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Products_type
Secondary Antibody
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Products_short_name
[IgG (H&L), F(ab)'2 fragment]
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Products_name_syn
[Goat anti-mouse IgG (H&L), F(ab)'2 fragment]
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Reactivity
Mouse. Cross Reactivity: Based on IEP, no reactivity is observed to: non-immunoglobulin mouse serum proteins bovine, goat, human, rabbit or rat IgG Based on ELISA, this antibody has
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Specificity
Based on IEP, this antibody reacts with: heavy (gamma) chains on mouse IgG, light chains on all mouse immunoglobulins
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Purity
> 90% based on SDS-PAGE Small amounts of intact IgG may be present. Affinity purified using solid phase Mouse IgG.
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Form
Lyophilized
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Concentration
1.0 mg/ml (E 1% at 280 nm = 13.0)
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Storage_stability
Store at 2-8 degree C. Shelf Life: 1 year from date of receipt. Prepare working dilution prior to use and then discard.
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Tested_application
Flow Cytometry (FC/FACS), Immunofluorescence (IF), ELISA (EIA), Immunomicroscopy (IM)
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Description
This antibody needs to be stored at + 4°C in a fridge short term in a concentrated dilution. Freeze thaw will destroy a percentage in every cycle and should be avoided. Antibody for research use.
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Latin name
Capra aegagrus hircus, Mus musculus, Oryctolagus cuniculus, Rattus norvegicus
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Properties
Human proteins, cDNA and human recombinants are used in human reactive ELISA kits and to produce anti-human mono and polyclonal antibodies. Modern humans (Homo sapiens, primarily ssp. Homo sapiens sapiens). Depending on the epitopes used human ELISA kits can be cross reactive to many other species. Mainly analyzed are human serum, plasma, urine, saliva, human cell culture supernatants and biological samples.
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About
Immunoglobulin gamma, IgG, mouse monoclonal H&L chain clones or rabbit, goat polyclonal antibodies have 4 parts. There are 2 heavy chains, 2 light chains. The IgG antibody has 2 antigen binding sites. They represent 70% or more of serum antibodies. This antibody can be antigen purified or protein A or G purified. For storage sodium azide is added or you can call us to request azide free antibody preparations. These will need colder storage temperatures. Rabbits are used for polyclonal antibody production by MyBioSource. Rabbit antibodies are very stable and can be stored for several days at room temperature. MyBioSource adds sodium azide and glycerol to enhance the stability of the rabbit polyclonal antibodies. Anti-human, anti mouse antibodies to highly immunogenic selected peptide sequences are" monoclonal like" since the epitope to which they are directed is less than 35 amino acids long. Rats are used to make rat monoclonal anti mouse antibodies. There are less rat- than mouse clones however. Rats genes from rodents of the genus Rattus norvegicus are often studied in vivo as a model of human genes in Sprague-Dawley or Wistar rats.
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Test
Mouse or mice from the Mus musculus species are used for production of mouse monoclonal antibodies or mabs and as research model for humans in your lab. Mouse are mature after 40 days for females and 55 days for males. The female mice are pregnant only 20 days and can give birth to 10 litters of 6-8 mice a year. Transgenic, knock-out, congenic and inbread strains are known for C57BL/6, A/J, BALB/c, SCID while the CD-1 is outbred as strain.
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Gene target
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Short name
Goat anti-Mouse IgG (H L) - F(ab)'2 , TRITC Conjugate, min x w/ , goat, , rabbit, IgG (highly absorbed against IgG)
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Technique
Rabbit, anti-, Mouse, anti, Goat, IgG, antibody to, antibodies, goats, IgGs, mouses
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Host
Goat, The goat polyclonal antibodies are directed against human genes or rabbit or mouse IgGs (H +L) chain primary antibodies and conjugated for detection. It are very good secondary antibodies that are affinity purified and have a good affinity themselves too. Often KLH is coupled to the antigen so anti KLH can be present in the vial. Rabbits
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Isotype
IgG, Antibody fragments and scFv fragments, single chain variable fragments can be E. coli expressed. Fragment peptides for polyclonals give monoclonal like antibodies as the epitope is very small. IgG Fc and(ab)2 fragments are small and stable subunits of the antibody obtained by enzymatic digestion. Complement fragments, bp DNA fragments are common. Heavy & Light chains, IgG
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Label
tritc
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Species
Rat, Bos Taurus genes are available form the Bovine Genome database. Goats, Humans, Mouses, Rats
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Alternative name
caprine Antibody toMouse Immunoglobulin G (heavy + light chain) - F(Antibody)'2 fragment, TRITC Conjugate, min x w/bovine, caprine, H. sapiens, production species: rabbit, and rat Immunoglobulin G (very absorbed against rat Immunoglobulin G)
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Alternative technique
rabbit-anti, murine, antibodies, caprine, immunoglobulins
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