PPI, papain, Biotin conjugated

  • Catalog number
    AS09 546
  • Price
    Please ask
  • Size
    1 ml
  • Available ordering format
    Lyophilized in phosphate buffer saline
  • Immunogen
    native papain isolated and purified from Carica papaya
  • Raised in
    Rabbit
  • Clonality
    Polyclonal
  • Clone
    Polyclonal
  • Purification
    Purified IgG
  • How to reconstitute
    For reconstitution please add 1 ml of sterile destilled water.
  • Storage condition
    store lyophilized/reconstituted at -20°C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please, remember to spin tubes briefly prior to opening them to avoid any losses that might occur from lyophilized material adhering to the cap or sides of the tubes.
  • Verified applications
    western blot (WB), ELISA (ELISA),immunofluorescence (IF), immunohistochemistry (IHC)
  • Connected products
    AS09 545 | anti-PPI | papainAS09 547 | anti-PPI | papain (affinity purified)Plant protein extraction buffer
  • Recommended dilutions for use
    1: 1000 - 1: 100 000 for techniques listed above
  • Molecular weight expected аpparent
    38.9 kDa
  • Verified reactivity
    Carica papaya
  • Possible reactivity
    Carica papaya
  • No reactivity
    no confirmed exceptions from predicted reactivity known in the moment
  • Supplementary information
    Biotin/IgG protein molar ration is approximately 6.2. No foreign proteins are added.
  • References
    to be added when available
  • Scientific context
    Papain (EC=3.4.22.2) is a cysteine protease which belongs to peptidase C1 family. Can cause an allergic reaction in humans. Alternative names: Papaya proteinase I, allergen= Car p 1.
  • Notes
    Antibody is labelled with biotin using N-hydroxysuccinimidobiotin. Antibody potency and purity has been evaluated by immunoelectrophoresis, single radial immunodiffusion (Ouchterlony), ELISA,immunoblotting and enzyme inhibition.
  • Protein number
    Refer to NCBI
  • TAIR number
    Refer to NCBI
  • Properties
    Biotin conjugates can be detected by horseradish peroxidase, alkaline phosphatase substrates or anti biotin conjugated antibodies. Avidin and Streptavidin bind to the small biotin and are couple to HRP or AP for ELISA. To break the streptavidin Biotin bond we suggest to use a 6 molar guanidine HCl solution with acidity of pH 1.6.
  • Conjugation
    Biotinylated
  • Gene target
    PPI   papain   conjugated  
  • Short name
    PPI, papain, conjugated
  • Technique
    antibody Conjugates
  • Label
    Biotin
  • Alternative name
    PPI, papain, biotinilated coupled
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