QuickTiter™ MuLV Core Antigen ELISA test kit

  • Catalog number
    VPK-156
  • Price
    Please ask
  • Size
    96 assays
  • We send this reagant on
    Blue Ice
  • The temperatureerature for storage is
    Upon receipt, aliquot and store the Recombinant MuLV p30 Standard at -20ºC to avoid multiple freeze/thaw cycles. Store all other test kit components at 4ºC.
  • Technical file
    more kit.pdf
  • SDS or safty data sheet
    details (VPK-156) GHS.pdf
  • Type of reagent
    Pathogen and Toxin Assays
  • Related techniques
    Pathogen and Toxin Assays, Virus Core Antigen Detection, MuLV Core Antigen ELISA test kit
  • Use of the reagent
    An anti-MuLV p30 monoclonal coating antibody is adsorbed onto a microtiter plate. MuLV core antigen (p30) present in the sample or standard binds to the antibodies adsorbed on the plate; an anti-MuLV p30 polyclonal antibody is added and binds to the antigen captured by the first antibody. Following incubation and wash steps, a HRP-conjugated secondary antibody is added and binds to the anti-MuLV p30 polyclonal. Unbound HRP-conjugated secondary antibody is removed during the wash steps, and substrate solution reactive with HRP is added to the wells. A colored product is formed in proportion to the amount of MuLV core antigen present in the sample. The reaction is terminated by addition of acid and absorbance is measured at 450 nm. A standard curve is prepared from recombinant MuLV p30 core antigen and sample concentration is then determined.
  • Cellular use
    Measures the MuLV core protein (p30) as low as 300 pg/mL
  • Protocol information
    quantification is performed on a standard microplate reader
  • Reagent properties
    Recombinant MuLV p30 Standard included
  • Living modified organisme in this reagent or LMO
    Recombinant Protein purified from E. coli
  • Species compatible to
    Humans
  • Use of this test kit or product
    Genetics lab
  • Antigen
    a specific protein sequence
  • Buffer
    The concentration in mg/ml in a 10mM of buffered solution at pH 7 to 7,43
  • Molecular weight
    in kilo Danlto or kDa
  • This reagent is purified
    0.97
  • Description
    Antigens are peptides or recombinant or native dependent on the production method.
  • Properties
    E05 478 566 350 170 or Enzyme-Linked Immunosorbent Assays
  • Test
    ELISA Enzyme-linked immunosorbent assays Code 90320007 SNOMED
  • Storage
    Human ELISAs or ELISA kits will be stored at +4°C. The expiry date is mostly determined by the standard stability. If your ELISA kit is expired we can supply a new standard so it will still be functional.
  • Group
    Enzyme-Linked Immunosorbent Assay kits
  • Gene target
    QuickTiter™   MuLV   Core   kit  
  • Short name
    QuickTiter™ MuLV Core Antigen ELISA test kit
  • Technique
    ELISA test, antigen, ELISA, antigenes, ELISAs Enzyme-linked immunosorbent assay 90320007 SNOMED code are used by the medical researcher for detection of human, mouse, … proteins are supplied in coated 96 well plates to be stored at +4°C. ELISA test kits can be sandwich ELISA. In sandwich ELISAS Enzyme-linked immunosorbent assay 9we use captor and detector antibodies. In single antibody ELISAs the antigen is coated and only a detector antibody is used. Traditional competition antigen ELISAs are coated with a captor antibody and a competitive antigen is labelled with the chromogen. In this case the ratio on your graph will be inverse proportional with the antigen present in your sample. A sign detected by the Colorimetric detection OD optical density absorbance at 450 nm between 0 and 4 with an ELISA reader for 450 nm absortion. Genprice Inc. the recognized authority tax ID 45-4304622 D-U-N-S number - 078440800. MeSH code E05 478 566 350 170 Enzyme-Linked Immunosorbent Assay. PROMT code 3841327 Immunoabsorbent assay tests.
  • Host
    antigen assay ELISA kit 1 Kit (96 Wells)
  • Alternative name
    QuickTiter™ MuLV Core protein Enzyme-linked immunosorbent assay test reagent
  • Alternative technique
    antigenes, elisas, tests, kits
  • Alternative to gene target
    v-kit Hardy-Zuckerman 4 feline sarcoma viral oncogene homolog, C-Kit and CD117 and PBT and SCFR, KIT and IDBG-18980 and ENSG00000157404 and 3815, transferase activity, Extracellular, Kit and IDBG-172083 and ENSMUSG00000005672 and 16590, KIT and IDBG-642326 and ENSBTAG00000002699 and 280832
MeSH Data
  • Name
  • Concept
    Scope note: Identification of proteins or peptides that have been electrophoretically separated by blot transferring from the electrophoresis gel to strips of nitrocellulose paper, followed by labeling with antibody probes.
  • Tree numbers
    • E05.196.401.143
    • E05.301.300.096
    • E05.478.566.320.200
    • E05.601.262
    • E05.601.470.320.200
  • Qualifiers
    ethics, trends, veterinary, history, classification, economics, instrumentation, methods, standards, statistics & numerical data
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