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Category
Primary Antibodies
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Long description
Apoptosis is characterized by several morphological nuclear changes including chromatin condensation and nuclear fragmentation. These changes are triggered by the activation of members of caspase family, caspase activated DNase, and several novel proteins including AIF and Acinus (1). DNase II causes both chromatin condensation and DNA fragmentation (1). The genes encoding human (2-4), porcine (4), and murine (5) DNase II have been cloned. The DNase II gene encodes a 40 kDa proenzyme. The mature enzyme consists of two non-identical subunits, the 32 kDa (a) and 12 kDa (b) chains, generated by proteolytic processing. Overexpression of DNase II induces chromatin condensation (3). DNase II is ubiquitously expressed in human tissues.
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Antibody come from
A synthetic peptide corresponding to amino acids 347 to 360 of human DNase II precursor (2-4).
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Other description
Provided in phosphate buffered saline solution containing 0.02% sodium azide as a preservative. Antigen Immunoaffiinity Purification
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Clone
Polyclonal
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Antigen antibody binding interaction
Rabbit anti DNase II alpha (CT) Antibody
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Antibody is raised in
Rabbit
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Antibody s reacts with
Human
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Antibody s reacts with these species
This antibody doesn't cross react with other species
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Antibody s specificity
No Data Available
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Application
Western Blot
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Antibody s suited for
Detects DNase II expression by Western blot at 0.5 to 1 µg/ml. Detects an approximately 32 kDa ( chain) band in human spleen lysates. It doesnÕt recognize the 12 kDa chain. Optimal concentration should be evaluated by serial dilutions.
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Storage
-20ºC
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Relevant references
1. Condensed matter in cell death. Zamzami, N., et al. Nature 1999, 401, 127-8_x000B__x000B_2. Molecular cloning of the cDNA encoding human deoxyribonuclease II. Yasuda, T., et al., J. _x000B_Biol. Chem. 1998, 273, 2610-6_x000B__x000B_3. The cloning and expression of human deoxyribonuclease II. A possible role in apoptosis. Krieser, R.J. and Eastman, A. J. Biol. Chem. 1998, 273, 30909-14_x000B__x000B_4. Cloning of cDNAs encoding porcine and human DNase II. Shiokawa, D., et al., Biochem. Biophys. Res. Commun. 1998, 247, 864-9_x000B__x000B_5. Molecular cloning and characterization of human and murine DNase II. Baker, K.P., et al., Gene 1998, 215, 281-9
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Protein number
see ncbi
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Warnings
This product is intended FOR RESEARCH USE ONLY, and FOR TESTS IN VITRO, not for use in diagnostic or therapeutic procedures involving humans or animals. This datasheet is as accurate as reasonably achievable, but Nordic-MUbio accepts no liability for any inaccuracies or omissions in this information.
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Description
The Rabbit anti DNase II alpha (CT) is a α- or alpha protein sometimes glycoprotein present in blood. This antibody needs to be stored at + 4°C in a fridge short term in a concentrated dilution. Freeze thaw will destroy a percentage in every cycle and should be avoided.
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About
Rabbits are used for polyclonal antibody production by nordc. Rabbit antibodies are very stable and can be stored for several days at room temperature. nordc adds sodium azide and glycerol to enhance the stability of the rabbit polyclonal antibodies. Anti-human, anti mouse antibodies to highly immunogenic selected peptide sequences are" monoclonal like" since the epitope to which they are directed is less than 35 amino acids long.
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Latin name
Oryctolagus cuniculus
