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Category
Primary Antibodies
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Long description
Members in the Bcl-2 family are critical regulators of apoptosis by either inhibiting or promoting cell death. Bcl-2 homology 3 (BH3) domain is a potent death domain. BH3 domain containing pro-apoptotic proteins, including Bad, Bax, Bid, Bik, Hrk, Nip3, and Bim, form a growing subclass of the Bcl-2 family. A novel BH3 domain containing protein was recently identified and designated Bnip3L, Bnip3 , and Nix (for Nip3-like protein X) (1-3). Bnip3L/Bnip3 /Nix is a homolog of the E1B 19K/Bcl-2 binding and pro-apoptotic protein Bnip3. Overexpression of Bnip3L induces apoptosis (2,3). Bnip3L interacts with and overcomes suppresses by Bcl-2 and Bcl-xL. Bnip3L is localized in mitochondria. The messenger RNA of Bnip3L is ubiquitously expressed in human tissues (1,2). Bnip3L and Bnip3 form a new subfamily of the pro-apoptotic mitochondrial proteins.
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Antibody come from
Synthetic peptide corresponding to amino acids 77 to 92 of the human Bnip3L protein. Immunogen sequence is identical in the mouse protein.
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Other description
Provided in phosphate buffered saline solution containing 0.02% sodium azide as a preservative. Antigen Immunoaffiinity Purification
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Clone
Polyclonal
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Antigen antibody binding interaction
Rabbit anti Bnip3L (IN) (nix) (bcl 2 family) Antibody
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Antibody is raised in
Rabbit
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Antibody s reacts with
Human
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Antibody s reacts with these species
This antibody doesn't cross react with other species
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Antibody s specificity
No Data Available
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Application
Western Blot
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Antibody s suited for
Detects Bnip3L by Western blot at 0.5 to 1 µg/ml. Detects a 40 kDa band in K562 cell lysate which represents the monomer of Bnip3L. Optimal concentration should be evaluated by serial dilutions.
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Storage
-20ºC
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Relevant references
1. Matsushima, M., et al., Isolation, mapping, and functional analysis of a novel human cDNA (BNIP3L) encoding a protein homologous to human NIP3. Genes Chromosomes Cancer 1998, 21, 230-235_x000B__x000B_2. Yasuda, M., et al., BNIP3alpha: a human homolog of mitochondrial proapoptotic protein BNIP3. Cancer Res. 1999, 59, 533-537_x000B__x000B_3. Chen, G., et al., Nix and Nip3 form a subfamily of pro-apoptotic mitochondrial proteins. J. Biol. Chem. 1999, 274, 7-10_x000B__x000B_4. Imazu, T., et al., Bcl-2/E1B 19 kDa-interacting protein 3-like protein (Bnip3L) interacts with bcl-2/Bcl-xL and induces apoptosis by altering mitochondrial membrane permeability. Oncogene. 1999, 18, 4523-4529_x000B__x000B_Product Specific References: _x000B__x000B_1. Aerbajinai, W., et al. 'The proapoptotic factor Nix is coexpressed with Bcl-xL during terminal erythroid differentiation.' Blood, 2003, 102, 712-717._x000B__x000B_2. Zhang, J., et al. 'Mitochondrial clearance is regulated by Atg7-dependent and -independent mechanisms during reticulocyte maturation.' Blood, 2009, 114, 157-164.
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Protein number
see ncbi
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Warnings
This product is intended FOR RESEARCH USE ONLY, and FOR TESTS IN VITRO, not for use in diagnostic or therapeutic procedures involving humans or animals. This datasheet is as accurate as reasonably achievable, but Nordic-MUbio accepts no liability for any inaccuracies or omissions in this information.
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Description
This antibody needs to be stored at + 4°C in a fridge short term in a concentrated dilution. Freeze thaw will destroy a percentage in every cycle and should be avoided.
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About
Rabbits are used for polyclonal antibody production by nordc. Rabbit antibodies are very stable and can be stored for several days at room temperature. nordc adds sodium azide and glycerol to enhance the stability of the rabbit polyclonal antibodies. Anti-human, anti mouse antibodies to highly immunogenic selected peptide sequences are" monoclonal like" since the epitope to which they are directed is less than 35 amino acids long.
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Latin name
Oryctolagus cuniculus
