Puromycin DiHCl, 10 mg/ml in 20 mM HEPES, 1 ml

  • Catalog number
    P025-5x1ml
  • Price
    Please ask
  • Size
    5 x 1 ml
  • Chemical formula
    C22H29N7O5*2HCl
  • CAS number
    58-58-2
  • Percentage purity or assay grade
    N/A
  • Ordering information
    If you wish to order this item, please send us by email or by fax an official purchase order including your full shipping and billing information. Contact us if you need first a quotation. Delivery for USA and Europe next week if an order is received before Friday 2 pm.
  • Shipping condition
    dry ice
  • Storage condition
    -20°C
  • Buffer
    25mM HEPES (4-(2-hydroxyethyl)-1-piperazineethanesulfonic acid ) pH7 solution buffers are a media with a zwitterion organic chemical buffering agent; one of the twenty Good's buffers. HEPES DMEM medium is widely used in cell culture, largely because it is better at maintaining physiological pH 7 tot 7,5 despite changes in carbon dioxide concentration (produced by cellular respiration) when compared to bicarbonate buffers, which are also commonly used in cell culture. The dissociation of water decreases with falling temperature, but the dissociation constants (pK) of many other buffers do not change much with temperature. HEPES, 25 mM in RPMI and DMEM is like water in that its dissociation decreases as the temperature decreases. This makes HEPES RPMI 25 milli-molar media with or without L-glutamine a more effective buffering agent for maintaining enzyme structure and function at low temperatures.
  • Gene target
  • Short name
    Puromycin DiHCl, 10 mg/ in 20 mM , 1
  • Alternative name
    Puromycin DiHCl, 10 mg/milliliter in 20 millimeter HEPES, 1 milliliter
MeSH Data
  • Name
  • Concept
    Scope note: Histochemical localization of immunoreactive substances using labeled antibodies as reagents.
  • Tree numbers
    • E01.370.225.500.607.512
    • E01.370.225.750.551.512
    • E05.200.500.607.512
    • E05.200.750.551.512
    • E05.478.583
  • Qualifiers
    ethics, trends, veterinary, history, classification, economics, instrumentation, methods, standards, statistics & numerical data
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