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Available ordering format
Lyophilized in PBS pH 7.4
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Immunogen
KLH-conjugated peptide derived from known sequences of Psb29 including Arabidopsis thaliana Q9SKT0, At2g20890
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Raised in
Rabbit
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Clonality
Polyclonal
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Clone
Polyclonal
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Purification
Affinity purified serum
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How to reconstitute
For reconstitution add 100 µl of sterile water.
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Storage condition
store lyophilized/reconstituted at -20°C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please, remember to spin tubes briefly prior to opening them to avoid any losses that might occur from lyophilized material adhering to the cap or sides of the tubes.
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Verified applications
Western blot (WB), immunoprecipitation (IP)
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Connected products
collection of antibodies to proteins involved in photosynthesis
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Recommended dilutions for use
1: 10 000 with standard ECL (WB), 5 µg in 300 µl (IP)
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Molecular weight expected аpparent
33.7 | 22 kDa
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Verified reactivity
Arabidopsis thaliana, Hordeum vulgare, Nicotiana benthamiana
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Possible reactivity
dicots including:Nicotiana tabacum,Solanum tuberosum, monocots including: Oryza sativa, trees: Picea sitchensis, Populus balsamifera, moss: Physcomitrella patens, algae: Ostreococcus sp.
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No reactivity
no confirmed exceptions from predicted reactivity known in the moment
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Supplementary information
to be added when available
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References
Hamel et al. (2016). The chloroplastic protein THF1 interacts with the coiled-coil domain of the disease resistance protein N' and regulates light-dependent cell death. Plant Physiol. 2016 Mar 7. pii: pp.00234.2016Huang et al. (2013). Arabidopsis Thylakoid Formation 1 Is a Critical Regulator for Dynamics ofPSII-LHCII Complexes in Leaf Senescence and Excess Light. Mol Plant. May 13.
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Scientific context
Psb29 (THF1) is a conserved 22 kDa protein which functions in biogenesis of photosystem II complexes. This protein is required for organization of vesicles into mature thylakoid stacks for chloroplast development. Mediates G-protein signalling between the plasma membrane and the plastid.
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Notes
None
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Protein number
Q9SKT0
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TAIR number
AT2G20890
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Description
Associated membrane protein types are lipopolysaccharide selective barriers. Biological membranes include cell membranes, outer coverings of cells or organelles that allow passage of certain proteins and nuclear membranes, which cover a cell nucleus; and tissue membranes, such as mucosae and serosae.
