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Available ordering format
Lyophilized
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Immunogen
KLH-conjugated peptide chosen chosen from Arabidopsis thaliana PsbO1, UniProt: P23321, TAIR:At5g66570
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Raised in
Rabbit
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Clonality
Polyclonal
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Clone
Polyclonal
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Purification
Serum
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How to reconstitute
For reconstitution add 50 µl of sterile water.
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Storage condition
store lyophilized/reconstituted at -20°C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please, remember to spin tubes briefly prior to opening them to avoid any losses that might occur from lyophilized material adhering to the cap or sides of the tubes.
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Verified applications
western blot (WB), immunoprecipitation (IP)
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Connected products
AS14 2825 | anti-PsbO2 | 33 kDa of the oxygen evolving complex (OEC) of PSII, rabbit antibodies AS05 092 | anti-PsbO | 33 kDa of the oxygen evolving complex (OEC) of PSII, rabbit antibodyAS06 142-33 | anti-PsbO | 33 kDa of the oxygen evolving complex (OEC) of PSII, rabbit antibodyAS06 167 | anti-PsbP | 23 kDa protein of the oxygen evolving complex (OEC) of PSII, rabbit antibodyAS08 305 | anti-PsbP | 23 kDa protein of the oxygen evolving complex (OEC) of PSII, rabbit antibodyAS06 142-16 | anti-PsbQ | 16 kDa protein of the oxygen evolving complex (OEC) of PSII, rabbit antibodyPlant protein extraction buffer Secondary antibodies
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Recommended dilutions for use
1: 1000 with standard ECL (WB)
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Molecular weight expected аpparent
33 | 30 kDa
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Verified reactivity
Arabidopsis thaliana
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Possible reactivity
Brasica napus, Capsella rubella, Triticum aestivum
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No reactivity
Oryza sativa
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Supplementary information
This antibody is specific to PsbO1 and does not react with PsbO2 as confirmed on a deletion mutant.
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References
to be added when available, antibody released in January 2015
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Scientific context
The PsbO protein is an extrinisic subunit of the water splitting photosystem II (PSII) complex. The protein is exposed on the luminal side of the thylakoid membrane, and is hihgly conserved in all known oxygenic photosynthetic organisms. Alternative names of PsbO1 include 33 kDa subunit of oxygen evolving system of photosystem II, OEC 33 kDa subunit, 33 kDa thylakoid membrane protein, manganese-stabilizing protein 1 and for PsbO2 33 kDa subunit of oxygen evolving system of photosystem II, OEC 33 kDa subunit, 33 kDa thylakoid membrane protein, manganese-stabilizing protein 2.
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Notes
None
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Protein number
P23321
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TAIR number
At5g66570
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Description
The kilo Daltons subunit weight of PsbO1, 33 of the oxygen evolving complex (OEC) of PSII compared to your protein ladder can be shifted a little due to electrophoresis effects. 1 kDa = 1000 g/mol protein
