Protein Disulfide Isomerase (PDI) Activity Assay Kit (Fluorometric)
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Catalog numberK949-100
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PricePlease ask
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Size100 assays
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Summary• Detection method- Fluorescence (Ex/Em= 490/480 nm) • Species reactivity- Mammalian • Application- This assay has a detection sensitivity as low as 0.1 mU.
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Detection MethodFluorescence (Ex/Em= 490/480 nm)
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Species ReactivityMammalian
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ApplicationsThis assay has a detection sensitivity as low as 0.1 mU.
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Sample TypeCell and tissue culture lysates and purified enzyme preparations.
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Features Benefits• Simple procedure • Fast and convenient
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Storage Conditions-20°C
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Shipping Conditionsgel pack
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Shelf life12 months
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BackgroundProtein Disulfide Isomerases (PDI, EC: 5.3.4.1) are multifunctional proteins that constitute a thio-disulfide oxidoreductase family. PDI is abundant in the lumen of endoplasmic reticulum (ER). PDI plays an essential role in catalyzing the rearrangement of disulfide (S-S) bonds in proteins and functions as a chaperone by inhibiting protein aggregation. Recent studies show that PDI activity is essential for cancer cell survival and proliferation, and targeting PDI activity with specific inhibitors abrogates survival responses leading to ER stress in cancer cells. Therefore, the detection of PDI activity is critical for mechanistic study, diagnosis, prevention, and therapeutic purposes. BioVision’s PDI Activity Assay kit utilizes the reductase activity PDI for determining its activity in a variety of samples. The Kit employs a synthetic quenched fluorescent substrate which gets cleaved in the presence of PDI producing an enhanced fluorometric signal (Ex/Em = 490/580 nm). In the presence of BioVision’s PDI specific inhibitor, the quenched fluorescent substrate cannot be cleaved by PDI. BioVision’s PDI Activity Assay Kit is simple, sensitive, and high-throughput adaptable with the detection limit as low as 0.1 mU.
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TestBiovision supplies other types of Assays as 1.
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Gene target
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Gene symbolPDIA2, PADI1, ERP29, P4HB
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Short nameProtein Disulfide Isomerase (PDI) Activity Assay Kit (Fluorometric)
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TechniqueAssay, assays
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HostAssay
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Alternative nameProtein Disulfide Isomerase (PDI) Activity test reagent (fluorescence)
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Alternative techniquearrays, kits
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Alternative to gene targetv-kit Hardy-Zuckerman 4 feline sarcoma viral oncogene homolog, C-Kit and CD117 and PBT and SCFR, KIT and IDBG-18980 and ENSG00000157404 and 3815, transferase activity, Extracellular, Kit and IDBG-172083 and ENSMUSG00000005672 and 16590, KIT and IDBG-642326 and ENSBTAG00000002699 and 280832
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Gene info
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Identity
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Gene
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Long gene nameprotein disulfide isomerase family A member 2
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Synonyms gene
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Synonyms gene name
- protein disulfide isomerase, pancreatic
- protein disulfide isomerase-associated 2
- protein disulfide isomerase family A, member 2
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Synonyms
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GenBank acession
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Locus
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Discovery year2000-12-21
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Entrez gene record
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Pubmed identfication
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RefSeq identity
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Classification
- Protein disulfide isomerases
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VEGA ID
Gene info
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Identity
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Gene
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Long gene namepeptidyl arginine deiminase 1
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Synonyms gene name
- peptidyl arginine deiminase, type I
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Synonyms
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Synonyms name
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GenBank acession
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Locus
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Discovery year2002-03-12
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Entrez gene record
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Pubmed identfication
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RefSeq identity
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Classification
- Peptidyl arginine deiminases
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VEGA ID
Gene info
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Identity
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Gene
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Long gene nameendoplasmic reticulum protein 29
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Synonyms gene
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Synonyms gene name
- chromosome 12 open reading frame 8
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Synonyms
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Synonyms name
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GenBank acession
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Locus
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Discovery year2001-04-27
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Entrez gene record
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Pubmed identfication
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Classification
- Protein disulfide isomerases
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VEGA ID
Gene info
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Identity
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Gene
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Long gene nameprolyl 4-hydroxylase subunit beta
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Synonyms gene
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Synonyms gene name
- procollagen-proline, 2-oxoglutarate 4-dioxygenase (proline 4-hydroxylase), beta polypeptide (protein disulfide isomerase; thyroid hormone binding protein p55)
- procollagen-proline, 2-oxoglutarate 4-dioxygenase (proline 4-hydroxylase), beta polypeptide (protein disulfide isomerase-associated 1)
- procollagen-proline, 2-oxoglutarate 4-dioxygenase (proline 4-hydroxylase), beta polypeptide
- prolyl 4-hydroxylase, beta polypeptide
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Synonyms
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Synonyms name
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GenBank acession
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Locus
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Discovery year1986-01-01
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Entrez gene record
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Pubmed identfication
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RefSeq identity
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Classification
- Protein disulfide isomerases
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VEGA ID