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Immunogen
Synthetic peptide from the mid-protein range of human ATG13
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Antibody s target
Human ATG13
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Antibody s full description
Rabbit Anti-Human ATG13 Polyclonal
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Primary research fields
Cancer, Autophagy
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Antibody s category
Polyclonal Antibodies
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Antibody s other name
ATG13_Human Antibody, Autophagy related protein 13 Antibody, KIAA0652 Antibody
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Verified applications
WB, ICC/IF
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Raised in
Rabbit
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Antibody s reactivity
Human, Mouse
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Antibody s recommended dilutions for use
WB (1:1000); ICC/IF (1:100); optimal dilutions for assays should be determined by the user.
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Antibody s purified from
Peptide Affinity Purified
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Recommended buffer for storage
PBS, 50% glycerol, 0.09% sodium azide
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Antibody s concentration
1 mg/ml
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Antibody s specificity
Detects ~56-63kDa.
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Storage recommendations
-20°C
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Shipping recommendations
Blue Ice or 4°C
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Certificate of analysis
A 1:1000 dilution of SPC-657 was sufficient for detection of ATG13 on HEK293 lysates using Goat anti-rabbit IgG:HRP as the secondary antibody.
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Antibody in cell
Cytoplasm, Cytosol, Preautophagosomal structure
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Tissue specificity
See included datasheet or contact our support service.
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Scientific context
ATG13 is an autophagy factor required for phagosome formation. It is a target of the TOR kinase signaling pathway that regulates autophagy through phosphorylation of ATG13 and ULK1, and the regulation of the ATG13-ULK1-RB1CC1 complex (1-3).
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Bibliography
1. Mercer C.A., Kaliappan A., Dennis P.B. (2009) Autophagy. 5(5): 649-662. 2. Hosokawa N., et al. (2009) Mol Biol Cell. 20(7): 1981-1991. 3. Chan E.Y., Longatti A., McKnight N.C., Tooze S.A. (2009) Mol Cell Biol. 29(1): 157-171.
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Released date
1/Oct/2015
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NCBI number
NP_001136145.1
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Gene number
9776
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Protein number
O75143
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PubMed number
Refer to PubMed
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Tested applications
To be tested
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Tested reactivity
To be tested
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Antibody s datasheet
Contact our support service to receive datasheet or other technical documentation.
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Representative figure link
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Representative figure legend
Immunocytochemistry/Immunofluorescence analysis using Rabbit Anti-ATG13 Polyclonal Antibody (SPC-657). Tissue: Myoblast cell line (C2C12). Species: Mouse. Fixation: 4% Formaldehyde for 15 min at RT. Primary Antibody: Rabbit Anti-ATG13 Polyclonal Antibody (SPC-657) at 1:100 for 60 min at RT. Secondary Antibody: Goat Anti-Rabbit ATTO 488 at 1:100 for 60 min at RT. Counterstain: DAPI (blue) nuclear stain at 1:5000 for 5 min RT. Localization: Cytoplasm, Cytosol. Magnification: 60X. (A) DAPI (blue) nuclear stain (B) Phalloidin Texas Red F-Actin stain (C) ATG13 Antibody (D) Composite. | Western blot analysis of Human HEK293 showing detection of ~56.6kDa ATG13 protein using Rabbit Anti-ATG13 Polyclonal Antibody (SPC-657). Lane 1: MW Ladder. Lane 2: Human HEK293 (20 µg). Load: 20 µg. Block: 5% milk + TBST for 1 hour at RT. Primary Antibody: Rabbit Anti-ATG13 Polyclonal Antibody (SPC-657) at 1:1000 for 1 hour at RT. Secondary Antibody: Goat Anti-Rabbit: HRP at 1:2000 for 1 hour at RT. Color Development: TMB solution for 12 min at RT. Predicted/Observed Size: ~56.6kDa. Rabbit Anti-ATG13 Antibody used in Immunocytochemistry/Immunofluorescence (ICC/IF) on Myoblast cell line (C2C12) (SPC-657) | Rabbit Anti-ATG13 Antibody used in Western blot (WB) on HEK293 (SPC-657)
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Warning information
Non-hazardous
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Country of production
Canada
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Total weight kg
1.4
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Net weight g
0.1
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Stock availabilit
In Stock
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Description
This antibody needs to be stored at + 4°C in a fridge short term in a concentrated dilution. Freeze thaw will destroy a percentage in every cycle and should be avoided. Antibody for research use.
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Test
StressMark antibodies supplies antibodies that are for research of human proteins.
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Properties
Human proteins, cDNA and human recombinants are used in human reactive ELISA kits and to produce anti-human mono and polyclonal antibodies. Modern humans (Homo sapiens, primarily ssp. Homo sapiens sapiens). Depending on the epitopes used human ELISA kits can be cross reactive to many other species. Mainly analyzed are human serum, plasma, urine, saliva, human cell culture supernatants and biological samples.
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Group
Polyclonals and antibodies
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About
Polyclonals can be used for Western blot, immunohistochemistry on frozen slices or parrafin fixed tissues. The advantage is that there are more epitopes available in a polyclonal antiserum to detect the proteins than in monoclonal sera. Rabbits are used for polyclonal antibody production by StressMark antibodies. Rabbit antibodies are very stable and can be stored for several days at room temperature. StressMark antibodies adds sodium azide and glycerol to enhance the stability of the rabbit polyclonal antibodies. Anti-human, anti mouse antibodies to highly immunogenic selected peptide sequences are" monoclonal like" since the epitope to which they are directed is less than 35 amino acids long.
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Latin name
Oryctolagus cuniculus
