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Immunogen
Acetylated KLH Conjugated
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Antibody s target
Acetylated Lysine
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Antibody s full description
Rabbit Anti-Acetylated Lysine Polyclonal
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Primary research fields
Cell Signaling, Post-translational Modifications, Acetylation
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Antibody s category
Polyclonal Antibodies
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Antibody s other name
lysine Antibody, acetyl lysine Antibody
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Verified applications
WB, ICC/IF, IP, ELISA
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Raised in
Rabbit
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Antibody s reactivity
Species Independent
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Antibody s recommended dilutions for use
WB (1:250), ICC/IF (1:100); optimal dilutions for assays should be determined by the user.
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Antibody s purified from
Protein A Purified
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Recommended buffer for storage
PBS, 50% glycerol, 0.09% sodium azide
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Antibody s concentration
1 mg/ml
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Antibody s specificity
Detects proteins containing acetylated lysine residues. No reaction to non-acetylated proteins.
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Storage recommendations
-20°C
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Shipping recommendations
Blue Ice or 4°C
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Certificate of analysis
A 1/250 dilution of SPC-155 was sufficient to detect the acetylated histone from TSA treated mouse spleen cell in western blot analysis.
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Antibody in cell
Contact our support service.
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Tissue specificity
See included datasheet or contact our support service.
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Scientific context
Post-translational modifications of proteins play critical roles in the regulation and function of many known biological processes. Proteins can be post-translationally modified in many different ways, and a common post-transcriptional modification of Lysine involves acetylation (1). The conserved amino-terminal domains of the four core histones (H2A, H2B, H3 and H4) contain lysines that are acetylated by histone acetyltransferases (HATs) and deacetylated by histone deacetylases (HDACs) (2). Protein posttranslational reversible lysine Nε-acetylation and deacetylation have been recognized as an emerging intracellular signaling mechanism that plays critical roles in regulating gene transcription, cell-cycle progression, apoptosis, DNA repair, and cytoskeletal organization (3). The regulation of protein acetylation status is impaired in the pathologies of cancer and polyglutamine diseases (4), and HDACs have become promising targets for anti-cancer drugs currently in development (5).
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Bibliography
1. Yang X.J. (2005) Oncogene. 24:1653-1662. 2. Hassig C.A. and Schreiber S.L. (1997) Curr. Opin. Chem. Biol. 1(3): 300-308. 3. Yang X.J. (2004) Bioessays 26:1076-1087. 4. Hughes R.E. (2002) Curr. Biol. 12: R141-R143. 5. Vigushin D.M. and Coombes R.C. (2004) Curr. Cancer Drug Targets 4: 205-218. 6. Chan H.M. et al. (2001) Nat. Cell Biol. 3: 667-674. 7. Martinez-Balbas M.A. et al. (2000) EMBO J. 19: 662-671.
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Released date
1/Jun/2007
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NCBI number
Refer to NCBI
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Gene number
Refer to GenBank
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Protein number
Refer to Swiss-Prot
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PubMed number
21278340
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Tested applications
Western Blot
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Tested reactivity
Human
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Antibody s datasheet
Contact our support service to receive datasheet or other technical documentation.
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Representative figure link
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Representative figure legend
Immunocytochemistry/Immunofluorescence analysis using Rabbit Anti-Acetylated Lysine Polyclonal Antibody (SPC-155). Tissue: Heat Shocked HeLa Cells. Species: Human. Fixation: 2% Formaldehyde for 20 min at RT. Primary Antibody: Rabbit Anti-Acetylated Lysine Polyclonal Antibody (SPC-155) at 1:100 for 12 hours at 4°C. Secondary Antibody: R-PE Goat Anti-Rabbit (yellow) at 1:200 for 2 hours at RT. Counterstain: DAPI (blue) nuclear stain at 1:40000 for 2 hours at RT. Localization: Nucleus. Cytoplasm. Magnification: 100x. (A) DAPI (blue) nuclear stain. (B) Anti-Acetylated Lysine Antibody. (C) Composite. Heat Shocked at 42°C for 1h. | Western blot analysis of Bovine serum albumin showing detection of Acetylated Lysine protein using Rabbit Anti-Acetylated Lysine Polyclonal Antibody (SPC-155). Primary Antibody: Rabbit Anti-Acetylated Lysine Polyclonal Antibody (SPC-155) at 1:1000. Acetylated lysine in BSA (Left) and Acetylated BSA (Right). | Immunocytochemistry/Immunofluorescence analysis using Rabbit Anti-Acetylated Lysine Polyclonal Antibody (SPC-155). Tissue: Heat Shocked HeLa Cells. Species: Human. Fixation: 2% Formaldehyde for 20 min at RT. Primary Antibody: Rabbit Anti-Acetylated Lysine Polyclonal Antibody (SPC-155) at 1:100 for 12 hours at 4°C. Secondary Antibody: FITC Goat Anti-Rabbit (green) at 1:200 for 2 hours at RT. Counterstain: DAPI (blue) nuclear stain at 1:40000 for 2 hours at RT. Localization: Nucleus. Cytoplasm. Magnification: 20x. (A) DAPI (blue) nuclear stain. (B) Anti-Acetylated Lysine Antibody. (C) Composite. Heat Shocked at 42°C for 1h. | Western blot analysis of Mouse Spleen lysates showing detection of Acetylated Lysine protein using Rabbit Anti-Acetylated Lysine Polyclonal Antibody (SPC-155). Primary Antibody: Rabbit Anti-Acetylated Lysine Polyclonal Antibody (SPC-155) at 1:1000. Rabbit Anti-Acetylated Lysine Antibody used in Immunocytochemistry/Immunofluorescence (ICC/IF) on Human Heat Shocked HeLa Cells (SPC-155) | Rabbit Anti-Acetylated Lysine Antibody used in Western blot (WB) on Bovine serum albumin (SPC-155) | Rabbit Anti-Acetylated Lysine Antibody used in Immunocytochemistry/Immunofluorescence (ICC/IF) on Human Heat Shocked HeLa Cells (SPC-155) | Rabbit Anti-Acetylated Lysine Antibody used in Western blot (WB) on Mouse Spleen lysates (SPC-155)
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Warning information
Non-hazardous
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Country of production
Canada
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Total weight kg
1.4
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Net weight g
0.1
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Stock availabilit
In Stock
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Description
This antibody needs to be stored at + 4°C in a fridge short term in a concentrated dilution. Freeze thaw will destroy a percentage in every cycle and should be avoided. Antibody for research use.
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Group
Polyclonals and antibodies
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About
Polyclonals can be used for Western blot, immunohistochemistry on frozen slices or parrafin fixed tissues. The advantage is that there are more epitopes available in a polyclonal antiserum to detect the proteins than in monoclonal sera. Rabbits are used for polyclonal antibody production by StressMark antibodies. Rabbit antibodies are very stable and can be stored for several days at room temperature. StressMark antibodies adds sodium azide and glycerol to enhance the stability of the rabbit polyclonal antibodies. Anti-human, anti mouse antibodies to highly immunogenic selected peptide sequences are" monoclonal like" since the epitope to which they are directed is less than 35 amino acids long.
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Latin name
Oryctolagus cuniculus
