PRF1 Polyclonal Antibody, Biotin Conjugated

Note

For research use only.
Properties

If you buy Antibodies supplied by EpiGentek they should be stored frozen at - 24°C for long term storage and for short term at + 5°C. Biotin conjugates can be detected by horseradish peroxidase, alkaline phosphatase substrates or anti biotin conjugated antibodies. Avidin and Streptavidin bind to the small biotin and are couple to HRP or AP for ELISA. To break the streptavidin Biotin bond we suggest to use a 6 molar guanidine HCl solution with acidity of pH 1.6.
Conjugation

Biotinylated
Group

Polyclonals and antibodies
About

Polyclonals can be used for Western blot, immunohistochemistry on frozen slices or parrafin fixed tissues. The advantage is that there are more epitopes available in a polyclonal antiserum to detect the proteins than in monoclonal sera.
French translation

anticorps

Gene target

PRF1 Conjugated
Gene symbol

PRF1
Short name

PRF1 Polyclonal Antibody, Conjugated
Technique

Polyclonal, Antibody, antibodies against human proteins, antibodies for, antibody Conjugates, Polyclonal antibodies (pAbs) are mostly rabbit or goat antibodies that are secreted by different B cells, whereas monoclonal antibodies come from a single N cell lineage. Pabs are a collection of immunoglobulin molecules that react against a specific antigen, each identifying a different epitope.
Label

Biotin
Alternative name

perforin 1 (pore forming protein) polyclonal (antibody to-), biotinilated coupled
Alternative technique

polyclonals, antibodies
Alternative to gene target

perforin 1 (pore forming protein), FLH2 and HPLH2 and P1 and PFN1 and PFP, PRF1 and IDBG-77451 and ENSG00000180644 and 5551, wide pore channel activity, Extracellular, Prf1 and IDBG-155739 and ENSMUSG00000037202 and 18646, BT.21975 and IDBG-629772 and ENSBTAG00000001008 and 369025

Identity

HGNC:9360
Gene

PRF1
Long gene name

perforin 1
Synonyms gene name
- perforin 1 (pore forming protein)
Synonyms
- PFP
- P1
- HPLH2
Synonyms name
- Perforin
- perforin 1 (preforming protein)
GenBank acession
- BC047695
Locus

10q22.1
Discovery year

1989-02-23
Entrez gene record

5551
Pubmed identfication
- 1505959
- 2592021
RefSeq identity
- NM_005041
Classification
- C2 domain containing
VEGA ID

OTTHUMG00000018412
Locus Specific Databases
- PRF1base: Mutation registry for Familiar haemophagocytic lymphohistiocytosis, type II (FHL2)
- LRG_94

Name

Blotting, Western
Concept

Scope note: Identification of proteins or peptides that have been electrophoretically separated by blot transferring from the electrophoresis gel to strips of nitrocellulose paper, followed by labeling with antibody probes.
Tree numbers
- E05.196.401.143
- E05.301.300.096
- E05.478.566.320.200
- E05.601.262
- E05.601.470.320.200
Qualifiers

ethics, trends, veterinary, history, classification, economics, instrumentation, methods, standards, statistics & numerical data