Procathepsin K, mouse recombinant

• Catalog number
  1027-10
• Price
  Please ask
• Size
  10 ug

• Synonyms
  Cathepsin O, Cathepsin O2, Cathepsin X, CTSK, CTSO, CTSO2
• Alternative_names
  Cathepsin O, Cathepsin O2, Cathepsin X, CTSK, CTSO, CTSO2
• Description
  Predominant proteinase responsible for the resorption of the bone matrix.
• Recombinant
  Yes
• Source
  E. coli.
• Purity by SDS PAGE
  ≥95%
• Assay
  SDS-PAGE
• Activity Specifications test method
  >1000 mU/mg (1 U = 1 µmole/min/mg) as measured by Cathepsin K Activity Assay Kit (Catalog #K141-100).
• Unit Definition
  1 U = 1 µmole/min/mg
• Molecular Weight
  35.5 kDa
• Storage Temp
  -80°C
• Shipping
  Dry ice
• Shelf Life
  12 months
• Concentration
  2.5 mg/ml
• Appearance
  Liquid
• Physical form description
  2.5 mg/ml solution in 25 mM Na2HPO4 and 500 mM NaCl (pH 7.0).
• Background Information
  Cathepsin K is a member of the papain cysteine proteinase family and has been identified as the predominant proteinase responsible for the resorption of the bone matrix. The enzyme cleaves proteins such as collagen type I, collagen type II and osteonectin and therefore plays a role in bone remodeling and resorption in diseases such as osteoporosis, osteolytic bone metastasis and rheumatoid arthritis (Bromme and Okamoto, 1995; Drake, F. et al 1996; Bossard et al, 1996). Cathepsin K is synthesized as an inactive proenzyme (35.1 kDa) that is converted to its mature active form (23.6 kDa) by proteolytic cleavage of its 99-amino-acid propeptide domain. The in-vitro processing of procathepsin K to mature cathepsin K is autocatalytic.
• Handling
  Centrifuge the vial prior to opening.
• Usage
  For Research Use Only! Not to be used in humans
• Datasheet
• Test
  Mouse or mice from the Mus musculus species are used for production of mouse monoclonal antibodies or mabs and as research model for humans in your lab. Mouse are mature after 40 days for females and 55 days for males. The female mice are pregnant only 20 days and can give birth to 10 litters of 6-8 mice a year. Transgenic, knock-out, congenic and inbread strains are known for C57BL/6, A/J, BALB/c, SCID while the CD-1 is outbred as strain.
• Latin name
  Mus musculus
• Additional source
  Recombinants or rec. proteins
• Group
  recombinants

• Gene target
  Procathepsin  
• Short name
  Procathepsin K, mouse recombinant
• Technique
  Recombinant, Mouse, mouses, E. coli recombinant proteins are genetic recombinations in Escherichia coli, supplied as white sterile powder lyopillized. Biovision advises they will be reconstituted in a buffer soluion or culture medium for cell culture.
• Host
  mouse
• Species
  Mouse, Mouses
• Alternative name
  Procathepsin K, mouse Rec.
• Alternative technique
  rec, murine

MeSH Data

• Name
  Primary Cell Culture
• Concept
  Scope note: The initial culturing of cells derived directly from fresh TISSUES.
• Tree numbers
  • E01.370.225.500.223.500
  • E05.200.500.265.500
  • E05.242.223.500
  • E05.481.500.249.500
• Qualifiers
  ethics, trends, veterinary, history, classification, economics, instrumentation, methods, standards, statistics & numerical data

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