Prostatic acid phosphatase, human recombinant
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Catalog numberP1066-50
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PricePlease ask
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Size50 ug
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SynonymsPAP, 5'-nucleotidase, ACPP, 5’-NT, ACP-3, ACP3
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Alternative_namesPAP, 5'-nucleotidase, ACPP, 5’-NT, ACP-3, ACP3
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RecombinantYes
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SourceBaculovirus
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Purity by SDS PAGE> 95%
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AssaySDS-PAGE
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Endotoxin Level< 1.0 EU per/ug
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Activity Specifications test methodMeasured by its ability to hydrolyze 1.0 nmoles of p-nitrophenyl phosphate (pNPP) per minute at pH 5.0 at 37C
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Biological activitySpecific activity is >100,000 units/mg, and is defined as the amount of enzyme that hydrolyze 1.0 nmoles of p-nitrophenyl phosphate (pNPP) per minute at pH 5.0 at 37C
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Results>100,000 units/mg
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Unit DefinitionOne unit is defined as the amount of enzyme that hydrolyze 1.0 nmoles of p-nitrophenyl phosphate (pNPP) per minute at pH 5.0 at 37C
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Molecular Weight41.8 kDa
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Storage Temp-20°C
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ShippingGel Pack
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Shelf Life12 months
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Concentration0.5 mg/ml
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AppearanceLiquid
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Physical form descriptionIn Phosphate Buffered Saline (pH 7.4) containing 10% glycerol
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Background InformationACPP, also known as prostatic acid phosphatase isoform PAP, is a type I integral membrane protein of the plasma membrane and lysosomes, and a secreted form also exists. The concentration of ACPP is elevated in the circulation of prostate cancer patients, making the enzyme a marker for the progression of prostate cancer. Recombinant human ACPP, fused to His-tag at C-terminus, was expressed in insect cell and purified by using conventional chromatography techniques.
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Amino acid sequenceKELKFVTLVF RHGDRSPIDT FPTDPIKESS WPQGFGQLTQ LGMEQHYELG EYIRKRYRKF LNESYKHEQV YIRSTDVDRT LMSAMTNLAA LFPPEGVSIW NPILLWQPIP VHTVPLSEDQ LLYLPFRNCP RFQELESETL KSEEFQKRLH PYKDFIATLG KLSGLHGQDL FGIWSKVYDP LYCESVHNFT LPSWATEDTM TKLRELSELS LLSLYGIHKQ KEKSRLQGGV LVNEILNHMK RATQIPSYKK LIMYSAHDTT VSGLQMALDV YNGLLPPYAS CHLTELYFEK GEYFVEMYYR NETQHEPYPL MLPGCSPSCP LERFAELVGP VIPQDWSTEC MTTNSHQGTE DSTDHHHHHH
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HandlingCentrifuge the vial prior to opening.
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UsageFor Research Use Only! Not to be used in humans
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PropertiesHuman proteins, cDNA and human recombinants are used in human reactive ELISA kits and to produce anti-human mono and polyclonal antibodies. Modern humans (Homo sapiens, primarily ssp. Homo sapiens sapiens). Depending on the epitopes used human ELISA kits can be cross reactive to many other species. Mainly analyzed are human serum, plasma, urine, saliva, human cell culture supernatants and biological samples.
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Additional sourceRecombinants or rec. proteins
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Grouprecombinants
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Gene target
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Gene symbolACP3
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Short nameProstatic acid phosphatase, recombinant
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TechniqueRecombinant, E. coli recombinant proteins are genetic recombinations in Escherichia coli, supplied as white sterile powder lyopillized. Biovision advises they will be reconstituted in a buffer soluion or culture medium for cell culture.
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SpeciesHuman, Humans
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Alternative nameProstatic acid phosphatase, H. sapiens Rec.
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Alternative techniquerec
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Gene info
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Identity
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Gene
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Long gene nameacid phosphatase 3
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Synonyms gene
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Synonyms gene name
- acid phosphatase, prostate
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Synonyms
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Synonyms name
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Locus
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Discovery year1989-05-30
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Entrez gene record
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Pubmed identfication
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RefSeq identity
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Classification
- Acid phosphatases
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VEGA ID
MeSH Data
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Name
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ConceptScope note: The initial culturing of cells derived directly from fresh TISSUES.
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Tree numbers
- E01.370.225.500.223.500
- E05.200.500.265.500
- E05.242.223.500
- E05.481.500.249.500
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Qualifiersethics, trends, veterinary, history, classification, economics, instrumentation, methods, standards, statistics & numerical data