-
Immunogen
Synthetic peptide derived from the sequence of hamster HSP110; sequence identical to human and mouse
-
Antibody s target
Hamster HSP110
-
Antibody s full description
Rabbit Anti-Hamster HSP110 Polyclonal
-
Primary research fields
Cancer, Heat Shock
-
Antibody s category
Polyclonal Antibodies
-
Antibody s other name
Heat shock 105kDa alpha Antibody, heat shock 105kDa beta Antibody, HSP105 Antibody, HSP105A Antibody, HSP105B Antibody, HSP110 Antibody, HSPH1 Antibody, NY CO 25 Antibody
-
Verified applications
WB, IHC
-
Raised in
Rabbit
-
Antibody s reactivity
Human, Mouse, Rat, Bovine, Monkey, Hamster, Sheep, Yeast, Shark, Gummy Shark (Mustelus antarcticus), School Shark (Galeorhinus galeus)
-
Antibody s recommended dilutions for use
WB (1:1000), IHC (1:100); optimal dilutions for assays should be determined by the user.
-
Antibody s purified from
Protein A Purified
-
Recommended buffer for storage
PBS pH7.2, 50% glycerol, 0.09% sodium azide
-
Antibody s concentration
1 mg/ml
-
Antibody s specificity
Detects ~110kDa.
-
Storage recommendations
-20°C
-
Shipping recommendations
Blue Ice or 4°C
-
Certificate of analysis
1 µg/ml of SPC-195 was sufficient for detection of HSP110 in 10 µg of human cell line mixed lysate by colorimetric immunoblot analysis using Goat anti-rabbit IgG:HRP as the secondary antibody.
-
Antibody in cell
Cytoplasm
-
Tissue specificity
See included datasheet or contact our support service.
-
Scientific context
HSP110 belongs to a family of large stress proteins known as the HSP110/SSE Family. The proteins in this family are the most distantly known relatives of the well studied HSP70 family. They share 30-33% amino acid identity, mostly in the conserved ATP-binding domain (1). HSP110 cooperates with HSP70 in protein folding in the eukaryotic cytosol (2). In mammals, HSP110 is constitutively expressed, but exhibits particularly high levels in the brain. Both HSP70 and HSP110 are elevated after cerebral ischemia. Recent studies demonstrate that the protective effects of HSP110 deficiency in cerebral ischemia may partly be mediated by an increase in the chaperone activity of HSP70 (3). Studies also suggest that HSP110 can be used in heat shock protein-based cancer immunotherapy (4).
-
Bibliography
1. Lee-Yoon D., et al. (1995) J Biol Chem. 270: 15725-15733. 2. Dragovic Z., et al. (2006) EMBO J. 25: 2519-2528. 3. Nakamura J., et al. (2008) Stroke. 39(10): 2853-2859. 4. Subject D.R. (2001) Health Research Inc. Report number AS90893: 1-84.
-
Released date
23/Feb/2010
-
NCBI number
NP_006635.3
-
Gene number
10808
-
Protein number
Q92599
-
PubMed number
27706135|27207636|25430986
-
Tested applications
Western Blot|Western Blot|Western Blot
-
Tested reactivity
Human |Shark|Mouse
-
Antibody s datasheet
Contact our support service to receive datasheet or other technical documentation.
-
Representative figure link
-
Representative figure legend
Immunohistochemistry analysis using Rabbit Anti-HSP110 Polyclonal Antibody (SPC-195). Tissue: backskin. Species: Mouse. Fixation: Bouin's Fixative Solution. Primary Antibody: Rabbit Anti-HSP110 Polyclonal Antibody (SPC-195) at 1:100 for 1 hour at RT. Secondary Antibody: FITC Goat Anti-Rabbit (green) at 1:50 for 1 hour at RT. Localization: Positive epidermal, dermal and muscle staining. | Western blot analysis of Human Cell line lysates showing detection of HSP110 protein using Rabbit Anti-HSP110 Polyclonal Antibody (SPC-195). Load: 15 µg protein. Block: 1.5% BSA for 30 minutes at RT. Primary Antibody: Rabbit Anti-HSP110 Polyclonal Antibody (SPC-195) at 1:1000 for 2 hours at RT. Secondary Antibody: Donkey Anti-Rabbit IgG: HRP for 1 hour at RT. Rabbit Anti-HSP110 Antibody used in Immunohistochemistry (IHC) on Mouse backskin (SPC-195) | Rabbit Anti-HSP110 Antibody used in Western blot (WB) on Human Cell line lysates (SPC-195)
-
Warning information
Non-hazardous
-
Country of production
Canada
-
Total weight kg
1.4
-
Net weight g
0.1
-
Stock availabilit
In Stock
-
-
Description
This antibody needs to be stored at + 4°C in a fridge short term in a concentrated dilution. Freeze thaw will destroy a percentage in every cycle and should be avoided. Antibody for research use.
-
Properties
Armenian of Syrian hamster monoclonal IgGs are useful antibodies for double staining your IHC samples. Using mabs or monoclonal antibodies has a lot of advantages for Flow cytometry.
-
Group
Polyclonals and antibodies
-
About
Polyclonals can be used for Western blot, immunohistochemistry on frozen slices or parrafin fixed tissues. The advantage is that there are more epitopes available in a polyclonal antiserum to detect the proteins than in monoclonal sera. Rabbits are used for polyclonal antibody production by StressMark antibodies. Rabbit antibodies are very stable and can be stored for several days at room temperature. StressMark antibodies adds sodium azide and glycerol to enhance the stability of the rabbit polyclonal antibodies. Anti-human, anti mouse antibodies to highly immunogenic selected peptide sequences are" monoclonal like" since the epitope to which they are directed is less than 35 amino acids long.
-
Latin name
Oryctolagus cuniculus
