Goat Anti-Rabbit ATTO 488 at 1:100 for 60 min at RT. Counterstain: DAPI (blue) nuclear stain at 1:5000 for 5 min RT. Localization: Nucleus. Magnification: 40X. (A) DAPI (blue) nuclear stain (B) Phalloidin Texas Red F-Actin stain (C) ATG5 Antibody (D) Composite. | Western blot analysis of Human HeLa cell lysates showing detection of ~32.4kDa ATG5 protein using Rabbit Anti-ATG5 Polyclonal Antibody (SPC-612). Lane 1: MW Ladder. Lane 2: Human HeLa (20 µg). Load: 20 µg. Block: 5% milk + TBST for 1 hour at RT. Primary Antibody: Rabbit Anti-ATG5 Polyclonal Antibody (SPC-612) at 1:1000 for 1 hour at RT. Secondary Antibody: Goat Anti-Rabbit: HRP at 1:2000 for 1 hour at RT. Color Development: TMB solution for 12 min at RT. Predicted/Observed Size: ~32.4kDa. Other Band(s): ~105kDa. Rabbit Anti-ATG5 Antibody used in Immunocytochemistry/Immunofluorescence (ICC/IF) on Cervical Cancer cell line (HeLa) (SPC-612) | Rabbit Anti-ATG5 Antibody used in Western blot (WB) on HeLa cell lysates (SPC-612)
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Warning information
Non-hazardous
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Country of production
Canada
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Total weight kg
1.4
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Net weight g
0.1
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Stock availabilit
In Stock
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Description
This antibody needs to be stored at + 4°C in a fridge short term in a concentrated dilution. Freeze thaw will destroy a percentage in every cycle and should be avoided. Antibody for research use.
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Test
StressMark antibodies supplies antibodies that are for research of human proteins.
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Properties
HRP conjugates are often primary rabbit polyclonal antibodies couples to the enzyme horseradish peroxidase (HRP), found in the roots of horseradish. Also monoclonal HRP conjugates are often used for Western Blot. Human proteins, cDNA and human recombinants are used in human reactive ELISA kits and to produce anti-human mono and polyclonal antibodies. Modern humans (Homo sapiens, primarily ssp. Homo sapiens sapiens). Depending on the epitopes used human ELISA kits can be cross reactive to many other species. Mainly analyzed are human serum, plasma, urine, saliva, human cell culture supernatants and biological samples.
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Conjugation
HRP Conjugated
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Group
Polyclonals and antibodies
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About
Polyclonals can be used for Western blot, immunohistochemistry on frozen slices or parrafin fixed tissues. The advantage is that there are more epitopes available in a polyclonal antiserum to detect the proteins than in monoclonal sera. Rabbits are used for polyclonal antibody production by StressMark antibodies. Rabbit antibodies are very stable and can be stored for several days at room temperature. StressMark antibodies adds sodium azide and glycerol to enhance the stability of the rabbit polyclonal antibodies. Anti-human, anti mouse antibodies to highly immunogenic selected peptide sequences are" monoclonal like" since the epitope to which they are directed is less than 35 amino acids long.
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Latin name
Oryctolagus cuniculus
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Gene target
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Gene symbol
ATG5
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Short name
Rabbit Anti- ATG5 Polyclonal:
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Technique
anti-Human, Polyclonal, Rabbit, anti-, anti, antibody to, antibodies, antibodies against human proteins, Polyclonal antibodies (pAbs) are mostly rabbit or goat antibodies that are secreted by different B cells, whereas monoclonal antibodies come from a single N cell lineage. Pabs are a collection of immunoglobulin molecules that react against a specific antigen, each identifying a different epitope.
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Host
Rabbit, Rabbits
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Isotype
Immunoglobulin
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Label
HRP
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Species
Human, Humans
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Alternative name
production species: rabbit Antibody toHuman autophagy related 5 polyclonal: horseradish peroxidase
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Alternative technique
polyclonals, rabbit-anti, antibodies
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Alternative to gene target
autophagy related 5, APG5 and APG5-LIKE and APG5L and ASP and hAPG5, ATG5 and IDBG-94856 and ENSG00000057663 and 9474, protein binding, Plasma membranes, Atg5 and IDBG-148568 and ENSMUSG00000038160 and 11793, ATG5 and IDBG-631895 and ENSBTAG00000005400 and 532686
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