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ELISA category
Streptavidin solution with azide
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ELISA detection limits
N/A
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ELISA sensitivity
N/A
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ELISA s description
BioTeZ offers a polymerized Streptavidin (molecular weight >2.000 kDa), the Polystreptavidin R, characterized by an extraordinary high Biotin binding capacity. Polystreptavidin R is a coating solution concentrate. It has a very high chemo- and thermostability, excellent adsorption capabilities and low unspecific bindings. Polystreptavidin R coatings serve the functionalization of surfaces to bind biotinylated compounds with a Biotin binding capacity of the type "Maximum Capacity”. It is very suitable for adsorptive coating membranes, beads, microfluidic systems, biochips, microplates etc. The coating surfaces may be made of the following materials: Nitrocellulose, ceramics or plastics like Polystyrene, Polyethylene, Polypropylene, Polycarbonate, Poly(methyl methacrylate) PMMA or Polyamides. Of particular note are the enormous savings of material and costs when using Polystreptavidin R instead of Streptavidin for coating membranes for lateral flow tests.
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Research area interests
all immuno assays
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Package form
solution
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Antigen
Biotin
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Reacts with
Biotin
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Tested applications
Use Polystreptavidin for coating of solid phases (e.g., membranes, microplates, beads etc.) as well with biotin-labeled haptens and antibodies in universal immunological detection systems. Polystreptavidin R is to be used in diluted form. For this, use a neutral buffer solution (e.g. phosphate buffered saline, PBS). Avoid basic solutions above pH 8. The coating concentration has to be optimized for the respective application. For life science research only. Not for use in diagnostic procedures.
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Specificity
Biotin
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Crossreactivity
N/A
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Preservative materials
If applicable mentioned in the product data sheet
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Storage recommendations
24 month
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Shipping and storage conditions
ice pack to temperature >25°C
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Long term storage
2-8°C
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Assay principle
Polystreptavidin is a coating compound to immobilize biotinylated molecules
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Included in the standard package
N/A
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Not included in the standard package
N/A
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Samples to be analyzed
coating material: microplates, beads, membranes etc. made Nitrocellulose, ceramics or plastics like Polystyrene, Polyethylene, Polypropylene, Polycarbonate, Poly(methyl methacrylate) PMMA or Polyamides
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PubMed citations
See the datasheet
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Technical datasheet
Contact us to receive the datasheet
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Representative figures
No Available
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Warnings
For Research Use only.
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Description
Sodium azide NaN3 is often used to prevent antibody degradation in 0,02% W/V concentrations. biotez supplies azide free antibodies on request. The volume (ml) in milliliters of buffered % w/v solutions at the medium pH are also used for DNA extraction Organic (Phenol-Chloroform) Extraction, Non-Organic (Proteinase K and Salting out), Chelex (Ion Exchange Resin) Extraction, EDTA or PBS aqueous.
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Properties
Biotin conjugates can be detected by horseradish peroxidase, alkaline phosphatase substrates or anti biotin conjugated antibodies. Avidin and Streptavidin bind to the small biotin and are couple to HRP or AP for ELISA. To break the streptavidin Biotin bond we suggest to use a 6 molar guanidine HCl solution with acidity of pH 1.6.
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Conjugation
Biotinylated
