Rabbit anti Flag-Tag Polyclonal Antibody

  • Catalog number
    E16TPU007-100-FITC
  • Price
    Please ask
  • Size
    100Tests
  • Conjugation
    FITC
  • Note
    For research use only.
  • Description
    This antibody needs to be stored at + 4°C in a fridge short term in a concentrated dilution. Freeze thaw will destroy a percentage in every cycle and should be avoided.
  • Properties
    If you buy Antibodies supplied by EnoGene they should be stored frozen at - 24°C for long term storage and for short term at + 5°C. An anti-flag tag (FLAG fusion protein) is use to detect a FLAG-tag, or FLAG octapeptide, or FLAG epitope that is a polypeptide protein tag that can be added to a protein using recombinant DNA. This FLAG-tags have the sequence DYKDDDDK motiv.  These tags are very useful to do protein purification by affinity chromatography. Also separation of recombinant, overexpressed proteins from cell lysates is done by FLAG go HIS tags. FLAGS are also used in the isolation of protein complexes with multiple subunits, because its mild purification procedure tends not to disrupt such complexes. It has been used to enrich proteins of height purity and quality to see the 3D crystal structure with x-ray. Suitable for in vivo use in cells. For electrophorese protein detection rabbit polyclonals anti Flag conjugation are the most suited antibodies.
  • Additional conjugation
    Flag
  • Group
    Polyclonals and antibodies
  • About
    Polyclonals can be used for Western blot, immunohistochemistry on frozen slices or parrafin fixed tissues. The advantage is that there are more epitopes available in a polyclonal antiserum to detect the proteins than in monoclonal sera. Rabbits are used for polyclonal antibody production by EnoGene. Rabbit antibodies are very stable and can be stored for several days at room temperature. EnoGene adds sodium azide and glycerol to enhance the stability of the rabbit polyclonal antibodies. Anti-human, anti mouse antibodies to highly immunogenic selected peptide sequences are" monoclonal like" since the epitope to which they are directed is less than 35 amino acids long.
  • Latin name
    Oryctolagus cuniculus
  • French translation
    anticorps
  • Gene target
    Tag  
  • Gene symbol
    TRL-TAG2-1, TRL-TAG3-1, TRL-TAG4-1
  • Short name
    Rabbit anti -Tag Polyclonal Antibody
  • Technique
    Polyclonal, Antibody, Rabbit, anti, antibody to, antibodies against human proteins, antibodies for, Polyclonal antibodies (pAbs) are mostly rabbit or goat antibodies that are secreted by different B cells, whereas monoclonal antibodies come from a single N cell lineage. Pabs are a collection of immunoglobulin molecules that react against a specific antigen, each identifying a different epitope. Tags
  • Host
    Rabbit, Rabbits
  • Label
    Flag
  • Alternative name
    production species: rabbit antibody to Flag-detection labelled polyclonal (antibody to-)
  • Alternative technique
    polyclonals, antibodies, rabbit-anti
Gene info
  • Identity
  • Gene
  • Long gene name
    tRNA-Leu (anticodon TAG) 2-1
  • Synonyms gene
  • Synonyms gene name
    • transfer RNA leucine 15 (anticodon UAG)
    • transfer RNA-Leu (TAG) 2-1
  • Synonyms
  • GenBank acession
  • Locus
  • Discovery year
    2008-08-29
  • Entrez gene record
  • Classification
    • Cytoplasmic transfer RNAs
Gene info
  • Identity
  • Gene
  • Long gene name
    tRNA-Leu (anticodon TAG) 3-1
  • Synonyms gene
  • Synonyms gene name
    • transfer RNA leucine 7 (anticodon UAG)
    • transfer RNA-Leu (TAG) 3-1
  • Synonyms
  • GenBank acession
  • Locus
  • Discovery year
    2008-08-29
  • Entrez gene record
  • Classification
    • Cytoplasmic transfer RNAs
Gene info
  • Identity
  • Gene
  • Long gene name
    tRNA-Leu (anticodon TAG) 4-1
  • Synonyms gene
  • Synonyms gene name
    • transfer RNA leucine 40 (anticodon UAG) pseudogene
    • transfer RNA-Leu (TAG) 4-1
  • Synonyms
  • GenBank acession
  • Locus
  • Discovery year
    2008-08-29
  • Entrez gene record
  • RefSeq identity
  • Classification
    • Cytoplasmic transfer RNA pseudogenes
MeSH Data
  • Name
  • Concept
    Scope note: Identification of proteins or peptides that have been electrophoretically separated by blot transferring from the electrophoresis gel to strips of nitrocellulose paper, followed by labeling with antibody probes.
  • Tree numbers
    • E05.196.401.143
    • E05.301.300.096
    • E05.478.566.320.200
    • E05.601.262
    • E05.601.470.320.200
  • Qualifiers
    ethics, trends, veterinary, history, classification, economics, instrumentation, methods, standards, statistics & numerical data
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