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Category
Primary Antibodies
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Long description
Chromatin condensation and nuclear fragmentation (CCNF) is the hallmark of apoptosis. CCNF is triggered by the activation of members of caspase family, caspase activated DNase (CAD/DFF40), and several novel proteins including AIF and CIDE (1). A new inducer of chromatin condensation was recently identified and designated Acinus (for apoptotic chromatin condensation inducer in the nucleus). Acinus is cleaved by caspase-3 and an additional unknown protease generating a small active peptide p17, which causes chromatin condensation in vitro when it is added to purified nuclei. Acinus also induces apoptotic chromatin condensation in cells. Acinus is ubiquitously expressed. Three different spliced forms of Acinus have been identified in human and mouse and designated AcinusL, AcinusS and AcinusSÕ (2).
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Antibody come from
Synthetic peptide (DDPVRTAQVPSPPRGK) corresponding to amino acids 994 to 1009 of human AcinusL, 267 to 282 of human AcinusSÕ, or 236 to 251 of human AcinusS, which are identical to those of mouse Acinus (2). The selected antigenic sequence is located near the N-terminus of active peptide p17.
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Other description
Provided in phosphate buffered saline solution containing 0.02% sodium azide as a preservative. Antigen Immunoaffiinity Purification
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Clone
Polyclonal
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Antigen antibody binding interaction
Rabbit anti Acinus (NP) Antibody
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Antibody is raised in
Rabbit
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Antibody s reacts with
Human
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Antibody s reacts with these species
This antibody doesn't cross react with other species
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Antibody s specificity
No Data Available
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Application
Western Blot
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Antibody s suited for
Detects Acinus by Western blot at 0.5 to 1.0 µg/ml. Detects a 220 kDa band in K562 cell lysate. Optimal concentration should be evaluated by serial dilutions.
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Storage
-20ºC
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Relevant references
1. Condensed matter in cell death. Zamzami, N and Kroemer, G. , Nature, 1999 , 401, 127-8_x000B__x000B_2. Acinus is a caspase-3-activated protein required for apoptotic chromatin condensation. Sahara S, et al., Nature, 1999, 401, 168-73
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Protein number
see ncbi
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Warnings
This product is intended FOR RESEARCH USE ONLY, and FOR TESTS IN VITRO, not for use in diagnostic or therapeutic procedures involving humans or animals. This datasheet is as accurate as reasonably achievable, but Nordic-MUbio accepts no liability for any inaccuracies or omissions in this information.
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Description
This antibody needs to be stored at + 4°C in a fridge short term in a concentrated dilution. Freeze thaw will destroy a percentage in every cycle and should be avoided.
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About
Rabbits are used for polyclonal antibody production by nordc. Rabbit antibodies are very stable and can be stored for several days at room temperature. nordc adds sodium azide and glycerol to enhance the stability of the rabbit polyclonal antibodies. Anti-human, anti mouse antibodies to highly immunogenic selected peptide sequences are" monoclonal like" since the epitope to which they are directed is less than 35 amino acids long.
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Latin name
Oryctolagus cuniculus