Flag-Tag polyclonal antibody

• Catalog number
  E0T503-2
• Price
  Please ask
• Size
  100ug

• Conjugation
  Unconjugated
• Note
  For research use only.
• Properties
  If you buy Antibodies supplied by EnoGene they should be stored frozen at - 24°C for long term storage and for short term at + 5°C. An anti-flag tag (FLAG fusion protein) is use to detect a FLAG-tag, or FLAG octapeptide, or FLAG epitope that is a polypeptide protein tag that can be added to a protein using recombinant DNA. This FLAG-tags have the sequence DYKDDDDK motiv.  These tags are very useful to do protein purification by affinity chromatography. Also separation of recombinant, overexpressed proteins from cell lysates is done by FLAG go HIS tags. FLAGS are also used in the isolation of protein complexes with multiple subunits, because its mild purification procedure tends not to disrupt such complexes. It has been used to enrich proteins of height purity and quality to see the 3D crystal structure with x-ray. Suitable for in vivo use in cells. For electrophorese protein detection rabbit polyclonals anti Flag conjugation are the most suited antibodies.
• Additional conjugation
  Flag
• Group
  Polyclonals and antibodies
• About
  Polyclonals can be used for Western blot, immunohistochemistry on frozen slices or parrafin fixed tissues. The advantage is that there are more epitopes available in a polyclonal antiserum to detect the proteins than in monoclonal sera.
• French translation
  anticorps

• Gene target
  Tag  
• Gene symbol
  TRL-TAG2-1, TRL-TAG3-1, TRL-TAG4-1
• Short name
  -Tag polyclonal antibody
• Technique
  Polyclonal, Antibody, antibodies against human proteins, antibodies for, Polyclonal antibodies (pAbs) are mostly rabbit or goat antibodies that are secreted by different B cells, whereas monoclonal antibodies come from a single N cell lineage. Pabs are a collection of immunoglobulin molecules that react against a specific antigen, each identifying a different epitope. Tags
• Label
  Flag
• Alternative name
  Flag-detection labelled polyclonal (antibody to-)
• Alternative technique
  polyclonals, antibodies

Gene info

• Identity
  HGNC:34713
• Gene
  TRL-TAG2-1
• Long gene name
  tRNA-Leu (anticodon TAG) 2-1
• Synonyms gene
  • TRNAL15
• Synonyms gene name
  • transfer RNA leucine 15 (anticodon UAG)
  • transfer RNA-Leu (TAG) 2-1
• Synonyms
  • tRNA-Leu-TAG-2-1
• GenBank acession
  • HG983905
• Locus
  14q11.2
• Discovery year
  2008-08-29
• Entrez gene record
  100189151
• Classification
  • Cytoplasmic transfer RNAs

Gene info

• Identity
  HGNC:34591
• Gene
  TRL-TAG3-1
• Long gene name
  tRNA-Leu (anticodon TAG) 3-1
• Synonyms gene
  • TRNAL7
• Synonyms gene name
  • transfer RNA leucine 7 (anticodon UAG)
  • transfer RNA-Leu (TAG) 3-1
• Synonyms
  • tRNA-Leu-TAG-3-1
• GenBank acession
  • HG983906
• Locus
  16p12.2
• Discovery year
  2008-08-29
• Entrez gene record
  100189032
• Classification
  • Cytoplasmic transfer RNAs

Gene info

• Identity
  HGNC:35011
• Gene
  TRL-TAG4-1
• Long gene name
  tRNA-Leu (anticodon TAG) 4-1
• Synonyms gene
  • TRNAL40P
• Synonyms gene name
  • transfer RNA leucine 40 (anticodon UAG) pseudogene
  • transfer RNA-Leu (TAG) 4-1
• Synonyms
  • tRNA-Leu-TAG-4-1
• GenBank acession
  • HG983907
• Locus
  14q11.2
• Discovery year
  2008-08-29
• Entrez gene record
  100189441
• RefSeq identity
  • NG_008497
• Classification
  • Cytoplasmic transfer RNA pseudogenes

MeSH Data

• Name
  Blotting, Western
• Concept
  Scope note: Identification of proteins or peptides that have been electrophoretically separated by blot transferring from the electrophoresis gel to strips of nitrocellulose paper, followed by labeling with antibody probes.
• Tree numbers
  • E05.196.401.143
  • E05.301.300.096
  • E05.478.566.320.200
  • E05.601.262
  • E05.601.470.320.200
• Qualifiers
  ethics, trends, veterinary, history, classification, economics, instrumentation, methods, standards, statistics & numerical data

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