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Immunogen
Synthetic peptide from the N-terminal of Human ATG4A (aa. 58-70)
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Antibody s target
Human ATG4A
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Antibody s full description
Rabbit Anti-Human ATG4A Polyclonal
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Primary research fields
Cancer, Autophagy, Cardiovascular System, Heart, Cell Signaling
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Antibody s category
Polyclonal Antibodies
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Antibody s other name
ATG4 autophagy related 4 homolog A (S. cerevisiae) Antibody, MGC107179 Antibody, AV169859 Antibody, Autophagy-related cysteine endopeptidase 2 Antibody, hAPG4A Antibody, EC:3.4.22.- Antibody, ATG4A Antibody, Autophagin 2 Antibody, Autophagy related protein 4 homolog A Antibody, Cysteine protease ATG4A Antibody, AUTL2 Antibody, Autl2 Antibody, Autophagy-related protein 4 homolog A Antibody, Atg4al Antibody, Autophagin-2 Antibody, Autophagy related 4A cysteine peptidase Antibody, AUT like 2 cysteine endopeptidase Antibody, Autophagy related cysteine endopeptidase 2 Antibody, Apg4a Antibody, AUT-like 2 cysteine endopeptidase Antibody, APG4A Antibody, AI627006 Antibody, ATG4A_HUMAN Antibody,
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Verified applications
WB, ICC/IF
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Raised in
Rabbit
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Antibody s reactivity
Human
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Antibody s recommended dilutions for use
WB (1:1000), ICC/IF (1:100); optimal dilutions for assays should be determined by the user.
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Antibody s purified from
Peptide Affinity Purified
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Recommended buffer for storage
PBS, 50% glycerol, 0.09% sodium azide
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Antibody s concentration
1 mg/ml
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Antibody s specificity
Detects ~48 kDa.
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Storage recommendations
-20°C
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Shipping recommendations
Blue Ice or 4°C
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Certificate of analysis
A 1:1000 dilution of SPC-629 was sufficient for detection of ATG4A in 15 µg of Human HeLa Cell Lysates by ECL immunoblot analysis using goat anti-rabbit IgG:HRP as the secondary antibody.
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Antibody in cell
Cytoplasm
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Tissue specificity
Widely expressed, at a low level, and the highest expression is observed in skeletal muscle and brain. Also detected in fetal liver.
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Scientific context
See included datasheet or contact our support service.
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Bibliography
Refer to PubMed
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Released date
29/Aug/2016
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NCBI number
NP_001308216.1
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Gene number
115201
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Protein number
Q8WYN0
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PubMed number
Refer to PubMed
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Tested applications
To be tested
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Tested reactivity
To be tested
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Antibody s datasheet
Contact our support service to receive datasheet or other technical documentation.
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Representative figure link
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Representative figure legend
Immunocytochemistry/Immunofluorescence analysis using Rabbit Anti-ATG4A Polyclonal Antibody (SPC-629). Tissue: SK-N-BE Cells (Human Neuroblastoma cells). Species: Human. Fixation: 4% Formaldehyde for 15 min at RT. Primary Antibody: Rabbit Anti-ATG4A Polyclonal Antibody (SPC-629) at 1:100 for 60 min at RT. Secondary Antibody: Goat Anti-Mouse ATTO 488 at 1:200 for 60 min at RT. Counterstain: Phalloidin Texas Red F-Actin stain; DAPI (blue) nuclear stain at 1:1000, 1:5000 for 60 min at RT, 5 min at RT. Localization: Cytoplasm. Magnification: 60X. (A) DAPI (blue) nuclear stain (B) Phalloidin Texas Red F-Actin stain (C) ATG4A Antibody (D) Composite. | Western blot analysis of Human HeLa cell lysates showing detection of 45.3 kda ATG4A protein using Rabbit Anti-ATG4A Polyclonal Antibody (SPC-629). Lane 1: Molecular Weight Ladder (MW). Lane 2: Human HeLa cell lysates. Load: 15 µg . Block: 5% Skim Milk in 1X TBST. Primary Antibody: Rabbit Anti-ATG4A Polyclonal Antibody (SPC-629) at 1:1000 for 1 hour at RT. Secondary Antibody: Goat Anti-Rabbit HRP at 1:2000 for 60 min at RT. Color Development: ECL solution for 6 min in RT. Predicted/Observed Size: 45.3 kda. Rabbit Anti-ATG4A Antibody used in Immunocytochemistry/Immunofluorescence (ICC/IF) on Human SK-N-BE Cells (Human Neuroblastoma cells) (SPC-629) | Rabbit Anti-ATG4A Antibody used in Western blot (WB) on HeLa cell lysates (SPC-629)
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Warning information
Non-hazardous
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Country of production
Canada
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Total weight kg
1.4
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Net weight g
0.1
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Stock availabilit
In Stock
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Description
This antibody needs to be stored at + 4°C in a fridge short term in a concentrated dilution. Freeze thaw will destroy a percentage in every cycle and should be avoided. Antibody for research use.
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Test
StressMark antibodies supplies antibodies that are for research of human proteins.
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Properties
Human proteins, cDNA and human recombinants are used in human reactive ELISA kits and to produce anti-human mono and polyclonal antibodies. Modern humans (Homo sapiens, primarily ssp. Homo sapiens sapiens). Depending on the epitopes used human ELISA kits can be cross reactive to many other species. Mainly analyzed are human serum, plasma, urine, saliva, human cell culture supernatants and biological samples.
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Group
Polyclonals and antibodies
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About
Polyclonals can be used for Western blot, immunohistochemistry on frozen slices or parrafin fixed tissues. The advantage is that there are more epitopes available in a polyclonal antiserum to detect the proteins than in monoclonal sera. Rabbits are used for polyclonal antibody production by StressMark antibodies. Rabbit antibodies are very stable and can be stored for several days at room temperature. StressMark antibodies adds sodium azide and glycerol to enhance the stability of the rabbit polyclonal antibodies. Anti-human, anti mouse antibodies to highly immunogenic selected peptide sequences are" monoclonal like" since the epitope to which they are directed is less than 35 amino acids long.
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Latin name
Oryctolagus cuniculus
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Gene target
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Gene symbol
ATG4A, APC
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Short name
Rabbit Anti- ATG4A Polyclonal: APC
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Technique
anti-Human, Polyclonal, Rabbit, anti-, anti, antibody to, antibodies, antibodies against human proteins, Polyclonal antibodies (pAbs) are mostly rabbit or goat antibodies that are secreted by different B cells, whereas monoclonal antibodies come from a single N cell lineage. Pabs are a collection of immunoglobulin molecules that react against a specific antigen, each identifying a different epitope.
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Host
Rabbit, Rabbits
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Isotype
Immunoglobulin
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Label
APC
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Species
Human, Humans
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Alternative name
production species: rabbit Antibody toHuman ATG4A polyclonal: adenomatous polyposis coli
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Alternative technique
polyclonals, rabbit-anti, antibodies
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Alternative to gene target
adenomatous polyposis coli, BTPS2 and DP2 and DP2.5 and DP3 and GS and PPP1R46, APC and IDBG-37007 and ENSG00000134982 and 324, microtubule plus-end binding, nuclei, Apc and IDBG-134573 and ENSMUSG00000005871 and 11789, BT.25770 and IDBG-644937 and ENSBTAG00000018852 and 533233
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