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Immunogen
Human 20S proteasome extracted from erythrocytes
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Antibody s target
Human 20S Proteasome
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Antibody s full description
Rabbit Anti-Human 20S Proteasome Polyclonal
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Primary research fields
Cancer, Apoptosis
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Antibody s category
Polyclonal Antibodies
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Antibody s other name
20S core particle Antibody, Macropain Antibody, Multicatalytic endopeptidase complex subunit Antibody, Prosome subunit Antibody, Proteasome subunit alpha type 1 Antibody, Proteasome subunit alpha type 5 Antibody, Proteasome subunit alpha type 7 Antibody, Proteasome subunit Antibody, Proteasome subunit beta type 1 Antibody, Proteasome subunit beta type 5 Antibody, Proteasome subunit beta type 6 Antibody, Proteasome subunit beta type 7 Antibody, PSMA1 Antibody, PSMA5 Antibody, PSMA7 Antibody, PSMB1 Antibody, PSMB5 Antibody, PSMB6 Antibody, PSMB7 Antibody, ZETA Antibody
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Verified applications
WB, ICC/IF, IP
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Raised in
Rabbit
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Antibody s reactivity
Human, Mouse
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Antibody s recommended dilutions for use
WB (1:5000); optimal dilutions for assays should be determined by the user.
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Antibody s purified from
Rabbit Antiserum
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Recommended buffer for storage
Rabbit Antiserum, 0.1% sodium azide
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Antibody s concentration
See included datasheet or contact our support service
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Antibody s specificity
Detects multiple 20S proteasome subunits.
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Storage recommendations
4°C
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Shipping recommendations
Blue Ice or 4°C
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Certificate of analysis
A 1:1000 dilution of SPC-215 was sufficient for detection of 20S proteasome ‘core’ subunits in 0.1 µg of human 26S proteasome by electrochemiluminescence analysis using goat anti-rabbit IgG:HRP as the secondary antibody.
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Antibody in cell
Cytoplasm
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Tissue specificity
See included datasheet or contact our support service.
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Scientific context
Proteasomes function in the degradation of unusable or damaged cellular proteins. Proteasome complexes are therefore localized in the nucleus and cytoplasm of eukaryotic cells. The most commonly found proteasome in mammalian cytosol is the 26S proteasome, which is made up of one 20S proteasome core subunit and two 19S subunits. The 20S core particle is in itself made up of two alpha subunits and two beta subunits, which represent the catalytic component. Proteins destined for degradation are first labeled by polyubiquitination before they enter the proteasome core and are broken down.
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Bibliography
1. Saeki Y., & Tanaka K. (2012) Methods Mol Biol. 832: 315-37. 2. Geng F., Wenzel S., & Tansey W. (2012) Annu Rev Biochem. 81:177-201.
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Released date
12/Jun/2014
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NCBI number
Refer to NCBI
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Gene number
Refer to GenBank
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Protein number
Refer to Swiss-Prot
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PubMed number
Refer to PubMed
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Tested applications
To be tested
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Tested reactivity
To be tested
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Antibody s datasheet
Contact our support service to receive datasheet or other technical documentation.
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Representative figure link
No Data Available
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Warning information
Non-hazardous
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Country of production
Canada
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Total weight kg
1.4
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Net weight g
0.1
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Stock availabilit
In Stock
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Description
This antibody needs to be stored at + 4°C in a fridge short term in a concentrated dilution. Freeze thaw will destroy a percentage in every cycle and should be avoided. Antibody for research use.
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Test
StressMark antibodies supplies antibodies that are for research of human proteins.
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Properties
Human proteins, cDNA and human recombinants are used in human reactive ELISA kits and to produce anti-human mono and polyclonal antibodies. Modern humans (Homo sapiens, primarily ssp. Homo sapiens sapiens). Depending on the epitopes used human ELISA kits can be cross reactive to many other species. Mainly analyzed are human serum, plasma, urine, saliva, human cell culture supernatants and biological samples.
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Conjugate
Peridinin chlorophyll (PerCP)
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Group
Polyclonals and antibodies
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About
Polyclonals can be used for Western blot, immunohistochemistry on frozen slices or parrafin fixed tissues. The advantage is that there are more epitopes available in a polyclonal antiserum to detect the proteins than in monoclonal sera. Rabbits are used for polyclonal antibody production by StressMark antibodies. Rabbit antibodies are very stable and can be stored for several days at room temperature. StressMark antibodies adds sodium azide and glycerol to enhance the stability of the rabbit polyclonal antibodies. Anti-human, anti mouse antibodies to highly immunogenic selected peptide sequences are" monoclonal like" since the epitope to which they are directed is less than 35 amino acids long.
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Latin name
Oryctolagus cuniculus
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Gene target
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Gene symbol
PSMB6, PSMB10, PSMA7
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Short name
Rabbit Anti- 20S Proteasome Polyclonal: PerCP
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Technique
anti-Human, Polyclonal, Rabbit, anti-, Percp, anti, antibody to, antibodies, antibodies against human proteins, Peridinin chlorophyll protein (PerCP) has a 677 nanometer maximum emission, red, when excited at 488 nanometers and the Rabbit Anti- 20S Proteasome Polyclonal: PerCP is detected on the FL3 detector of a FACSCalibur or FACScan. A PerCP dye is also available (PerCP-Cy5.5, also written Cy5.5-PerCP). PerCP is not suited for the high-powered lasing (>150mW) applications, such as on a MoFlo, due to it photobleaching (features/ qualities/ traits). PerCP conjugates can only be received/be gotten from the Becton Dickinson/Pharmingen group of companies or from StressMark antibodies. Polyclonal antibodies (pAbs) are mostly rabbit or goat antibodies that are secreted by different B cells, whereas monoclonal antibodies come from a single N cell lineage. Pabs are a collection of immunoglobulin molecules that react against a specific antigen, each identifying a different epitope.
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Host
Rabbit, Rabbits
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Isotype
Immunoglobulin
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Label
PerCP
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Species
Human, Humans
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Alternative name
production species: rabbit Antibody toHuman 20S protein degradation aggregate polyclonal: PerCP
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Alternative technique
polyclonals, rabbit-anti, antibodies-percp, antibodies
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