QuickDetect™ CD40 Ligand (Human) ELISA Kit

  • Catalog number
    K4414-100
  • Price
    Please ask
  • Size
    100 assays
  • Description
    A rapid sandwich ELISA kit for the quantitative measurement of CD40 Ligand in human serum, plasma, cell culture supernatnat and other biological fluids
  • Summary
    • Detection method- Absorbance (450 nm)• Species reactivity- Human• Application- quantitative measurement of CD40L in human serum, plasma, cell culture supernatant, and other biological fluid.
  • Detection Method
    Absorbance (450 nm)
  • Species Reactivity
    Human
  • Applications
    This ELISA kit is used for quantitative measurement of CD40L in human serum, plasma, cell culture supernatant and other biological fluids.
  • Sample Type
    Human serum, plasma, tissue homogenates and other biological fluids.
  • Features Benefits
    Easy, convenient and time-saving method to measure the level of CD40 Ligand in human serum, plasma, culture supernatants and other biological fluids. The entire assay can be performed within 90 minutes with with fewer incubation and wash step respectively, resulting in less errors and more consistent results. Detection Range: 45 - 4000 pg/ml Sensitivity: < 14 pg/ml Assay Precision: Intra-Assay: CV < 10%; Inter-Assay: CV < 12% (CV (%) = SD/mean X 100) Cross Reactivity: No significant cross-reactivity or interference between this analyte and its analogues was observed.
  • Storage Conditions
    4°C
  • Shipping Conditions
    gel pack
  • Shelf life
    6 months
  • Background
    Cytokine that binds to CD40/TNFRSF5. Costimulates T-cell proliferation and cytokine production. Its cross-linking on T-cells generates a costimulatory signal which enhances the production of IL4 and IL10 in conjunction with the TCR/CD3 ligation and CD28 costimulation. Induces the activation of NF-kappa-B and kinases MAPK8 and PAK2 in T-cells. Induces tyrosine phosphorylation of isoform 3 of CD28. Mediates B-cell proliferation in the absence of co-stimulus as well as IgE production in the presence of IL4. Involved in immunoglobulin class switching. BioVision’s QuickDetect™ CD40 Ligand ELISA kit is a sandwich ELISA assay for measuring the quantity of human CD40 Ligand in serum, plasma and cell culture and other biological fluid. The entire process can be performed within 90 minutes with fewer incubation and wash steps respectively, resulting in less errors and more consistent results.
  • Properties
    E05 478 566 350 170 or Enzyme-Linked Immunosorbent Assays, E05 478 566 350 170 or Enzyme-Linked Immunosorbent Assays, Human proteins, cDNA and human recombinants are used in human reactive ELISA kits and to produce anti-human mono and polyclonal antibodies. Modern humans (Homo sapiens, primarily ssp. Homo sapiens sapiens). Depending on the epitopes used human ELISA kits can be cross reactive to many other species. Mainly analyzed are human serum, plasma, urine, saliva, human cell culture supernatants and biological samples.
  • Test
    ELISA Enzyme-linked immunosorbent assays Code 90320007 SNOMED
  • Additional description
    FAS ligand and other ligands are binding to the receptor for signaling pathways for example in apoptosis or JNK signaling. Receptor agonists are often tested for drug development.
  • Gene target
  • Gene symbol
    CD40
  • Short name
    QuickDetect™ CD40 Ligand ( ) ELISA Kit
  • Technique
    ELISA kit, ELISA, ELISAs Enzyme-linked immunosorbent assay 90320007 SNOMED code are used by the medical researcher for detection of human, mouse, … proteins are supplied in coated 96 well plates to be stored at +4°C. ELISA test kits can be sandwich ELISA. In sandwich ELISAS Enzyme-linked immunosorbent assay 9we use captor and detector antibodies. In single antibody ELISAs the antigen is coated and only a detector antibody is used. Traditional competition antigen ELISAs are coated with a captor antibody and a competitive antigen is labelled with the chromogen. In this case the ratio on your graph will be inverse proportional with the antigen present in your sample. A sign detected by the Colorimetric detection OD optical density absorbance at 450 nm between 0 and 4 with an ELISA reader for 450 nm absortion. Genprice Inc. the recognized authority tax ID 45-4304622 D-U-N-S number - 078440800. MeSH code E05 478 566 350 170 Enzyme-Linked Immunosorbent Assay. PROMT code 3841327 Immunoabsorbent assay tests. ELISA tests are enzyme linked immunoassays to detect human, mouse or other proteins in serum, plasma, urine or biological fluids. MeSH code E05 478 566 350 170 for Enzyme-Linked Immunosorbent Assay or ELISA kits. Also Enzyme-linked immunoassay, competitive have the SNOMED name 61461007 and the PROMT code for ELISA readers is 3841330. This is the desigation of other diagnostic analyzers and tests.
  • Host
    antigen assay ELISA kit 1 Kit (96 Wells)
  • Species
    Humans
  • Alternative name
    QuickDetect™ CD40 molecule, tumor necrosis factor receptor superfamily member 5 Ligand (H. sapiens) Enzyme-linked immunosorbent assay reagent
  • Alternative technique
    elisas, kits
  • Alternative to gene target
    v-kit Hardy-Zuckerman 4 feline sarcoma viral oncogene homolog, C-Kit and CD117 and PBT and SCFR, KIT and IDBG-18980 and ENSG00000157404 and 3815, transferase activity, Extracellular, Kit and IDBG-172083 and ENSMUSG00000005672 and 16590, KIT and IDBG-642326 and ENSBTAG00000002699 and 280832
Gene info
MeSH Data
  • Name
  • Concept
    Scope note: The number of CD4-POSITIVE T-LYMPHOCYTES per unit volume of BLOOD. Determination requires the use of a fluorescence-activated flow cytometer.
  • Tree numbers
    • E01.370.225.500.195.107.595.500.150
    • E01.370.225.625.107.595.500.150
    • E05.200.500.195.107.595.500.150
    • E05.200.625.107.595.500.150
    • E05.242.195.107.595.500.150
  • Qualifiers
    ethics, trends, veterinary, history, classification, economics, instrumentation, methods, standards, statistics & numerical data
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