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Immunogen
Dog calnexin C-terminal synthetic peptide conjugated to KLH. Identical to human, mouse and rat calnexin sequences over these residues.
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Antibody s target
Dog Calnexin
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Antibody s full description
Rabbit Anti-Dog Calnexin-CT Polyclonal
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Primary research fields
Cell Signaling, Organelle Markers
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Antibody s category
Polyclonal Antibodies
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Antibody s other name
Calnexin antibody, CALX_HUMAN antibody, CANX antibody, CNX antibody, FLJ26570 antibody, Histocompatibility complex class I antigen binding protein p88 antibody, IP90 antibody, Major histocompatibility complex class I antigen-binding protein p88 antibody, P90 antibody
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Verified applications
WB, ICC/IF, IHC
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Raised in
Rabbit
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Antibody s reactivity
Human, Mouse, Rat, Bovine, Monkey, Chicken, Dog, Guinea Pig (Cavia porcellus), Hamster, Pig, Avian, Quail, Rabbit, Sheep, Fruit Fly (Drosophila melanogaster), African clawed frog (Xenopus laevis)
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Antibody s recommended dilutions for use
WB (1:1000), IHC (1:100), ICC/IF (1:100); optimal dilutions for assays should be determined by the user.
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Antibody s purified from
Peptide Affinity Purified
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Recommended buffer for storage
PBS pH7.4, 50% glycerol, 0.09% sodium azide
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Antibody s concentration
1 mg/ml
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Antibody s specificity
Detects the C-terminal domain of Calnexin ~90kDa. Weak detection in Chicken, Drosophila, and Xenopus tissues
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Storage recommendations
-20°C
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Shipping recommendations
Blue Ice or 4°C
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Certificate of analysis
A 1:1000 dilution of SPC-108 was sufficient for detection of Calnexin in 10 µg of HeLa cell lysate by ECL immunoblot analysis.
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Antibody in cell
Endoplasmic Reticulum, Endoplasmic Reticulum Lumen, Melanosome, Cytoplasm
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Tissue specificity
See included datasheet or contact our support service.
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Scientific context
Calnexin, an abundant ~90kDa integral protein of the endoplasmic reticulum, is also referred to as IP90, p88 and p90 (1). It consists of a large 50kDa N-terminal calcium-binding luminal domain, a single transmembrane helix and a short acidic cytoplasmic tail (2, 3). Unlike its ER counterparts which have a KDEL sequence on their C-terminus to ensure ER retention (4), calnexin has positively charged cytosolic residues that do the same thing (3). Most ER proteins act as molecular chaperones and participate in the proper folding of polypeptides and their assembly into multi-subunit proteins. Calnexin together with calreticulin, plays a key role in glycoprotein folding and its control within the ER, by interacting with folding intermediates via their mono-glycosylated glycans (5, 6). Calnexin has also been shown to associate with the major histocompatibility complex class I heavy chains, partial complexes of the T cell receptor and B cell membrane immunoglobulin (7).
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Bibliography
1. Rajagopalan S., Xu Y., and Brenner M.B. (1994) Science 263(5145): 387-90. 2. Tjoelker L.W., et al. (1994) Biochemistry 33: 3229. 3. Schrag J. et al. (2001) Molecular Cell 8(3): 633-644. 4. Janiszewski M. (2005) J. Biol Chem. 280(49): 40813-40819. 5. Elagoz A., Callejo M., Armstrong J., and Rokeach L. A. (1999) J. Cell Sci. 112: 4449-4460. 6. Otteken A. and Moss B. (1996) J Bio Chem. 271(1): 97-103. 7. Galvin K. et al. (1992) Proc Natl Acad Sci USA. 89(18): 8452-6.
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Released date
1/Nov/2008
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NCBI number
NP_001003232.1
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Gene number
403908
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Protein number
P24643
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PubMed number
27635674|26993334|26900700|26833332|26666627|26153658|24594635|25038744|25122799
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Tested applications
Western Blot|Immunocytochemistry/Immunofluorescence|Immunocytochemistry/Immunofluorescence|Immunocytochemistry/Immunofluorescence|Immunohistochemistry|Immunocytochemistry/Immunofluorescence|Immunocytochemistry/Immunofluorescence|Immunocytochemistry/Immunofluorescence|Immunocytochemistry/Immunofluorescence
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Tested reactivity
Human|Mouse|Dog|Human|Rat|Human|Human|Mouse|Human
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Antibody s datasheet
Contact our support service to receive datasheet or other technical documentation.
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Representative figure link
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Representative figure legend
Immunocytochemistry/Immunofluorescence analysis using Rabbit Anti-Calnexin Polyclonal Antibody (SPC-108). Tissue: HeLa cells. Species: Human. Primary Antibody: Rabbit Anti-Calnexin Polyclonal Antibody (SPC-108) at 1:100. Secondary Antibody: FITC Goat Anti-Rabbit (green). | Western blot analysis of Human HeLa cell lysates showing detection of Calnexin protein using Rabbit Anti-Calnexin Polyclonal Antibody (SPC-108). Primary Antibody: Rabbit Anti-Calnexin Polyclonal Antibody (SPC-108) at 1:1000. | Immunohistochemistry analysis using Rabbit Anti-Calnexin Polyclonal Antibody (SPC-108). Tissue: backskin. Species: Mouse. Fixation: Bouin's Fixative Solution. Primary Antibody: Rabbit Anti-Calnexin Polyclonal Antibody (SPC-108) at 1:100 for 1 hour at RT. Secondary Antibody: FITC Goat Anti-Rabbit (green) at 1:50 for 1 hour at RT. | Western blot analysis of Rat Tissue lysates showing detection of Calnexin protein using Rabbit Anti-Calnexin Polyclonal Antibody (SPC-108). Load: 15 µg protein. Block: 1.5% BSA. Primary Antibody: Rabbit Anti-Calnexin Polyclonal Antibody (SPC-108) at 1:1000 for 2 hours at RT. Secondary Antibody: Donkey Anti-Rabbit IgG: HRP for 1 hour at RT. Rabbit Anti-Calnexin Antibody used in Immunocytochemistry/Immunofluorescence (ICC/IF) on Human HeLa cells (SPC-108) | Rabbit Anti-Calnexin Antibody used in Western blot (WB) on Human HeLa cell lysates (SPC-108) | Rabbit Anti-Calnexin Antibody used in Immunohistochemistry (IHC) on Mouse backskin (SPC-108) | Rabbit Anti-Calnexin Antibody used in Western blot (WB) on Rat Tissue lysates (SPC-108)
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Warning information
Non-hazardous
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Country of production
Canada
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Total weight kg
1.4
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Net weight g
0.2
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Stock availabilit
In Stock
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Description
This antibody needs to be stored at + 4°C in a fridge short term in a concentrated dilution. Freeze thaw will destroy a percentage in every cycle and should be avoided. Antibody for research use.
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Properties
Canine or Canis Lupus is mostly Beagle used for drug research. StressMark antibodies produces ELISA test kits and polyclonal antibodies.
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Group
Polyclonals and antibodies
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About
Polyclonals can be used for Western blot, immunohistochemistry on frozen slices or parrafin fixed tissues. The advantage is that there are more epitopes available in a polyclonal antiserum to detect the proteins than in monoclonal sera. Rabbits are used for polyclonal antibody production by StressMark antibodies. Rabbit antibodies are very stable and can be stored for several days at room temperature. StressMark antibodies adds sodium azide and glycerol to enhance the stability of the rabbit polyclonal antibodies. Anti-human, anti mouse antibodies to highly immunogenic selected peptide sequences are" monoclonal like" since the epitope to which they are directed is less than 35 amino acids long.
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Latin name
Oryctolagus cuniculus
