PsbF, beta subunit of Cytochrome b559 of PSII

• Catalog number
  AS06 113
• Price
  Please ask
• Size
  200 µl

• Available ordering format
  Lyophilized
• Immunogen
  KLH-conjugated synthetic peptide derived from plant PsbF sequences including Arabidopsis thaliana P62095, AtCg00570
• Raised in
  Rabbit
• Clonality
  Polyclonal
• Clone
  Polyclonal
• Purification
  Serum
• How to reconstitute
  For reconstitution add 200 µl of sterile water.
• Storage condition
  store lyophilized/reconstituted at -20°C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please, remember to spin tubes briefly prior to opening them to avoid any losses that might occur from lyophilized material adhering to the cap or sides of the tubes.
• Verified applications
  Western blot (WB)
• Connected products
  collection of antibodies to PSII proteins Plant protein extraction buffer Secondary antibodies
• Recommended dilutions for use
  1: 1000 (WB)
• Molecular weight expected аpparent
  4 kDa
• Verified reactivity
  Arabidopsis thaliana, Pisum sativum
• Possible reactivity
  Nicotiana tabacum, Pinus sylvestris, Populus tremula,
• No reactivity
  Cyanobacteria
• Supplementary information
  This antibody works better on thylakoid and chloroplast fractions than on a total cell extract.
• References
  Nishimura et al. (2016). The N-terminal sequence of the extrinsic PsbP protein modulates the redox potential of Cyt b559 in photosystem II. Sci Rep. 2016 Feb 18;6:21490. doi: 10.1038/srep21490.Lucinski et al. (2011). Involvement of Deg5 protease in wounding-related disposal of PsbF apoprotein. Plant Physiol Biochem. 49(3):311-20.Garcia-Cerdan et al. (2008). Antisense inhibition of the PsbX protein affects PSII integrity in the higher plant Arabidopsis thaliana. Plant Cell Physiol 50: 191-202
• Scientific context
  Cytochrome b559 (Cyt b559) is encoded by the chloroplast genes psbE and psbF and is comprised of two low molecular mass polypeptides, α and ß subunits, with molecular masses of 9 and 4 kDa, respectively. The Cyt b559 is closely associated with PSII in all oxygenic photosynthetic organisms. The α and ß subunits of the Cyt b559 are components of the minimal PSII reaction center complex that is still capable of primary charge separation In summary, both PsbE and PsbF are essential components for PSII assembly, and they are probably involved in electron transport mechanisms that help to protect PSII from photodamage.
• Notes
  None
• Protein number
  P62095
• TAIR number
  ATCG00570
• Hyperlink

• Gene target
  PsbF   beta   subunit   Cytochrome   b559   PSII  
• Short name
  PsbF, beta subunit of Cytochrome b559 of PSII
• Alternative name
  PsbF, b functionnal sequence on Cytochrome b559 on PSII

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