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Immunogen
Synthetic peptide of Human alpha-Tubulin
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Antibody s target
Human alpha Tubulin Antibody
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Antibody s full description
Rabbit Anti-Human alpha Tubulin Antibody Polyclonal
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Primary research fields
Tags and Cell Markers, Organelle Markers or subcellular markers, Cell Signaling, Cytoskeleton, Microtubules
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Antibody s category
Polyclonal Antibodies
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Antibody s other name
Alpha-tubulin ubiquitous Antibody, Alpha tubulin ubiquitous Antibody, Tubulin alpha 1B chain Antibody, Tubulin alpha ubiquitous Antibody, Tubulin K-alpha-1 Antibody, TBA1B_HUMAN Antibody, K alpha 1 Antibody, Tubulin alpha ubiquitous chain Antibody, Tubulin alpha 1B Antibody, TUBA1B Antibody, Tubulin alpha-1B chain Antibody, Tubulin alpha-ubiquitous chain Antibody, Tubulin K alpha 1 Antibody
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Verified applications
WB, ICC/IF
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Raised in
Rabbit
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Antibody s reactivity
Human
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Antibody s recommended dilutions for use
WB (1:1000); ICC/IF (1:100); optimal dilutions for assays should be determined by the user.
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Antibody s purified from
Peptide Affinity Purified
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Recommended buffer for storage
PBS, 50% glycerol, 0.09% sodium azide
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Antibody s concentration
1 mg/ml
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Antibody s specificity
Detects ~50 kDa.
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Storage recommendations
-20°C
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Shipping recommendations
Blue Ice or 4°C
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Certificate of analysis
A 1:1000 dilution of SPC-692 was sufficient for detection of alpha-Tubulin in 15 µg of Human HeLa Cell Lysates by ECL immunoblot analysis using goat anti-rabbit IgG:HRP as the secondary antibody.
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Antibody in cell
Cytoplasm, Cytoskeleton
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Scientific context
See included datasheet or contact our support service.
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Bibliography
Refer to PubMed
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Released date
29/Aug/2016
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NCBI number
NP_006073.2
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Gene number
10376
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Protein number
P68363
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PubMed number
Refer to PubMed
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Tested applications
To be tested
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Tested reactivity
To be tested
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Antibody s datasheet
Contact our support service to receive datasheet or other technical documentation.
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Representative figure link
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Representative figure legend
Immunocytochemistry/Immunofluorescence analysis using Rabbit Anti-alpha Tubulin Polyclonal Antibody (SPC-692). Tissue: NIH 3T3 (Mouse Fibroblast cell line). Species: Mouse. Fixation: 4% Formaldehyde for 15 min at RT. Primary Antibody: Rabbit Anti-alpha Tubulin Polyclonal Antibody (SPC-692) at 1:100 for 60 min at RT. Secondary Antibody: Goat Anti-Rabbit ATTO 488 at 1:200 for 60 min at RT. Counterstain: Phalloidin Texas Red F-Actin stain; DAPI (blue) nuclear stain at 1:1000, 1:5000 for 60 min at RT, 5 min at RT. Localization: Cytoplasm, Cytoskeleton. Magnification: 60X. (A) DAPI (blue) nuclear stain (B) Phalloidin Texas Red F-Actin stain (C) alpha Tubulin Antibody (D) Composite. | Western blot analysis of Human A549 cell lysates showing detection of 50.1 kDa alpha Tubulin Antibody protein using Rabbit Anti-alpha Tubulin Antibody Polyclonal Antibody (SPC-692). Lane 1: Molecular Weight Ladder (MW). Lane 2: Human A549 cell lysates. Load: 15 µg . Block: 2% BSA and 2% Skim Milk in 1X TBST. Primary Antibody: Rabbit Anti-alpha Tubulin Antibody Polyclonal Antibody (SPC-692) at 1:1000 for 16 hours at 4°C. Secondary Antibody: Goat Anti-Rabbit IgG: HRP at 1:2000 for 60 min at RT. Color Development: ECL solution for 6 min in RT. Predicted/Observed Size: 50.1 kDa. | Western blot analysis of Human HeLa cell lysates showing detection of 50.1 kDa alpha Tubulin Antibody protein using Rabbit Anti-alpha Tubulin Antibody Polyclonal Antibody (SPC-692). Lane 1: Molecular Weight Ladder (MW). Lane 2: Human HeLa cell lysates. Load: 15 µg . Block: 5% Skim Milk in 1X TBST. Primary Antibody: Rabbit Anti-alpha Tubulin Antibody Polyclonal Antibody (SPC-692) at 1:1000 for 16 hours at 4°C. Secondary Antibody: Goat-Anti-Rabbit IgG: HRP at 1:2000 for 60 min at RT. Color Development: TMB. Predicted/Observed Size: 50.1 kDa. Rabbit Anti-alpha Tubulin Antibody used in Immunocytochemistry/Immunofluorescence (ICC/IF) on NIH 3T3 (Mouse Fibroblast cell line) (SPC-692) | Rabbit Anti-alpha Tubulin Antibody used in Western blot (WB) on A549 cell lysates (SPC-692) | Rabbit Anti-alpha Tubulin Antibody used in Western blot (WB) on HeLa cell lysates (SPC-692)
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Warning information
Non-hazardous
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Country of production
Canada
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Total weight kg
1.4
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Net weight g
0.1
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Stock availabilit
In Stock
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Description
The Rabbit Anti- alpha Tubulin Antibody Polyclonal: Streptavidin is a α- or alpha protein sometimes glycoprotein present in blood. This antibody needs to be stored at + 4°C in a fridge short term in a concentrated dilution. Freeze thaw will destroy a percentage in every cycle and should be avoided. Antibody for research use.
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Properties
If you buy Antibodies supplied by StressMark antibodies they should be stored frozen at - 24°C for long term storage and for short term at + 5°C. Human proteins, cDNA and human recombinants are used in human reactive ELISA kits and to produce anti-human mono and polyclonal antibodies. Modern humans (Homo sapiens, primarily ssp. Homo sapiens sapiens). Depending on the epitopes used human ELISA kits can be cross reactive to many other species. Mainly analyzed are human serum, plasma, urine, saliva, human cell culture supernatants and biological samples.
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Test
StressMark antibodies supplies antibodies that are for research of human proteins.
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Group
Polyclonals and antibodies
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About
Polyclonals can be used for Western blot, immunohistochemistry on frozen slices or parrafin fixed tissues. The advantage is that there are more epitopes available in a polyclonal antiserum to detect the proteins than in monoclonal sera. Rabbits are used for polyclonal antibody production by StressMark antibodies. Rabbit antibodies are very stable and can be stored for several days at room temperature. StressMark antibodies adds sodium azide and glycerol to enhance the stability of the rabbit polyclonal antibodies. Anti-human, anti mouse antibodies to highly immunogenic selected peptide sequences are" monoclonal like" since the epitope to which they are directed is less than 35 amino acids long.
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Latin name
Oryctolagus cuniculus
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French translation
anticorps
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Gene target
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Short name
Rabbit Anti- alpha Tubulin Antibody Polyclonal: Streptavidin
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Technique
anti-Human, Polyclonal, Antibody, Rabbit, anti-, anti, antibody to, antibodies, antibodies against human proteins, antibodies for, antibodies against human proteins, Polyclonal antibodies (pAbs) are mostly rabbit or goat antibodies that are secreted by different B cells, whereas monoclonal antibodies come from a single N cell lineage. Pabs are a collection of immunoglobulin molecules that react against a specific antigen, each identifying a different epitope.
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Host
Rabbit, Rabbits
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Isotype
Immunoglobulin
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Label
Streptavidin
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Species
Human, Humans
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Alternative name
production species: rabbit Antibody toHuman a Tubulin (antibody to-) polyclonal: biotinilated-binding streptavidin
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Alternative technique
polyclonals, antibodies, rabbit-anti
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