PsbE, alfa subunit of Cytochrome b559 of PSII

  • Catalog number
    AS06 112
  • Price
    Please ask
  • Size
    100 µl
  • Available ordering format
    Lyophilized
  • Immunogen
    KLH-conjugated synthetic peptide chosen from PsbE protein of Arabidopsis thaliana P56779, AtCg00580
  • Raised in
    Rabbit
  • Clonality
    Polyclonal
  • Clone
    Polyclonal
  • Purification
    Serum
  • How to reconstitute
    For reconstitution add 100 µl of sterile water.
  • Storage condition
    store lyophilized/reconstituted at -20°C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please, remember to spin tubes briefly prior to opening them to avoid any losses that might occur from lyophilized material adhering to the cap or sides of the tubes.
  • Verified applications
    Western blot (WB)
  • Connected products
    collection of antibodies to PSII proteinsPlant protein extraction buffer Secondary antibodies
  • Recommended dilutions for use
    1 : 5000 with standard ECL (WB)
  • Molecular weight expected аpparent
    9.25 kDa
  • Verified reactivity
    Arabidopsis thaliana, Hordeum vulgare, Nicotiana tabacum, Spinacia oleracea
  • Possible reactivity
    dicots including Glycine max, Salvia miltiorrhiza, Solanum tuberosum, deciduous flowering plant Populus alba
  • No reactivity
    Chlamydomonas reinhardtii, Synechococcus sp. PCC 7942
  • Supplementary information
    to be added when available
  • References
    Nishimura et al. (2016). The N-terminal sequence of the extrinsic PsbP protein modulates the redox potential of Cyt b559 in photosystem II. Sci Rep. 2016 Feb 18;6:21490. doi: 10.1038/srep21490. Grieco et al. (2015). Light-harvesting II antenna trimers connect energetically the entire photosynthetic machinery - including both photosystems II and I. Biochim Biophys Acta. 2015 Jun-Jul;1847(6-7):607-19. doi: 10.1016/j.bbabio.2015.03.004. Epub 2015 Apr 3. Hojka et al. (2014). Inducible repression of nuclear-encoded subunits of the cytochrome b6f complex in tobacco reveals an extraordinarily long lifetime of the complex. Plant Physiol. 2014 Jun 24. pii: pp.114.243741.
  • Scientific context
    Cytochrome b559 (Cyt b559) is encoded by the chloroplast genes psbE and psbF and is comprised of two low molecular mass polypeptides, a and h subunits, with molecular masses of 9 and 4 kDa, respectively. The Cyt b559 is closely associated with PSII in all oxygenic photosynthetic organisms. The a and h subunits of the Cyt b559 are components of the minimal PSII reaction center complex that is still capable of primary charge separation In summary, both PsbE and PsbF are essential components for PSII assembly, and they are probably involved in electron transport mechanisms that help to protect PSII from photodamage.Altrnative protein name: PSII reaction center subunit V
  • Notes
    cellular [compartment marker] of thylakoid membrane
  • Protein number
    P56779
  • TAIR number
    ATCG00580
  • Gene target
    PsbE   alfa   subunit   Cytochrome   b559   PSII  
  • Short name
    PsbE, alfa subunit of Cytochrome b559 of PSII
  • Alternative name
    PsbE, alfa functionnal sequence on Cytochrome b559 on PSII
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