pVision-GFP-N Vector

• Catalog number
  9999-20
• Price
  Please ask
• Size
  20 μg

• Description
  An eukaryotic expression vector encoding green fluorescent protein. pVisionGFP-N1 vector is a mammalian expression vector encoding green fluorescent protein, VisionGFP. pVisionGFP-N vector is designed to generate fusions to VisionGFP N-terminus for expression, localization and cellular dynamics studies or to express VisionGFP in eukaryotic (mammalian) cells. pVisionGFP-N vector carries synthetic version of the VisionGFP gene which codon usage is humanized, i.e. optimized for high expression in mammalian cells. pVisionGFP-N vector backbone contains immediate early promoter of cytomegalovirus (PCMV IE) for protein expression, SV40 origin for replication in mammalian cells, pUC origin of replication for propagation in E. coli, and f1 origin for single-stranded DNA production. SV40 early promoter provides neomycin resistance gene expression to select stably transfected eukaryotic cells using G418. Bacterial promoter (P) provides kanamycin resistance gene expression in E. coli. To increase VisionGFP mRNA translation efficiency, Kozak consensus translation initiation site is generated upstream of VisionGFP coding sequence. Multiple cloning site (MCS) is located between PCMV IE and VisionGFP coding sequence.
• Product Highlights
  • Amount - 20 µg • Application: N-terminal mammalian expression vector encoding humanized TagGFP and allowing TagGFP expression and generation of fusions to the TagGFP N-terminus. Generation of Vision GFP-tagged fusions A localization signal or a gene of interest should be cloned into MCS of the vector. It will be expressed as a fusion to VisionGFP N-terminus when inserted in the same reading frame as VisionGFP and no in-frame stop codons are present. VisionGFP-tagged fusions retain fluorescent properties of the native protein allowing fusion localization in vivo. Unmodified pVisionGFP-N vector will express VisionGFP when transfected into eukaryotic (mammalian) cells. Expression in Mammalian Cells pVisionGFP-N can be transfected into mammalian cells by any known transfection method. CMV promoter provides strong, constitutive expression of VisionGFP or VisionGFP-tagged fusions in many cell types. If required, stable transformants can be selected using G418. Propagation in E. coli • Suitable host strains: DH5alpha, HB101, and other general purpose strains. Single-stranded DNA production requires a host containing an F plasmid such as JM109 or XL1-Blue. • Selectable marker: plasmid confers resistance to kanamycin (30 μg/ml) to E. coli hosts. • E. coli replication origin: pUC • Copy number: ~500 • Plasmid incompatibility group: pMB1/ColE1 Generation of Vision GFP-tagged fusions A localization signal or a gene of interest should be cloned into MCS of the vector. It will be expressed as a fusion to VisionGFP N-terminus when inserted in the same reading frame as VisionGFP and no in-frame stop codons are present. VisionGFP-tagged fusions retain fluorescent properties of the native protein allowing fusion localization in vivo. Unmodified pVisionGFP-N vector will express VisionGFP when transfected into eukaryotic (mammalian) cells. Expression in Mammalian Cells pVisionGFP-N can be transfected into mammalian cells by any known transfection method. CMV promoter provides strong, constitutive expression of VisionGFP or VisionGFP-tagged fusions in many cell types. If required, stable transformants can be selected using G418. Propagation in E. coli -Suitable host strains: DH5alpha, HB101, and other general purpose strains. Single-stranded DNA production requires a host containing an F plasmid such as JM109 or XL1-Blue. -Selectable marker: plasmid confers resistance to kanamycin (30 μg/ml) to E. coli hosts. - E. coli replication origin: pUC -Copy number: ~500 -Plasmid incompatibility group: pMB1/ColE1
• Storage Temp
  -20°C
• Shipping
  gel pack
• Shelf Life
  12 months
• Datasheet
• Notes
  For research use only. Not to be used for human or animal treatment or consumption.

• Gene target
  pVision-GFP-N   Vector  
• Gene symbol
  GH1, POLM, NRAS, SDC3, PIGN, WASL, MAP6, HLA-N, FERD3L, SHC3
• Short name
  pVision-GFP-N Vector
• Technique
  Vectors
• Label
  gfp
• Alternative name
  pVision-Green Fluorescent-N integrating Desoxyribonucleic acid sequence

Gene info

• Identity
  HGNC:4261
• Gene
  GH1
• Long gene name
  growth hormone 1
• Synonyms
  • GH-N
  • GHN
  • GH
  • hGH-N
• Synonyms name
  • pituitary growth hormone
  • somatotropin
• GenBank acession
  • M13438
• Locus
  17q23.3
• Discovery year
  2001-06-22
• Entrez gene record
  2688
• Pubmed identfication
  • 6306568
• RefSeq identity
  • NM_000515
• Classification
  • Growth hormone family
• VEGA ID
  OTTHUMG00000172293

Gene info

• Identity
  HGNC:9185
• Gene
  POLM
• Long gene name
  DNA polymerase mu
• Synonyms gene name
  • polymerase (DNA directed), mu
  • polymerase (DNA) mu
• Synonyms
  • Tdt-N
• Synonyms name
  • Pol iota
• GenBank acession
  • AF176097
• Locus
  7p13
• Discovery year
  2000-02-16
• Entrez gene record
  27434
• Pubmed identfication
  • 10982892
• RefSeq identity
  • NM_013284
• Classification
  • DNA polymerases
  • MicroRNA protein coding host genes
• VEGA ID
  OTTHUMG00000155353

Gene info

• Identity
  HGNC:7989
• Gene
  NRAS
• Long gene name
  NRAS proto-oncogene, GTPase
• Synonyms gene name
  • neuroblastoma RAS viral (v-ras) oncogene homolog
  • neuroblastoma RAS viral oncogene homolog
• Synonyms
  • N-ras
• GenBank acession
  • BC005219
• Locus
  1p13.2
• Discovery year
  2001-06-22
• Entrez gene record
  4893
• Pubmed identfication
  • 6705568
  • 11524732
• RefSeq identity
  • NM_002524
• Classification
  • RAS type GTPase family
• VEGA ID
  OTTHUMG00000012059
• Locus Specific Databases
  • NRASbase: Mutation registry for autoimmune lymphoproliferative syndrome type IV
  • LRG_92

Gene info

• Identity
  HGNC:10660
• Gene
  SDC3
• Long gene name
  syndecan 3
• Synonyms gene name
  • syndecan 3 (N-syndecan)
• Synonyms
  • N-syndecan
  • SYND3
• Synonyms name
  • syndecan proteoglycan 3
• GenBank acession
  • AF248634
• Locus
  1p35.2
• Discovery year
  1994-05-16
• Entrez gene record
  9672
• Pubmed identfication
  • 1556152
  • 11527150
• RefSeq identity
  • NM_014654
• Classification
  • Syndecans
• VEGA ID
  OTTHUMG00000043646

Gene info

• Identity
  HGNC:8967
• Gene
  PIGN
• Long gene name
  phosphatidylinositol glycan anchor biosynthesis class N
• Synonyms gene name
  • phosphatidylinositol glycan, class N
• Synonyms
  • MCD4
  • PIG-N
• GenBank acession
  • AF109219
• Locus
  18q21.33
• Discovery year
  2000-05-11
• Entrez gene record
  23556
• Pubmed identfication
  • 10069808
  • 10574991
• RefSeq identity
  • NM_176787
• Classification
  • Phosphatidylinositol glycan anchor biosynthesis
• VEGA ID
  OTTHUMG00000180098

Gene info

• Identity
  HGNC:12735
• Gene
  WASL
• Long gene name
  WASP like actin nucleation promoting factor
• Synonyms gene name
  • Wiskott-Aldrich syndrome-like
  • Wiskott-Aldrich syndrome like
• Synonyms
  • N-WASP
  • NWASP
  • WASPB
• GenBank acession
  • D88460
• Locus
  7q31.32
• Discovery year
  1998-10-14
• Entrez gene record
  8976
• Pubmed identfication
  • 9422512
  • 9322739
  • 23527602
• RefSeq identity
  • NM_003941
• Classification
  • Wiskott-Aldrich Syndrome protein family
• VEGA ID
  OTTHUMG00000157346

Gene info

• Identity
  HGNC:6868
• Gene
  MAP6
• Long gene name
  microtubule associated protein 6
• Synonyms
  • KIAA1878
  • STOP
  • FLJ41346
  • MAP6-N
• GenBank acession
  • AK123340
• Locus
  11q13.5
• Discovery year
  1998-02-26
• Entrez gene record
  4135
• Pubmed identfication
  • 10516426
  • 12231625
• RefSeq identity
  • NM_033063
• VEGA ID
  OTTHUMG00000165343

Gene info

• Identity
  HGNC:19406
• Gene
  HLA-N
• Long gene name
  major histocompatibility complex, class I, N (pseudogene)
• Synonyms
  • HLA-30
• GenBank acession
  • M96329
• Locus
  6p22.1
• Discovery year
  2002-10-23
• Entrez gene record
  267014
• Pubmed identfication
  • 10452889
  • 1517563
• RefSeq identity
  • NG_009940
• Classification
  • Histocompatibility complex
• VEGA ID
  OTTHUMG00000031167
• Locus Specific Databases
  • IMGT/HLA Database

Gene info

• Identity
  HGNC:16660
• Gene
  FERD3L
• Long gene name
  Fer3 like bHLH transcription factor
• Synonyms gene name
  • Fer3-like (Drosophila)
  • Fer3-like bHLH transcription factor
• Synonyms
  • NATO3
  • N-TWIST
  • bHLHa31
• GenBank acession
  • AF369897
• Locus
  7p21.1
• Discovery year
  2002-12-09
• Entrez gene record
  222894
• Pubmed identfication
  • 11472856
  • 12217327
• Classification
  • Basic helix-loop-helix proteins
• VEGA ID
  OTTHUMG00000090823

Gene info

• Identity
  HGNC:18181
• Gene
  SHC3
• Long gene name
  SHC adaptor protein 3
• Synonyms gene name
  • src homology 2 domain containing transforming protein C3
  • SHC (Src homology 2 domain containing) transforming protein 3
• Synonyms
  • N-Shc
  • NSHC
  • SHCC
• GenBank acession
  • D84361
• Locus
  9q22.1
• Discovery year
  2003-11-12
• Entrez gene record
  53358
• Pubmed identfication
  • 8808684
• RefSeq identity
  • NM_016848
• Classification
  • SH2 domain containing
• VEGA ID
  OTTHUMG00000020179

MeSH Data

• Name
  Transduction, Genetic
• Concept
  Scope note: The transfer of bacterial DNA by phages from an infected bacterium to another bacterium. This also refers to the transfer of genes into eukaryotic cells by viruses. This naturally occurring process is routinely employed as a GENE TRANSFER TECHNIQUE.
• Tree numbers
  • E05.393.350.800
• Qualifiers
  ethics, trends, veterinary, history, classification, economics, instrumentation, methods, standards, statistics & numerical data

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