PurKine Biotin-Tag Protein Purification Kit (Streptavidin)

Application

protein purification
Shipping conditions

The product PurKine Biotin-Tag Protein Purification Kit (Streptavidin) must be shipped on blue ice packages to retain the quality and activity of the reagents during the transportation.
Storage conditions

The product PurKine Biotin-Tag Protein Purification Kit (Streptavidin) can be kept between four and eight degrees Celsius for 12 months upon receipt.
Tips

This kit is manufactured for research purposes only and it is not intended to be used in clinical diagnostic procedures.
Properties

Biotin conjugates can be detected by horseradish peroxidase, alkaline phosphatase substrates or anti biotin conjugated antibodies. Avidin and Streptavidin bind to the small biotin and are couple to HRP or AP for ELISA. To break the streptavidin Biotin bond we suggest to use a 6 molar guanidine HCl solution with acidity of pH 1.6.
Conjugation

Biotinylated
Test

Antibodies are affinity purified with an antigen coated column or protein A or G agarose or beads. DNA is purified with endotoxin free silica columns or anion exchange resins. Abbkine supplies purification kits and ultra pure reagents.

Gene target

PurKine Tag Protein Kit Streptavidin
Short name

PurKine -Tag Protein Purification Kit (Streptavidin)
Technique

Purification, purified, Tags
Label

Biotin
Alternative name

PurKine biotinilated-detection labelled Protein purification reagent (biotinilated-binding streptavidin)
Alternative technique

purifications, kits
Alternative to gene target

v-kit Hardy-Zuckerman 4 feline sarcoma viral oncogene homolog, C-Kit and CD117 and PBT and SCFR, KIT and IDBG-18980 and ENSG00000157404 and 3815, transferase activity, Extracellular, Kit and IDBG-172083 and ENSMUSG00000005672 and 16590, KIT and IDBG-642326 and ENSBTAG00000002699 and 280832