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Available ordering format
Lyophilized
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Immunogen
GST fusion to Pre-apoplastocyanin of Chlamydomonas reinhartii P18068
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Raised in
Rabbit
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Clonality
Polyclonal
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Clone
Polyclonal
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Purification
Serum
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How to reconstitute
For reconstitution add 200 µl of sterile water.
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Storage condition
store lyophilized/reconstituted at -20°C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please, remember to spin tubes briefly prior to opening them to avoid any losses that might occur from lyophilized material adhering to the cap or sides of the tubes.
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Verified applications
western blot (WB)
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Connected products
AS06 141 | anti-plastocyaninCollection of antibodies to Chlamydomonas proteinsAlgal protein extraction buffer Secondary antibodies
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Recommended dilutions for use
1: 1000 with alkaline phosphatase (WB)
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Molecular weight expected аpparent
17 kDa (precursor)
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Verified reactivity
Chlamydomonas reinhardtii
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Possible reactivity
Chlorella fusca Pediastrum boryanum
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No reactivity
no confirmed exceptions from predicted reactivity known in the moment
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Supplementary information
This antibody is recognizing pre-apoplatosyanin 17 kDa precursor and a mature protein. There is a slight cross-reactivity with cytochreom c6 in extract from Chlamydomonas reinhardtii cells grown under conditions of cooper deficiency.
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References
Li et al. (1996). Molecular genetic analysis of plastocyanin biosynthesis in Chlamydomonas reinhardtii.. J. Biol. Chem. 271:31283-31289
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Scientific context
Plastocyanin is a -œblue- copper protein which catalyzes electron transfer between the cytochrome b6 .f complex and P-700, the reaction center of photosystem I. Plastocyanin is a nuclear encoded polypeptide in all eukaryotic photosynthetic organisms where it has been studied. It is synthesized as a pre-protein of approximate molecular weight 17,000, imported post-translationally into chloroplasts, and processed to its mature form of approximate molecular weight 10,500 within the plastid.
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Notes
None
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Protein number
P18068
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TAIR number
Refer to NCBI
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