-
Immunogen
Synthetic peptide from the mid-protein of Human ATG10
-
Antibody s target
Human ATG10
-
Antibody s full description
Rabbit Anti-Human ATG10 Polyclonal
-
Primary research fields
Neuroscience, Neurodegeneration, Cancer, Tumor suppressors, Cardiovascular System, Heart, Cancer, Autophagy, Cell Signaling, Metabolism, Metabolism processes
-
Antibody s category
Polyclonal Antibodies
-
Antibody s other name
APG 10L Antibody, APG10 like Antibody, Apg 10 Antibody, DKFZP586I0418 Antibody, ATG10 Antibody, ORF Names: Antibody, Ubiquitin-like-conjugating enzyme ATG10 Antibody, APG10, S. cerevisiae, homolog of Antibody, ATG10_HUMAN Antibody, ATG 10 Antibody, APG10-like Antibody, Autophagy related protein 10 Antibody, APG10 Antibody, Autophagy-related protein 10 Antibody, autophagy 10, S. cerevisiae, homolog of Antibody, APG10L Antibody, ATG10 autophagy related 10 homolog Antibody, ATG10 autophagy related 10 homolog (S. cerevisiae) Antibody, FLJ13954 Antibody, PP12616 Antibody, APG10 autophagy 10 like Antibody, pp12616 Antibody
-
Verified applications
WB
-
Raised in
Rabbit
-
Antibody s reactivity
Human
-
Antibody s recommended dilutions for use
WB (1:1000); optimal dilutions for assays should be determined by the user.
-
Antibody s purified from
Peptide Affinity Purified
-
Recommended buffer for storage
PBS, 50% glycerol, 0.09% sodium azide
-
Antibody s concentration
1 mg/ml
-
Antibody s specificity
Detects ~25 kDa.
-
Storage recommendations
-20°C
-
Shipping recommendations
Blue Ice or 4°C
-
Certificate of analysis
A 1:1000 dilution of SPC-669 was sufficient for detection of ATG10 in 15 µg of human HEK293T cell lysates by ECL immunoblot analysis using goat anti-rabbit IgG:HRP as the secondary antibody.
-
Antibody in cell
Cytoplasm
-
Scientific context
See included datasheet or contact our support service.
-
Bibliography
Refer to PubMed
-
Released date
25/Oct/2016
-
NCBI number
NP_001124500.1
-
Gene number
83734
-
Protein number
Q9H0Y0
-
PubMed number
Refer to PubMed
-
Tested applications
To be tested
-
Tested reactivity
To be tested
-
Antibody s datasheet
Contact our support service to receive datasheet or other technical documentation.
-
-
Representative figure legend
Western blot analysis of Human HEK293 cell lysates showing detection of ~25.3 kDa ATG10 protein using Rabbit Anti-ATG10 Polyclonal Antibody (SPC-669). Lane 1: Molecular Weight Ladder (MW). Lane 2: Hek293T cell lysates. Load: 15 µg. Block: 5% Skim Milk in 1X TBST. Primary Antibody: Rabbit Anti-ATG10 Polyclonal Antibody (SPC-669) at 1:1000 for 60 min at RT. Secondary Antibody: Goat Anti-Rabbit IgG: HRP at 1:2000 for 60 min at RT. Color Development: ECL solution for 6 min in RT. Predicted/Observed Size: ~25.3 kDa. Rabbit Anti-ATG10 Antibody used in Western blot (WB) on HEK293 cell lysates (SPC-669)
-
Warning information
Non-hazardous
-
Country of production
Canada
-
Total weight kg
1.4
-
Net weight g
0.1
-
Stock availabilit
In Stock
-
-
Description
This antibody needs to be stored at + 4°C in a fridge short term in a concentrated dilution. Freeze thaw will destroy a percentage in every cycle and should be avoided. Antibody for research use.
-
Test
StressMark antibodies supplies antibodies that are for research of human proteins.
-
Properties
Human proteins, cDNA and human recombinants are used in human reactive ELISA kits and to produce anti-human mono and polyclonal antibodies. Modern humans (Homo sapiens, primarily ssp. Homo sapiens sapiens). Depending on the epitopes used human ELISA kits can be cross reactive to many other species. Mainly analyzed are human serum, plasma, urine, saliva, human cell culture supernatants and biological samples.
-
Group
Polyclonals and antibodies
-
About
Polyclonals can be used for Western blot, immunohistochemistry on frozen slices or parrafin fixed tissues. The advantage is that there are more epitopes available in a polyclonal antiserum to detect the proteins than in monoclonal sera. Rabbits are used for polyclonal antibody production by StressMark antibodies. Rabbit antibodies are very stable and can be stored for several days at room temperature. StressMark antibodies adds sodium azide and glycerol to enhance the stability of the rabbit polyclonal antibodies. Anti-human, anti mouse antibodies to highly immunogenic selected peptide sequences are" monoclonal like" since the epitope to which they are directed is less than 35 amino acids long.
-
Latin name
Oryctolagus cuniculus
-
Conjugation
not conjugated
-
Gene target
-
Gene symbol
ATG10-AS1, ATG10-IT1, ATG10
-
Short name
Rabbit Anti- ATG10 Polyclonal: Unconjugated
-
Technique
anti-Human, Polyclonal, Rabbit, anti-, anti, antibody to, antibodies, antibodies against human proteins, Polyclonal antibodies (pAbs) are mostly rabbit or goat antibodies that are secreted by different B cells, whereas monoclonal antibodies come from a single N cell lineage. Pabs are a collection of immunoglobulin molecules that react against a specific antigen, each identifying a different epitope.
-
Host
Rabbit, Rabbits
-
Isotype
Immunoglobulin
-
Label
Unconjugated
-
Species
Human, Humans
-
Alternative name
production species: rabbit Antibody toHuman ATG10 polyclonal: nonconjugated
-
Alternative technique
polyclonals, rabbit-anti, antibodies
-
